Construct: ORF TRCN0000477630
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010270.1_s317c1
- Derived from:
- ccsbBroadEn_09794
- DNA Barcode:
- CGCGGCACAGGGACTCTTGGTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACSF3 (197322)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477630
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | NM_001127214.4 | 99.4% | 99.4% | (many diffs) |
| 2 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | NM_001243279.3 | 99.4% | 99.4% | (many diffs) |
| 3 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | NM_174917.5 | 99.4% | 99.4% | (many diffs) |
| 4 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | XM_017023019.1 | 94.5% | 91.4% | (many diffs) |
| 5 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | XM_005256293.2 | 90.7% | 83.9% | (many diffs) |
| 6 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | XM_011522942.1 | 90.7% | 83.9% | (many diffs) |
| 7 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | XM_011522943.1 | 90.7% | 83.9% | (many diffs) |
| 8 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | XM_017023018.1 | 90.7% | 83.9% | (many diffs) |
| 9 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | XM_017023021.1 | 79.6% | 78.1% | (many diffs) |
| 10 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | XR_933240.3 | 67.3% | (many diffs) | |
| 11 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | XR_001751864.2 | 62.4% | (many diffs) | |
| 12 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | NM_001284316.2 | 53.9% | 53.8% | 0_1ins795;319G>A |
| 13 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | XM_024450187.1 | 53.9% | 53.8% | 0_1ins795;319G>A |
| 14 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | XR_001751865.1 | 52.1% | (many diffs) | |
| 15 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | XM_024450186.1 | 48.2% | 42.9% | (many diffs) |
| 16 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | NR_104293.2 | 45.8% | (many diffs) | |
| 17 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | NR_147928.2 | 45.2% | (many diffs) | |
| 18 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | NR_147929.2 | 42.4% | (many diffs) | |
| 19 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | XM_017023022.1 | 38.4% | 27% | (many diffs) |
| 20 | human | 197322 | ACSF3 | acyl-CoA synthetase family ... | XR_933238.2 | 27% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1797
- ORF length:
- 1728
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccgccccat gtggtgctca ccttccggcg cctgggctgc gccttggcct 121 cctgccggct ggcgcctgcg agacacagag gaagtggtct tctgcacaca gccccagtgg 181 cccgctcgga caggagcgcc ccggtgttca cccgtgccct ggcctttggg gacagaatcg 241 ccctggttga ccagcacggc cgccacacgt acagggagct ttattcccgc agccttcgcc 301 tgtcccagga gatctgcagg ctctgcgggt gtgtcggcgg ggacctccgg gaggagaggg 361 tctccttcct atgtgctaac gacgcctcct acgtcgtggc ccagtgggcc tcatggatga 421 gcggcggtgt ggcagtaccc ctctacagga agcatcccgc ggcccagctg gagtatgtca 481 tctgcgactc ccagagctct gtggtccttg tcagccagga gtacctggag ctcctgagcc 541 cggtggtcag gaagctgggg gtcccgctgc tgccgctcac accagccatc tacactggag 601 cagtagagga accggcagag gtcccggtcc cagagcaggg atggaggaac aagggcgcca 661 tgatcatcta caccagtggg accacgggga ggcccaaggg cgtgctgagc acgcaccaaa 721 acatcagggc tgtggtgacc gggctggtcc acaagtgggc atggaccaaa gacgacgtga 781 tcctccacgt gctcccgctg caccacgtcc atggtgtggt caacgcgctg ctctgtcctc 841 tctgggtggg agccacctgt gtgatgatgc ctgagttcag ccctcagcag gtttgggaaa 901 agttcttaag ttctgaaacg ccgcggatca atgtctttat ggcagtgcct acaatataca 961 ccaagctgat ggagtactac gacaggcatt ttacccagcc gcacgcccag gatttcttgc 1021 gtgcagtttg tgaagaaaaa attaggctga tggtctcagg ctcagctgcc ctgcccctcc 1081 cagtgctgga gaagtggaag aacatcacgg gccacaccct gctggagcgg tatggcatga 1141 ccgagatcgg catggctctg tccgggcccc tgaccactgc catgcgcctg ccaggttccg 1201 tggggacccc actgcctgga gtacaggtgc gcattgtctc agaaaaccca cagagggaag 1261 cctgctccta caccatccac gcagagggag acgagagggg gaccaaggtg accccagggt 1321 ttgaagaaaa ggagggggag ctgctggtga ggggaccctc cgtgtttcga gaatactgga 1381 ataaaccaga agaaactaag agtgcattca ccctggatGG CTGGTTTAAG ACAGGGGACA 1441 CCGTGGTGTT TAAGGATGGC CAGTACTGGA TCCGAGGCCG GACCTCAGTG GACATCATCA 1501 AGACTGGAGG CTACAAGGTC AGCGCCCTGG AGGTGGAGTG GCACCTGCTG GCCCACCCCA 1561 GCATCACAGA TGTGGCTGTG ATTGGAGTTC CGGATATGAC ATGGGGCCAG CGGGTCACTG 1621 CTGTGGTGAC CCTCCGAGAA GGACACTCAC TGTCCCACAG GGAGCTCAAA GAGTGGGCCA 1681 GAAATGTCCT GGCCCCGTAC GCGGTGCCCT CGGAGCTGGT GCTGGTGGAG GAGATCCCGC 1741 GGAACCAGAT GGGCAAGATT GACAAGAAGG CGCTCATCAG GCACTTCCAC CCCTCATTGC 1801 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1861 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1921 ATATATCTTG TGGAAAGGAC GACGCGGCAC AGGGACTCTT GGTCTTACGC GTTAAGTCga 1981 caatcaacct ctggattaca aaatttgtga aagatt