Construct: ORF TRCN0000487953
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019504.1_s317c1
- DNA Barcode:
- TCCTACCTGGTACCGTAATCTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAK3 (5063)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487953
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001128166.2 | 100% | 100% | |
2 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001128167.2 | 100% | 100% | |
3 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324325.2 | 100% | 100% | |
4 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324326.2 | 100% | 100% | |
5 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324330.2 | 100% | 100% | |
6 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324331.2 | 100% | 100% | |
7 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324332.2 | 100% | 100% | |
8 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324334.2 | 100% | 100% | |
9 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_002578.5 | 100% | 100% | |
10 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029561.2 | 100% | 100% | |
11 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001128173.3 | 97.3% | 97.3% | 277_321del |
12 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324327.2 | 97.3% | 97.3% | 277_321del |
13 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324328.2 | 97.3% | 97.3% | 277_321del |
14 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324329.2 | 97.3% | 97.3% | 277_321del |
15 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001324333.2 | 97.3% | 97.3% | 277_321del |
16 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029557.1 | 97.3% | 97.3% | 277_321del |
17 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001128172.2 | 96.2% | 96.2% | 277_339del |
18 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_005262133.3 | 95.1% | 93% | (many diffs) |
19 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029558.1 | 95.1% | 93% | (many diffs) |
20 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029559.1 | 95.1% | 93% | (many diffs) |
21 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029560.1 | 95.1% | 93% | (many diffs) |
22 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NM_001128168.3 | 93.7% | 93.7% | 277_384del |
23 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029563.1 | 93.7% | 93.7% | 277_384del |
24 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_005262132.2 | 92.6% | 90.5% | (many diffs) |
25 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_006724654.2 | 92.6% | 90.5% | (many diffs) |
26 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_006724655.2 | 92.6% | 90.5% | (many diffs) |
27 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_011530962.1 | 92.6% | 90.5% | (many diffs) |
28 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_011530967.2 | 92.6% | 90.5% | (many diffs) |
29 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_011530968.2 | 92.6% | 90.5% | (many diffs) |
30 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_011530969.1 | 92.6% | 90.5% | (many diffs) |
31 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_017029562.1 | 92.1% | 92.1% | (many diffs) |
32 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | XM_011530971.2 | 54.8% | 49.2% | (many diffs) |
33 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NR_136748.1 | 18.4% | 1_175del;1720_1832del;1921_8837del | |
34 | human | 5063 | PAK3 | p21 (RAC1) activated kinase 3 | NR_136747.2 | 17.8% | 1_429del;1972_2146del;2237_9153del | |
35 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | NM_001195049.1 | 92.3% | 98.5% | (many diffs) |
36 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | NM_008778.3 | 92.3% | 98.5% | (many diffs) |
37 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_006528753.3 | 92.3% | 98.5% | (many diffs) |
38 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_006528754.3 | 92.3% | 98.5% | (many diffs) |
39 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_011247790.2 | 92.3% | 98.5% | (many diffs) |
40 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | NM_001195048.1 | 89.8% | 95.8% | (many diffs) |
41 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_006528752.3 | 89.8% | 95.8% | (many diffs) |
42 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318423.1 | 89.8% | 95.8% | (many diffs) |
43 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | NM_001195047.1 | 88.9% | 94.8% | (many diffs) |
44 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | NM_001195046.1 | 86.6% | 92.4% | (many diffs) |
45 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_006528747.3 | 86.6% | 92.4% | (many diffs) |
46 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318417.1 | 86.6% | 92.4% | (many diffs) |
47 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318418.1 | 86.6% | 92.4% | (many diffs) |
48 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318419.1 | 86.6% | 92.4% | (many diffs) |
49 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318420.1 | 86.6% | 92.4% | (many diffs) |
50 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318421.1 | 86.6% | 92.4% | (many diffs) |
51 | mouse | 18481 | Pak3 | p21 protein (Cdc42/Rac)-act... | XM_017318422.1 | 86.6% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 1722
- ORF length:
- 1632
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgga tccctgaaaa tgtctgacgg tctggataat gaagagaaac 121 ccccggctcc tccactgagg atgaatagta acaaccggga ttcttcagca ctcaaccaca 181 gctccaaacc acttcccatg gcccctgaag agaagaataa gaaagccagg cttcgctcta 241 tcttcccagg aggaggggat aaaaccaata agaagaagga gaaagagcgc ccagagatct 301 ctcttccttc agactttgag catacgattc atgtggggtt tgatgcagtc accggggaat 361 tcactggaat tccagagcaa tgggcacgat tactccaaac ttccaacata acaaaattgg 421 aacagaagaa gaacccacaa gctgttctag atgttctcaa attctatgat tccaaagaaa 481 cagtcaacaa ccagaaatac atgagcttta catcaggaga taaaagtgca catggataca 541 tagcagccca tccttcgagt acaaaaacag catctgagcc tccattggcc cctcctgtgt 601 ctgaagaaga agatgaagag gaagaagaag aagaagatga aaatgagcca ccaccagtta 661 tcgcaccaag accagagcat acaaaatcaa tctatactcg ttctgtggtt gaatccattg 721 cttcaccagc agtaccaaat aaagaggtca caccaccctc tgctgaaaat gccaattcca 781 gtactttgta caggaacaca gatcggcaaa gaaaaaaatc caagatgaca gatgaggaga 841 tcttagagaa gctaagaagc attgtgagtg ttggggaccc aaagaaaaaa tacacaagat 901 ttgaaaaaat tggtcaaggg gcatcaggta ctgtttatac agcactagac attgcaacag 961 gacaagaggt ggccataaag cagatgaacc ttcaacagca acccaagaag gaattaatta 1021 ttaatgaaat tctggtcatg agggaaaata agaaccctaa tattgttaat tatttagata 1081 gctacttggt gggtgatgaa ctatgggtag tcatggaata cttggctggt ggctctctga 1141 ctgatgtggt cacagagacc tgtatggatg aaggacagat agcagctgtc tgcagagagt 1201 gcctgcaagc tttggatttc ctgcactcaa accaggtgat ccatagagat ataaagagtg 1261 acaatattct tctcgggatg gatggctctg ttaaattgac tgactttggg ttctgtgccc 1321 agatcactcc tgagcaaagt aaacgaagca ctatggtggg aaccccatat tggatggcac 1381 ctgaggtggt gactcgaaaa gcttatggtc cgaaagttga tatctggtct cttGGAATTA 1441 TGGCAATTGA AATGGTGGAA GGTGAACCCC CTTACCTTAA TGAAAATCCA CTCAGGGCAT 1501 TGTATCTGAT AGCCACTAAT GGAACTCCAG AGCTCCAGAA TCCTGAGAGA CTGTCAGCTG 1561 TATTCCGTGA CTTTTTAAAT CGCTGTCTTG AGATGGATGT GGATAGGCGA GGATCTGCCA 1621 AGGAGCTTTT GCAGCATCCA TTTTTAAAAT TAGCCAAGCC TCTCTCCAGC CTGACTCCTC 1681 TGATTATCGC TGCAAAGGAA GCAATTAAGA ACAGCAGCCG CAAGGGTGGG CGCGCCGACC 1741 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1801 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1861 ATATATCTTG TGGAAAGGAC GATCCTACCT GGTACCGTAA TCTGCCACGC GTTAAGTCga 1921 caatcaacct ctggattaca aaatttgtga aagatt