Construct: ORF TRCN0000488201
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020416.1_s317c1
- DNA Barcode:
- GCCAGAGTTGTTACTGTGATCTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PAK3 (5063)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488201
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001128166.2 | 100% | 100% | |
| 2 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001128167.2 | 100% | 100% | |
| 3 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324325.2 | 100% | 100% | |
| 4 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324326.2 | 100% | 100% | |
| 5 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324330.2 | 100% | 100% | |
| 6 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324331.2 | 100% | 100% | |
| 7 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324332.2 | 100% | 100% | |
| 8 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324334.2 | 100% | 100% | |
| 9 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_002578.5 | 100% | 100% | |
| 10 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029561.2 | 100% | 100% | |
| 11 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001128173.3 | 97.3% | 97.3% | 277_321del |
| 12 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324327.2 | 97.3% | 97.3% | 277_321del |
| 13 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324328.2 | 97.3% | 97.3% | 277_321del |
| 14 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324329.2 | 97.3% | 97.3% | 277_321del |
| 15 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324333.2 | 97.3% | 97.3% | 277_321del |
| 16 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029557.1 | 97.3% | 97.3% | 277_321del |
| 17 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001128172.2 | 96.2% | 96.2% | 277_339del |
| 18 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_005262133.3 | 95.1% | 93% | (many diffs) |
| 19 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029558.1 | 95.1% | 93% | (many diffs) |
| 20 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029559.1 | 95.1% | 93% | (many diffs) |
| 21 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029560.1 | 95.1% | 93% | (many diffs) |
| 22 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001128168.3 | 93.7% | 93.7% | 277_384del |
| 23 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029563.1 | 93.7% | 93.7% | 277_384del |
| 24 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_005262132.2 | 92.6% | 90.5% | (many diffs) |
| 25 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_006724654.2 | 92.6% | 90.5% | (many diffs) |
| 26 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_006724655.2 | 92.6% | 90.5% | (many diffs) |
| 27 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_011530962.1 | 92.6% | 90.5% | (many diffs) |
| 28 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_011530967.2 | 92.6% | 90.5% | (many diffs) |
| 29 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_011530968.2 | 92.6% | 90.5% | (many diffs) |
| 30 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_011530969.1 | 92.6% | 90.5% | (many diffs) |
| 31 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029562.1 | 92.1% | 92.1% | (many diffs) |
| 32 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_011530971.2 | 54.8% | 49.2% | (many diffs) |
| 33 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NR_136748.1 | 18.4% | 1_175del;1720_1832del;1921_8837del | |
| 34 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NR_136747.2 | 17.8% | 1_429del;1972_2146del;2237_9153del | |
| 35 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | NM_001195049.1 | 92.3% | 98.5% | (many diffs) |
| 36 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | NM_008778.3 | 92.3% | 98.5% | (many diffs) |
| 37 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_006528753.3 | 92.3% | 98.5% | (many diffs) |
| 38 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_006528754.3 | 92.3% | 98.5% | (many diffs) |
| 39 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_011247790.2 | 92.3% | 98.5% | (many diffs) |
| 40 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | NM_001195048.1 | 89.8% | 95.8% | (many diffs) |
| 41 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_006528752.3 | 89.8% | 95.8% | (many diffs) |
| 42 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318423.1 | 89.8% | 95.8% | (many diffs) |
| 43 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | NM_001195047.1 | 88.9% | 94.8% | (many diffs) |
| 44 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | NM_001195046.1 | 86.6% | 92.4% | (many diffs) |
| 45 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_006528747.3 | 86.6% | 92.4% | (many diffs) |
| 46 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318417.1 | 86.6% | 92.4% | (many diffs) |
| 47 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318418.1 | 86.6% | 92.4% | (many diffs) |
| 48 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318419.1 | 86.6% | 92.4% | (many diffs) |
| 49 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318420.1 | 86.6% | 92.4% | (many diffs) |
| 50 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318421.1 | 86.6% | 92.4% | (many diffs) |
| 51 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318422.1 | 86.6% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 1716
- ORF length:
- 1632
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatgtctg acggtctgga taatgaagag aaacccccgg 121 ctcctccact gaggatgaat agtaacaacc gggattcttc agcactcaac cacagctcca 181 aaccacttcc catggcccct gaagagaaga ataagaaagc caggcttcgc tctatcttcc 241 caggaggagg ggataaaacc aataagaaga aggagaaaga gcgcccagag atctctcttc 301 cttcagactt tgagcatacg attcatgtgg ggtttgatgc agtcaccggg gaattcactg 361 gaattccaga gcaatgggca cgattactcc aaacttccaa cataacaaaa ttggaacaga 421 agaagaaccc acaagctgtt ctagatgttc tcaaattcta tgattccaaa gaaacagtca 481 acaaccagaa atacatgagc tttacatcag gagataaaag tgcacatgga tacatagcag 541 cccatccttc gagtacaaaa acagcatctg agcctccatt ggcccctcct gtgtctgaag 601 aagaagatga agaggaagaa gaagaagaag atgaaaatga gccaccacca gttatcgcac 661 caagaccaga gcatacaaaa tcaatctata ctcgttctgt ggttgaatcc attgcttcac 721 cagcagtacc aaataaagag gtcacaccac cctctgctga aaatgccaat tccagtactt 781 tgtacaggaa cacagatcgg caaagaaaaa aatccaagat gacagatgag gagatcttag 841 agaagctaag aagcattgtg agtgttgggg acccaaagaa aaaatacaca agatttgaaa 901 aaattggtca aggggcatca ggtactgttt atacagcact agacattgca acaggacaag 961 aggtggccat aaagcagatg aaccttcaac agcaacccaa gaaggaatta attattaatg 1021 aaattctggt catgagggaa aataagaacc ctaatattgt taattattta gatagctact 1081 tggtgggtga tgaactatgg gtagtcatgg aatacttggc tggtggctct ctgactgatg 1141 tggtcacaga gacctgtatg gatgaaggac agatagcagc tgtctgcaga gagtgcctgc 1201 aagctttgga tttcctgcac tcaaaccagg tgatccatag agatataaag agtgacaata 1261 ttcttctcgg gatggatggc tctgttaaat tgactgactt tgggttctgt gcccagatca 1321 ctcctgagca aagtaaacga agcactatgg tgggaacccc atattggatg gcacctgagg 1381 tggtgactcg aaaagcttat ggtccgaaag ttgatatctg gtctcttGGA ATTATGGCAA 1441 TTGAAATGGT GGAAGGTGAA CCCCCTTACC TTAATGAAAA TCCACTCAGG GCATTGTATC 1501 TGATAGCCAC TAATGGAACT CCAGAGCTCC AGAATCCTGA GAGACTGTCA GCTGTATTCC 1561 GTGACTTTTT AAATCGCTGT CTTGAGATGG ATGTGGATAG GCGAGGATCT GCCAAGGAGC 1621 TTTTGCAGCA TCCATTTTTA AAATTAGCCA AGCCTCTCTC CAGCCTGACT CCTCTGATTA 1681 TCGCTGCAAA GGAAGCAATT AAGAACAGCA GCCGCTAAAA GGGTGGGCGC GCCGACCCAG 1741 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1801 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1861 TATCTTGTGG AAAGGACGAG CCAGAGTTGT TACTGTGATC TCGACGCGTT AAGTCgacaa 1921 tcaacctctg gattacaaaa tttgtgaaag att