Construct: ORF TRCN0000474789
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018858.3_s317c1
- Derived from:
- ccsbBroadEn_14970
- DNA Barcode:
- TAATACCAGACATGCCCCAAAATT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CAMKK2 (10645)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474789
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10519 | CIB1 | calcium and integrin binding 1 | NM_006384.4 | 61.6% | 40.4% | (many diffs) |
| 2 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XM_017018709.2 | 59.7% | 7.3% | (many diffs) |
| 3 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XM_024448799.1 | 59.7% | 7.3% | (many diffs) |
| 4 | human | 10519 | CIB1 | calcium and integrin binding 1 | XM_006720375.2 | 54.2% | 29.3% | (many diffs) |
| 5 | human | 10519 | CIB1 | calcium and integrin binding 1 | NM_001277764.1 | 52.7% | 33.4% | (many diffs) |
| 6 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XM_024448798.1 | 50% | 2.9% | (many diffs) |
| 7 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XM_017018706.1 | 45.3% | 5.8% | (many diffs) |
| 8 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XM_017018708.1 | 44.8% | 4.8% | (many diffs) |
| 9 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XM_011537764.2 | 44.6% | 5.4% | (many diffs) |
| 10 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | NM_153499.2 | 35% | 2.1% | (many diffs) |
| 11 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | NM_172226.2 | 35% | 2.1% | (many diffs) |
| 12 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XM_017018700.1 | 33.9% | 2.1% | (many diffs) |
| 13 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | NM_001270485.2 | 32.3% | 3.9% | (many diffs) |
| 14 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | NM_006549.3 | 32.3% | 3.9% | (many diffs) |
| 15 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | NM_001270486.1 | 32.2% | 4.1% | (many diffs) |
| 16 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XM_011537763.1 | 32.2% | 4.1% | (many diffs) |
| 17 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XM_017018702.2 | 32.2% | 4.1% | (many diffs) |
| 18 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | NM_172214.3 | 31.6% | 3.4% | (many diffs) |
| 19 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XM_005253823.1 | 31.6% | 3.4% | (many diffs) |
| 20 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XM_017018694.1 | 31.3% | 3.7% | (many diffs) |
| 21 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XM_017018695.1 | 31.3% | 3.7% | (many diffs) |
| 22 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XM_017018703.1 | 30.6% | 3.3% | (many diffs) |
| 23 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XR_001748557.1 | 11.8% | (many diffs) | |
| 24 | human | 10645 | CAMKK2 | calcium/calmodulin dependen... | XR_001748558.1 | 11.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 321
- ORF length:
- 252
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggggctcg ggcagtcgcc tgtccaagga gctgctggcc gagtaccagg 121 acttgacgtt cctgacgaag caggagatcc tcctagccca caggcggttt tgtgagctgc 181 ttccccagga gcagcggagc gtggagtcgt cacttcgggc acaagtgccc ttcgagcaga 241 ttctcagcct tccagagctc aaggccaacc ccttcaagga gcgaatctTC AGGCCCTGGA 301 ATTTCCAGAC CAGCCCGACA TAGCTGAGGA CTTGAAGGAC CTGATCACCC GTATGCTGGA 361 CAAGAACCCC GAGTCGAGGA TCGTGGTGCC GGAAATCAAG CTGCACCCCT GGGTCACGAG 421 GCATGGGGCG GAGCCGTTGC CGTCGGAGGA TGAGAACTGC ACGCTGGTCG AAGTGACTGA 481 AGAGGAGGTC GAGAACTCAG TCAAACACAT TCCCAGCTTG GCAACCGTGA TCCTGGTGAA 541 GACCATGATA CGTAAACGCT CCTTTGGGAA CCCATTCGAG GGCAGCCGGC GGGAGGAACG 601 CTCACTGTCA GCGCCTGGAA ACTTGCTCAC GAAGCAAGGC AGCGAAGACA ACCTCCAGGG 661 CACCGACCCG CCCCCCGTGG GGGAGGAGGA AGTGCTCTTG TTGCCAACTT TCTTGTACAA 721 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 781 ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 841 GGACGATAAT ACCAGACATG CCCCAAAATT ACGCGTTAAG TCgacaatca acctctggat 901 tacaaaattt gtgaaagatt