Transcript: Human XM_017020244.1

PREDICTED: Homo sapiens WSC domain containing 2 (WSCD2), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
WSCD2 (9671)
Length:
4927
CDS:
1454..3151

Additional Resources:

NCBI RefSeq record:
XM_017020244.1
NBCI Gene record:
WSCD2 (9671)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017020244.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000418606 GAAGTGCTGGCACCGATGTTT pLKO_005 3623 3UTR 100% 5.625 4.500 N WSCD2 n/a
2 TRCN0000158682 GCATCAATCTTACAAAGTGTT pLKO.1 4302 3UTR 100% 4.950 3.960 N WSCD2 n/a
3 TRCN0000434381 GGACTCCTAGTTACTTCATTG pLKO_005 2391 CDS 100% 10.800 7.560 N WSCD2 n/a
4 TRCN0000414252 CTGCATCAAGACGCACGAAAG pLKO_005 2641 CDS 100% 6.000 4.200 N WSCD2 n/a
5 TRCN0000158615 CCACCTTCTGTTCTAAAGAAA pLKO.1 3652 3UTR 100% 5.625 3.938 N WSCD2 n/a
6 TRCN0000164402 CAGGTATGGAAGTGCAGTGTT pLKO.1 2131 CDS 100% 4.950 3.465 N WSCD2 n/a
7 TRCN0000163306 GCCTACATCAAGATGGTGGAT pLKO.1 3074 CDS 100% 2.640 1.848 N WSCD2 n/a
8 TRCN0000413809 GTCCCTTCTGGGCTGAGATTT pLKO_005 3575 3UTR 100% 13.200 7.920 N WSCD2 n/a
9 TRCN0000158652 CCTTCTGTTCTAAAGAAAGAT pLKO.1 3655 3UTR 100% 5.625 3.375 N WSCD2 n/a
10 TRCN0000164132 CCAGAAGGATGGCAACTTCAA pLKO.1 2989 CDS 100% 4.950 2.970 N WSCD2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017020244.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11399 pDONR223 100% 40.1% 40.1% None 1_990del;1343_1344ins60 n/a
2 ccsbBroad304_11399 pLX_304 0% 40.1% 40.1% V5 1_990del;1343_1344ins60 n/a
3 TRCN0000492263 GAAATGCAGGTCCGAAGGCAGATC pLX_317 45.3% 40.1% 40.1% V5 1_990del;1343_1344ins60 n/a
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