Transcript: Mouse XM_017318675.1

PREDICTED: Mus musculus UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3galt1), transcript variant X5, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
B3galt1 (26877)
Length:
5518
CDS:
771..1751

Additional Resources:

NCBI RefSeq record:
XM_017318675.1
NBCI Gene record:
B3galt1 (26877)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017318675.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000018787 CCGTTCAGTCACCTAACAGTT pLKO.1 879 CDS 100% 4.950 6.930 N B3galt1 n/a
2 TRCN0000018788 CAAGCCAAGAAGAAGGTATTT pLKO.1 1358 CDS 100% 13.200 9.240 N B3galt1 n/a
3 TRCN0000018789 CAACATAAGAACTCGACCTAT pLKO.1 923 CDS 100% 4.950 3.465 N B3galt1 n/a
4 TRCN0000035800 GCACAGAATCTGGAATGACAT pLKO.1 1703 CDS 100% 4.950 3.465 N B3GALT1 n/a
5 TRCN0000018791 GCATAACCAGACCTACTTCAT pLKO.1 841 CDS 100% 4.950 3.465 N B3galt1 n/a
6 TRCN0000018790 GCCTAGAGATTTGTACCCTGA pLKO.1 1436 CDS 100% 2.160 1.296 N B3galt1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017318675.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01996 pDONR223 100% 90.5% 100% None (many diffs) n/a
2 ccsbBroad304_01996 pLX_304 0% 90.5% 100% V5 (many diffs) n/a
3 TRCN0000477881 AACCAACCCAATCGCGCTCTTCGC pLX_317 37.3% 90.5% 100% V5 (many diffs) n/a
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