Transcript: Human XM_017029559.1

PREDICTED: Homo sapiens p21 (RAC1) activated kinase 3 (PAK3), transcript variant X11, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PAK3 (5063)
Length:
2634
CDS:
481..2124

Additional Resources:

NCBI RefSeq record:
XM_017029559.1
NBCI Gene record:
PAK3 (5063)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148181 CTTACTTACAGTGAATTCCC pXPR_003 CGG 268 16% 4 0.8634 PAK3 PAK3 75679
2 BRDN0001148041 GGTCTTGGTGCGATAACTGG pXPR_003 TGG 567 34% 7 0.1665 PAK3 PAK3 75678
3 BRDN0001148485 TTTGCAGATAAAAGTGCACA pXPR_003 TGG 440 27% 6 -0.9876 PAK3 PAK3 75680
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017029559.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199531 GTCAGCTGTATTCCGTGACTT pLKO.1 1944 CDS 100% 4.950 6.930 N PAK3 n/a
2 TRCN0000199628 GCACGATTACTCCAAACTTCC pLKO.1 775 CDS 100% 4.050 5.670 N PAK3 n/a
3 TRCN0000003243 CAACCCAAGAAGGAATTAATT pLKO.1 1390 CDS 100% 15.000 10.500 N PAK3 n/a
4 TRCN0000195548 CAGACTTTGAGCATACGATTC pLKO.1 701 CDS 100% 6.000 4.200 N PAK3 n/a
5 TRCN0000003245 CCAAACTTCCAACATAACAAA pLKO.1 786 CDS 100% 5.625 3.938 N PAK3 n/a
6 TRCN0000195142 CTTGGAATTATGGCAATTGAA pLKO.1 1822 CDS 100% 5.625 3.938 N PAK3 n/a
7 TRCN0000003246 CTAAGAAGCATTGTGAGTGTT pLKO.1 1243 CDS 100% 4.950 3.465 N PAK3 n/a
8 TRCN0000025158 GCAAAGTAAACGAAGCACTAT pLKO.1 1725 CDS 100% 4.950 3.465 N Pak3 n/a
9 TRCN0000350661 GCAAAGTAAACGAAGCACTAT pLKO_005 1725 CDS 100% 4.950 3.465 N Pak3 n/a
10 TRCN0000003242 GAACAGAAGAAGAACCCACAA pLKO.1 811 CDS 100% 4.050 2.835 N PAK3 n/a
11 TRCN0000003244 GTGGGTGATGAACTATGGGTA pLKO.1 1480 CDS 100% 2.640 1.848 N PAK3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017029559.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487953 TCCTACCTGGTACCGTAATCTGCC pLX_317 18.5% 95.1% 93% V5 (many diffs) n/a
2 TRCN0000488201 GCCAGAGTTGTTACTGTGATCTCG pLX_317 18.7% 95.1% 93% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_14728 pDONR223 99.6% 93.4% 44.4% None (many diffs) n/a
4 ccsbBroad304_14728 pLX_304 0% 93.4% 44.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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