Transcript: Human XM_017027231.1

PREDICTED: Homo sapiens zinc finger protein 69 (ZNF69), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZNF69 (7620)
Length:
832
CDS:
152..652

Additional Resources:

NCBI RefSeq record:
XM_017027231.1
NBCI Gene record:
ZNF69 (7620)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017027231.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000148059 GCCTATGAGTATCAGGAATAT pLKO.1 617 CDS 100% 13.200 7.920 N ZNF69 n/a
2 TRCN0000147547 GAAACTCTACAAGGAAGTGAT pLKO.1 298 CDS 100% 4.950 2.970 N ZNF69 n/a
3 TRCN0000149207 GTATCAGGAATATGGACCGAA pLKO.1 625 CDS 100% 2.640 1.584 N ZNF69 n/a
4 TRCN0000149143 GAGAAACTTCAGGAGTCTCAT pLKO.1 400 CDS 100% 4.950 2.475 Y ZNF69 n/a
5 TRCN0000149677 GATGCTGGAAACTTTCAGGAA pLKO.1 316 CDS 100% 2.640 1.320 Y ZNF69 n/a
6 TRCN0000156989 GCTGGAAACTTTCAGGAACCT pLKO.1 319 CDS 100% 2.640 1.320 Y ZNF763 n/a
7 TRCN0000016371 GCTGGATATTTCCCAGAGGAA pLKO.1 280 CDS 100% 2.640 1.320 Y ZNF440 n/a
8 TRCN0000107953 GCTGTGAACTTCACCCAGGAA pLKO.1 248 CDS 100% 2.640 1.320 Y ZNF799 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017027231.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01804 pDONR223 100% 89.7% 89.7% None 63_113del n/a
2 ccsbBroad304_01804 pLX_304 0% 89.7% 89.7% V5 63_113del n/a
3 TRCN0000471005 ACATCAGGGGTCAATTGTCCTCTC pLX_317 27.5% 29.1% 28.1% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_15236 pDONR223 77.6% 22.2% 21.5% None (many diffs) n/a
5 ccsbBroad304_15236 pLX_304 0% 22.2% 21.5% V5 (many diffs) n/a
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