Transcript: Mouse XM_017320236.1

PREDICTED: Mus musculus KAT8 regulatory NSL complex subunit 3 (Kansl3), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Kansl3 (226976)
Length:
4697
CDS:
382..2796

Additional Resources:

NCBI RefSeq record:
XM_017320236.1
NBCI Gene record:
Kansl3 (226976)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017320236.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000266994 TGGCTCAGAATAAGCTATTTA pLKO_005 485 CDS 100% 15.000 12.000 N Kansl3 n/a
2 TRCN0000283400 GCAACTCCTGTTCCACTTTAT pLKO_005 337 5UTR 100% 13.200 10.560 N Kansl3 n/a
3 TRCN0000266993 TTCATAGGTCAGAGCATATAA pLKO_005 4022 3UTR 100% 15.000 10.500 N Kansl3 n/a
4 TRCN0000266996 AGCATGGTGGACAGATGTATT pLKO_005 1444 CDS 100% 13.200 9.240 N Kansl3 n/a
5 TRCN0000365610 AGCATGGTGGACAGATGTATT pLKO_005 1444 CDS 100% 13.200 9.240 N KANSL3 n/a
6 TRCN0000217224 CAACCAAGCATCAGGCTTAAA pLKO.1 2628 CDS 100% 13.200 9.240 N Kansl3 n/a
7 TRCN0000266995 CTCCAGTCCTCTTCGTCATTG pLKO_005 1277 CDS 100% 10.800 7.560 N Kansl3 n/a
8 TRCN0000183763 CCTGTTCCACTTTATGACAAT pLKO.1 343 5UTR 100% 4.950 3.465 N Kansl3 n/a
9 TRCN0000179171 GCCTAAGAAGAGCTAGAGAAA pLKO.1 3343 3UTR 100% 4.950 3.465 N Kansl3 n/a
10 TRCN0000183489 GCTTCAAAGTTTGTCTCCTTT pLKO.1 3498 3UTR 100% 4.950 3.465 N Kansl3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017320236.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03629 pDONR223 100% 76.8% 81.1% None (many diffs) n/a
2 ccsbBroad304_03629 pLX_304 0% 76.8% 81.1% V5 (many diffs) n/a
3 TRCN0000470202 ACTTGCCGGATACGATAAGGAAAG pLX_317 15.3% 76.7% 69% V5 (not translated due to prior stop codon) (many diffs) n/a
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