Transcript: Mouse XM_017313372.1

PREDICTED: Mus musculus zinc finger protein 317 (Zfp317), transcript variant X21, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Zfp317 (244713)
Length:
4998
CDS:
2119..3303

Additional Resources:

NCBI RefSeq record:
XM_017313372.1
NBCI Gene record:
Zfp317 (244713)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017313372.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000434197 GACTGTAGTCAGTGTAGTAAT pLKO_005 2317 CDS 100% 13.200 9.240 N Zfp317 n/a
2 TRCN0000421638 GCAAACTGGCCAGCTCTTTAT pLKO_005 3592 3UTR 100% 13.200 9.240 N Zfp317 n/a
3 TRCN0000416768 TCTAAAGTCAAATGGTCTATT pLKO_005 1888 5UTR 100% 13.200 9.240 N Zfp317 n/a
4 TRCN0000081900 CCTATCAAGAAGGAAACACAT pLKO.1 2688 CDS 100% 4.950 3.465 N Zfp317 n/a
5 TRCN0000081898 CCTGAGTTGTTATGCAGCAAT pLKO.1 3386 3UTR 100% 4.950 3.465 N Zfp317 n/a
6 TRCN0000081902 GCAGATAACCAGGAATCAGAA pLKO.1 1518 5UTR 100% 4.950 3.465 N Zfp317 n/a
7 TRCN0000081899 GCTAGATTCTTCCCAGAGAAA pLKO.1 1694 5UTR 100% 4.950 3.465 N Zfp317 n/a
8 TRCN0000081901 CAATCTAAGTTCACTGGGTTA pLKO.1 1748 5UTR 100% 4.050 2.835 N Zfp317 n/a
9 TRCN0000162177 CAAATGTGATGAGTGTGGCAA pLKO.1 2400 CDS 100% 2.640 1.584 N ZNF28 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017313372.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12390 pDONR223 100% 74.1% 82.6% None (many diffs) n/a
2 ccsbBroad304_12390 pLX_304 0% 74.1% 82.6% V5 (many diffs) n/a
3 TRCN0000473420 AATTGGCTGACAGCTAAGCCGTGT pLX_317 24.3% 74.1% 82.6% V5 (many diffs) n/a
4 ccsbBroadEn_03842 pDONR223 100% 51.6% 57.7% None (many diffs) n/a
5 ccsbBroad304_03842 pLX_304 0% 51.6% 57.7% V5 (many diffs) n/a
6 TRCN0000465801 AGTAAAAAACAGCTTATAAACCTC pLX_317 20% 51.6% 57.7% V5 (many diffs) n/a
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