Construct: ORF TRCN0000477630

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010270.1_s317c1
Derived from:
ccsbBroadEn_09794
DNA Barcode:
CGCGGCACAGGGACTCTTGGTCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACSF3 (197322)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477630
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 197322 ACSF3 acyl-CoA synthetase family ... NM_001127214.4 99.4% 99.4% (many diffs)
2 human 197322 ACSF3 acyl-CoA synthetase family ... NM_001243279.3 99.4% 99.4% (many diffs)
3 human 197322 ACSF3 acyl-CoA synthetase family ... NM_174917.5 99.4% 99.4% (many diffs)
4 human 197322 ACSF3 acyl-CoA synthetase family ... XM_017023019.1 94.5% 91.4% (many diffs)
5 human 197322 ACSF3 acyl-CoA synthetase family ... XM_005256293.2 90.7% 83.9% (many diffs)
6 human 197322 ACSF3 acyl-CoA synthetase family ... XM_011522942.1 90.7% 83.9% (many diffs)
7 human 197322 ACSF3 acyl-CoA synthetase family ... XM_011522943.1 90.7% 83.9% (many diffs)
8 human 197322 ACSF3 acyl-CoA synthetase family ... XM_017023018.1 90.7% 83.9% (many diffs)
9 human 197322 ACSF3 acyl-CoA synthetase family ... XM_017023021.1 79.6% 78.1% (many diffs)
10 human 197322 ACSF3 acyl-CoA synthetase family ... XR_933240.3 67.3% (many diffs)
11 human 197322 ACSF3 acyl-CoA synthetase family ... XR_001751864.2 62.4% (many diffs)
12 human 197322 ACSF3 acyl-CoA synthetase family ... NM_001284316.2 53.9% 53.8% 0_1ins795;319G>A
13 human 197322 ACSF3 acyl-CoA synthetase family ... XM_024450187.1 53.9% 53.8% 0_1ins795;319G>A
14 human 197322 ACSF3 acyl-CoA synthetase family ... XR_001751865.1 52.1% (many diffs)
15 human 197322 ACSF3 acyl-CoA synthetase family ... XM_024450186.1 48.2% 42.9% (many diffs)
16 human 197322 ACSF3 acyl-CoA synthetase family ... NR_104293.2 45.8% (many diffs)
17 human 197322 ACSF3 acyl-CoA synthetase family ... NR_147928.2 45.2% (many diffs)
18 human 197322 ACSF3 acyl-CoA synthetase family ... NR_147929.2 42.4% (many diffs)
19 human 197322 ACSF3 acyl-CoA synthetase family ... XM_017023022.1 38.4% 27% (many diffs)
20 human 197322 ACSF3 acyl-CoA synthetase family ... XR_933238.2 27% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1797
ORF length:
1728
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gccgccccat gtggtgctca ccttccggcg cctgggctgc gccttggcct
121cctgccggct ggcgcctgcg agacacagag gaagtggtct tctgcacaca gccccagtgg
181cccgctcgga caggagcgcc ccggtgttca cccgtgccct ggcctttggg gacagaatcg
241ccctggttga ccagcacggc cgccacacgt acagggagct ttattcccgc agccttcgcc
301tgtcccagga gatctgcagg ctctgcgggt gtgtcggcgg ggacctccgg gaggagaggg
361tctccttcct atgtgctaac gacgcctcct acgtcgtggc ccagtgggcc tcatggatga
421gcggcggtgt ggcagtaccc ctctacagga agcatcccgc ggcccagctg gagtatgtca
481tctgcgactc ccagagctct gtggtccttg tcagccagga gtacctggag ctcctgagcc
541cggtggtcag gaagctgggg gtcccgctgc tgccgctcac accagccatc tacactggag
601cagtagagga accggcagag gtcccggtcc cagagcaggg atggaggaac aagggcgcca
661tgatcatcta caccagtggg accacgggga ggcccaaggg cgtgctgagc acgcaccaaa
721acatcagggc tgtggtgacc gggctggtcc acaagtgggc atggaccaaa gacgacgtga
781tcctccacgt gctcccgctg caccacgtcc atggtgtggt caacgcgctg ctctgtcctc
841tctgggtggg agccacctgt gtgatgatgc ctgagttcag ccctcagcag gtttgggaaa
901agttcttaag ttctgaaacg ccgcggatca atgtctttat ggcagtgcct acaatataca
961ccaagctgat ggagtactac gacaggcatt ttacccagcc gcacgcccag gatttcttgc
1021gtgcagtttg tgaagaaaaa attaggctga tggtctcagg ctcagctgcc ctgcccctcc
1081cagtgctgga gaagtggaag aacatcacgg gccacaccct gctggagcgg tatggcatga
1141ccgagatcgg catggctctg tccgggcccc tgaccactgc catgcgcctg ccaggttccg
1201tggggacccc actgcctgga gtacaggtgc gcattgtctc agaaaaccca cagagggaag
1261cctgctccta caccatccac gcagagggag acgagagggg gaccaaggtg accccagggt
1321ttgaagaaaa ggagggggag ctgctggtga ggggaccctc cgtgtttcga gaatactgga
1381ataaaccaga agaaactaag agtgcattca ccctggatGG CTGGTTTAAG ACAGGGGACA
1441CCGTGGTGTT TAAGGATGGC CAGTACTGGA TCCGAGGCCG GACCTCAGTG GACATCATCA
1501AGACTGGAGG CTACAAGGTC AGCGCCCTGG AGGTGGAGTG GCACCTGCTG GCCCACCCCA
1561GCATCACAGA TGTGGCTGTG ATTGGAGTTC CGGATATGAC ATGGGGCCAG CGGGTCACTG
1621CTGTGGTGAC CCTCCGAGAA GGACACTCAC TGTCCCACAG GGAGCTCAAA GAGTGGGCCA
1681GAAATGTCCT GGCCCCGTAC GCGGTGCCCT CGGAGCTGGT GCTGGTGGAG GAGATCCCGC
1741GGAACCAGAT GGGCAAGATT GACAAGAAGG CGCTCATCAG GCACTTCCAC CCCTCATTGC
1801CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1861TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1921ATATATCTTG TGGAAAGGAC GACGCGGCAC AGGGACTCTT GGTCTTACGC GTTAAGTCga
1981caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)