Transcript: Human XM_017023019.1

PREDICTED: Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ACSF3 (197322)
Length:
2923
CDS:
344..2011

Additional Resources:

NCBI RefSeq record:
XM_017023019.1
NBCI Gene record:
ACSF3 (197322)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017023019.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000231120 ACCCTCCGTGTTTCGAGAATA pLKO_005 1630 CDS 100% 13.200 10.560 N ACSF3 n/a
2 TRCN0000218624 TTATGGCAGTGCCTACAATAT pLKO_005 1212 CDS 100% 13.200 10.560 N ACSF3 n/a
3 TRCN0000157714 CATCAAGACTGGAGGCTACAA pLKO.1 1771 CDS 100% 4.950 3.465 N ACSF3 n/a
4 TRCN0000156573 GCTGGAGAAGTGGAAGAACAT pLKO.1 1360 CDS 100% 4.950 3.465 N ACSF3 n/a
5 TRCN0000156364 CAGGATTTCTTGCGTGCAGTT pLKO.1 1283 CDS 100% 4.050 2.835 N ACSF3 n/a
6 TRCN0000157272 GATGTGGCTGTGATTGGAGTT pLKO.1 1844 CDS 100% 4.050 2.835 N ACSF3 n/a
7 TRCN0000156574 GTGGACATCATCAAGACTGGA pLKO.1 1763 CDS 100% 2.640 1.848 N ACSF3 n/a
8 TRCN0000154382 CGGATCAATGTCTTTATGGCA pLKO.1 1199 CDS 100% 0.750 0.525 N ACSF3 n/a
9 TRCN0000231119 ACACGTACAGGGAGCTTTATT pLKO_005 540 CDS 100% 15.000 9.000 N ACSF3 n/a
10 TRCN0000156424 CATCATCAAGACTGGAGGCTA pLKO.1 1768 CDS 100% 2.640 1.584 N ACSF3 n/a
11 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 2510 3UTR 100% 4.950 2.475 Y CFLAR n/a
12 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 2510 3UTR 100% 4.950 2.475 Y C19orf31 n/a
13 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 2508 3UTR 100% 4.950 2.475 Y ERN2 n/a
14 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 2508 3UTR 100% 4.950 2.475 Y P3H4 n/a
15 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 2508 3UTR 100% 4.950 2.475 Y P3H4 n/a
16 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 2677 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017023019.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09794 pDONR223 100% 94.5% 91.4% None (many diffs) n/a
2 ccsbBroad304_09794 pLX_304 0% 94.5% 91.4% V5 (many diffs) n/a
3 TRCN0000477630 CGCGGCACAGGGACTCTTGGTCTT pLX_317 22.2% 94.5% 91.4% V5 (many diffs) n/a
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