Transcript: Mouse XM_017319818.1

PREDICTED: Mus musculus WD repeat domain 47 (Wdr47), transcript variant X6, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Wdr47 (99512)
Length:
4037
CDS:
117..2906

Additional Resources:

NCBI RefSeq record:
XM_017319818.1
NBCI Gene record:
Wdr47 (99512)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017319818.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000216176 CAATAGTCAATGGTAGGTTAA pLKO.1 3621 3UTR 100% 10.800 15.120 N Wdr47 n/a
2 TRCN0000216776 GATTCCAGTCAGAGGTTAATA pLKO.1 1716 CDS 100% 15.000 12.000 N Wdr47 n/a
3 TRCN0000181234 CGTAATGAAGGGCCTACTTTA pLKO.1 728 CDS 100% 13.200 10.560 N Wdr47 n/a
4 TRCN0000178238 GAGCAGGAGATTGTAACATTT pLKO.1 2338 CDS 100% 13.200 10.560 N Wdr47 n/a
5 TRCN0000181888 GCCCTTCTAAAGACGATTACA pLKO.1 520 CDS 100% 5.625 4.500 N Wdr47 n/a
6 TRCN0000198754 CCATACTGTATCACGTGTCTT pLKO.1 3335 3UTR 100% 4.950 3.960 N Wdr47 n/a
7 TRCN0000216743 GCAAGTAACAATCGCTTATTC pLKO.1 702 CDS 100% 13.200 9.240 N Wdr47 n/a
8 TRCN0000217338 GATGGGACAATTCGAGATTTG pLKO.1 2268 CDS 100% 10.800 7.560 N Wdr47 n/a
9 TRCN0000141586 CTTCAGTTCATTCAGCCTCTA pLKO.1 309 CDS 100% 4.050 2.835 N WDR47 n/a
10 TRCN0000182799 CGCTGAGTTTAAGGACTGGAA pLKO.1 584 CDS 100% 2.640 1.848 N Wdr47 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017319818.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07815 pDONR223 100% 89.1% 94.2% None (many diffs) n/a
2 ccsbBroad304_07815 pLX_304 0% 89.1% 94.2% V5 (many diffs) n/a
3 TRCN0000473175 TGCTCAGTTTTTTACCTACCTAGT pLX_317 6.5% 89.1% 94.2% V5 (many diffs) n/a
4 ccsbBroadEn_11648 pDONR223 100% 86.5% 91.4% None (many diffs) n/a
5 ccsbBroad304_11648 pLX_304 0% 86.5% 91.4% V5 (many diffs) n/a
6 TRCN0000471169 GTCCAGGACGTTTATTCGTGAGGT pLX_317 17.7% 86.5% 91.4% V5 (many diffs) n/a
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