Construct: ORF TRCN0000471005
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009548.1_s317c1
- Derived from:
- ccsbBroadEn_15236
- DNA Barcode:
- ACATCAGGGGTCAATTGTCCTCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF440 (126070)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471005
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348720.1 | 86.1% | 79% | (many diffs) |
| 2 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348721.1 | 86.1% | 79% | (many diffs) |
| 3 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_152262.2 | 83.8% | 77% | (many diffs) |
| 4 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348719.1 | 83% | 76.4% | (many diffs) |
| 5 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348718.1 | 82.5% | 75.7% | (many diffs) |
| 6 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348722.1 | 80% | 73.4% | (many diffs) |
| 7 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348723.1 | 80% | 73.4% | (many diffs) |
| 8 | human | 7620 | ZNF69 | zinc finger protein 69 | NM_001364731.1 | 75.8% | 70.6% | (many diffs) |
| 9 | human | 284390 | ZNF763 | zinc finger protein 763 | NM_001367172.2 | 75.7% | 67.1% | (many diffs) |
| 10 | human | 284390 | ZNF763 | zinc finger protein 763 | NM_001367173.2 | 75.3% | 66.5% | (many diffs) |
| 11 | human | 126070 | ZNF440 | zinc finger protein 440 | NM_152357.3 | 75.3% | 75.4% | 1346_1785del |
| 12 | human | 284390 | ZNF763 | zinc finger protein 763 | NM_001012753.2 | 75.2% | 66.4% | (many diffs) |
| 13 | human | 7620 | ZNF69 | zinc finger protein 69 | NM_001364730.1 | 75% | 71.5% | (many diffs) |
| 14 | human | 126070 | ZNF440 | zinc finger protein 440 | XM_005259731.4 | 74.9% | 74.9% | 1_3delATG;6_11delGTTTCA;1355_1794del |
| 15 | human | 7620 | ZNF69 | zinc finger protein 69 | XM_006722873.4 | 74.6% | 71% | (many diffs) |
| 16 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348724.1 | 62.4% | 57.1% | (many diffs) |
| 17 | human | 90594 | ZNF439 | zinc finger protein 439 | NM_001348725.1 | 62.4% | 57.1% | (many diffs) |
| 18 | human | 90592 | ZNF700 | zinc finger protein 700 | NM_144566.3 | 55.1% | 50.1% | (many diffs) |
| 19 | human | 90592 | ZNF700 | zinc finger protein 700 | NM_001271848.1 | 54.9% | 49.7% | (many diffs) |
| 20 | human | 126070 | ZNF440 | zinc finger protein 440 | XM_017026254.1 | 54.8% | 54.9% | 0_1ins366;980_1419del |
| 21 | human | 7620 | ZNF69 | zinc finger protein 69 | NM_001321163.2 | 29.3% | 28.5% | (many diffs) |
| 22 | human | 7620 | ZNF69 | zinc finger protein 69 | NM_021915.4 | 29.2% | 26.5% | (many diffs) |
| 23 | human | 7620 | ZNF69 | zinc finger protein 69 | XM_006722874.3 | 29.1% | 28.1% | (many diffs) |
| 24 | human | 7620 | ZNF69 | zinc finger protein 69 | XM_017027231.1 | 29.1% | 28.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1413
- ORF length:
- 1347
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cccagtggct tttaaggatg tggctgtgaa cttcacccag gaggagtggg 121 ctttgctgga tatttcccag aggaaactct acagggaagt gatgctggaa actttcagga 181 acctgacctc tttaggaaaa aggtggaaag accagaacat tgaatatgag caccaaaacc 241 ccaggagaaa cttcaggagt ctcatagaag aaaaagtcaa tgaaattaaa gatgacagtc 301 attgtggaga aacttttacc ccagttccag atgacagact gaacttccag gagaagaaag 361 cttctcctga agtaaaatca tgtgaaagct ttgtgtgtgg agaagttggc ctaggtaact 421 catcttttaa tatgaacatc agaggtgaca ttggacacaa ggcatatgag tatcaggaat 481 atggaccaaa gccatgtaag tgtcaacaac ctaaaaaagc cttcagatac cgcccctcct 541 ttagaacaca agaaagggat cacactggag agaaacccaa tgcttgtaaa gtatgtggaa 601 aaacctttat ttcccattca agtgttcgaa gacacatggt aatgcacagt ggggatggac 661 cttataaatg taagttttgt gggaaagcat tccattgtct cagattatat cttatccatg 721 aaagaattca cactggagag aaaccatgtg aatgtaaaca gtgtggtaaa tcctttagtt 781 attctgctac ccatcgaata cataaaagaa ctcacactgg agaaaagcct tatgaatatc 841 aggagtgtgg gaaagcattt catagtccca gatcctatcg tagacatgaa aggattcaca 901 tgggagaaaa ggcttatcaa tgtaaggaat gtggaaaagc attcacgtgt ccccgttatg 961 ttcgtataca tgaaaggacc cactctagga aaaatctcta tgaatgtaag cagtgtggga 1021 aagcattatc ctctcttaca agtttTCAAA CACACGTAAG ATTGCACTCT GGAGAAAGAC 1081 CTTATGAATG TAAGATATGT GGAAAAGACT TTTGTTCTGT GAATTCATTT CAAAGACATG 1141 AAAAAATTCA CAGTGGAGAG AAACCCTATA AATGTAAGCA GTGTGGTAAA GCCTTCCCTC 1201 ATTCCAGTTC CCTTCGATAT CATGAAAGGA CTCACACTGG AGAGAAACCC TATGAGTGTA 1261 AGCAATGTGG GAAAGCCTTC AGATCTGCCT CACACCTTCG AGTGCATGGT AGGACTCACA 1321 CTGGAGAGAA ACCGTATGAA TGTAAGGAAT GTGGGAAAGC CTTCAGATAT GTGAATAACC 1381 TTCAAAGTCA TGAAAGGACA CAAACACACA TACCCAACTT TCTTGTACAA AGTGGTTGAT 1441 ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT 1501 CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACAT 1561 CAGGGGTCAA TTGTCCTCTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt 1621 gtgaaagatt