Transcript: Human XM_017018700.1

PREDICTED: Homo sapiens calcium/calmodulin dependent protein kinase kinase 2 (CAMKK2), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CAMKK2 (10645)
Length:
4916
CDS:
132..1811

Additional Resources:

NCBI RefSeq record:
XM_017018700.1
NBCI Gene record:
CAMKK2 (10645)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017018700.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196992 GCAAAGAGGACGCCCATAATT pLKO.1 4223 3UTR 100% 15.000 21.000 N CAMKK2 n/a
2 TRCN0000195473 CCCGATGCTTCTGTTTCATTC pLKO.1 4724 3UTR 100% 10.800 15.120 N CAMKK2 n/a
3 TRCN0000363096 TCGTCAAGTTGGCCTACAATG pLKO_005 718 CDS 100% 10.800 15.120 N CAMKK2 n/a
4 TRCN0000002301 CGACCCTTTCTACTATGCATT pLKO.1 4746 3UTR 100% 4.950 6.930 N CAMKK2 n/a
5 TRCN0000002300 AGTCAAACACATTCCCAGCTT pLKO.1 1607 CDS 100% 2.640 3.696 N CAMKK2 n/a
6 TRCN0000363122 ATCGTCATCTCTGGTTATTTG pLKO_005 2022 3UTR 100% 13.200 9.240 N CAMKK2 n/a
7 TRCN0000363121 GCCATGGGTGTGACACTATAC pLKO_005 1308 CDS 100% 10.800 7.560 N CAMKK2 n/a
8 TRCN0000002299 GTGAAGACCATGATACGTAAA pLKO.1 1644 CDS 100% 10.800 7.560 N CAMKK2 n/a
9 TRCN0000194684 CAATACCTACTATGCAATGAA pLKO.1 746 CDS 100% 5.625 3.938 N CAMKK2 n/a
10 TRCN0000002297 CGAGCGGATCATGTGTTTACA pLKO.1 1361 CDS 100% 5.625 3.938 N CAMKK2 n/a
11 TRCN0000002298 CCGTTTCTACTTCCAGGATCT pLKO.1 1052 CDS 100% 4.050 2.835 N CAMKK2 n/a
12 TRCN0000199090 CCTCTCATCCTTGAGCATCCA pLKO.1 248 CDS 100% 2.640 1.848 N CAMKK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017018700.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489004 CTGAGTCACCCCACTTCCGCACTA pLX_317 17.4% 96.6% 96.7% V5 (not translated due to prior stop codon) 520_573del;684C>T;1017A>T n/a
2 TRCN0000489407 TCATTGGCTCTTTCTTGGTCAGCC pLX_317 18.3% 96.6% 96.6% V5 (many diffs) n/a
3 TRCN0000491777 CTGATCGGTCACTTAGGGTCTCAT pLX_317 27.7% 88.9% 88.9% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000474789 TAATACCAGACATGCCCCAAAATT pLX_317 64.5% 33.9% 2.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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