Transcript: Human XM_017014952.2

PREDICTED: Homo sapiens DENN domain containing 1A (DENND1A), transcript variant X14, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DENND1A (57706)
Length:
5150
CDS:
941..3376

Additional Resources:

NCBI RefSeq record:
XM_017014952.2
NBCI Gene record:
DENND1A (57706)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017014952.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000249257 ATTCGGGTTCTGCCGCTTATC pLKO_005 458 5UTR 100% 10.800 15.120 N Dennd1a n/a
2 TRCN0000350941 ATTCGGGTTCTGCCGCTTATC pLKO_005 458 5UTR 100% 10.800 15.120 N DENND1A n/a
3 TRCN0000142109 GAACGCCGGATACTCATCATT pLKO.1 789 5UTR 100% 5.625 7.875 N DENND1A n/a
4 TRCN0000141851 GATCTTCTCAATTCCGGCGAA pLKO.1 1280 CDS 100% 2.160 3.024 N DENND1A n/a
5 TRCN0000144938 GAAGTGGAGCAATTCTGAATA pLKO.1 1395 CDS 100% 13.200 9.240 N DENND1A n/a
6 TRCN0000143383 GCTTAACATCCTGGCAGATTA pLKO.1 545 5UTR 100% 13.200 9.240 N DENND1A n/a
7 TRCN0000201104 CCAGCATACCTGAGAATAGAA pLKO.1 700 5UTR 100% 5.625 3.938 N Dennd1a n/a
8 TRCN0000141720 GTTGCATCTGTACGCCAGTAT pLKO.1 761 5UTR 100% 4.950 3.465 N DENND1A n/a
9 TRCN0000338625 GTTGCATCTGTACGCCAGTAT pLKO_005 761 5UTR 100% 4.950 3.465 N DENND1A n/a
10 TRCN0000122398 GACTGTCTACAAGTTCGCAAA pLKO.1 1447 CDS 100% 4.050 2.835 N DENND1A n/a
11 TRCN0000141749 GCAAACAGAGATTCGGGTTCT pLKO.1 448 5UTR 100% 4.050 2.835 N DENND1A n/a
12 TRCN0000141748 GATCCTGAATGTGGACACCAA pLKO.1 961 CDS 100% 2.640 1.848 N DENND1A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017014952.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03844 pDONR223 100% 26.7% 25.3% None (many diffs) n/a
2 ccsbBroad304_03844 pLX_304 0% 26.7% 25.3% V5 (many diffs) n/a
3 TRCN0000481037 TATGGCCGATTTACATGACTCTCC pLX_317 25.9% 26.7% 25.3% V5 (many diffs) n/a
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