Construct: ORF TRCN0000489363

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020359.1_s317c1
DNA Barcode:
GTTGGTTGTTCTCCAAGACAACGT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
CDKL3 (51265)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489363
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51265 CDKL3 cyclin dependent kinase like 3 NM_016508.3 100% 100%
2 human 51265 CDKL3 cyclin dependent kinase like 3 NM_001113575.2 76.8% 76.8% 1366_1776del
3 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446092.1 76.4% 76.4% 540_575del;1402_1785del
4 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446091.1 76% 76% 540_575del;1402_1794del
5 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009534.1 75.5% 75.5% 540_575del;1402_1806del
6 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446090.1 75.5% 75.5% 540_575del;1402_1806del
7 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009533.1 75.3% 75.3% 540_575del;1402_1812del
8 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009532.1 74.9% 74.9% 1366_1821del
9 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446088.1 74.9% 74.9% 1366_1821del
10 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446089.1 74.9% 74.9% 1366_1821del
11 human 51265 CDKL3 cyclin dependent kinase like 3 XM_011543429.2 73.5% 73.5% 540_575del;1402_1857del
12 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446086.1 73.5% 73.5% 540_575del;1402_1857del
13 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446087.1 73.5% 73.5% 540_575del;1402_1857del
14 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446095.1 72.5% 64% (many diffs)
15 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009536.1 60.4% 60.4% 540_575del;828_829ins243;1159_1614del
16 human 51265 CDKL3 cyclin dependent kinase like 3 XR_002956161.1 52.7% 1_866del;1406_1441del;2267_2587delinsG
17 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446093.1 49.9% 47.7% (many diffs)
18 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446094.1 48.6% 45% (many diffs)
19 human 51265 CDKL3 cyclin dependent kinase like 3 XR_002956160.1 48.4% 1_866del;1406_1441del;2267_2814delinsG
20 human 51265 CDKL3 cyclin dependent kinase like 3 XR_002956159.1 47.4% 1_865del;1405_1440del;2267_2878del
21 human 51265 CDKL3 cyclin dependent kinase like 3 NM_001300853.1 45% 45% 0_1ins567;799_1203del
22 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446103.1 44.9% 44.9% 0_1ins567;799_1209del
23 human 51265 CDKL3 cyclin dependent kinase like 3 NM_001349363.1 43.8% 43.8% 0_1ins567;799_1254del
24 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446096.1 43.8% 43.8% 0_1ins567;799_1254del
25 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446097.1 43.8% 43.8% 0_1ins567;799_1254del
26 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446098.1 43.8% 43.8% 0_1ins567;799_1254del
27 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446099.1 43.8% 43.8% 0_1ins567;799_1254del
28 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446100.1 43.8% 43.8% 0_1ins567;799_1254del
29 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446101.1 43.8% 43.8% 0_1ins567;799_1254del
30 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446102.1 43.8% 43.8% 0_1ins567;799_1254del
31 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446104.1 43.8% 43.8% 0_1ins567;799_1254del
32 human 51265 CDKL3 cyclin dependent kinase like 3 NM_001349364.1 26.3% 26.3% 0_1ins885;481_936del
33 human 51265 CDKL3 cyclin dependent kinase like 3 NM_001349365.1 26.3% 26.3% 0_1ins885;481_936del
34 human 51265 CDKL3 cyclin dependent kinase like 3 NM_001349366.1 26.3% 26.3% 0_1ins885;481_936del
35 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009544.2 26.3% 26.3% 0_1ins885;481_936del
36 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009545.2 26.3% 26.3% 0_1ins885;481_936del
37 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009546.1 26.3% 26.3% 0_1ins885;481_936del
38 human 51265 CDKL3 cyclin dependent kinase like 3 XM_017009547.1 26.3% 26.3% 0_1ins885;481_936del
39 human 51265 CDKL3 cyclin dependent kinase like 3 XM_024446105.1 26.3% 26.3% 0_1ins885;481_936del
40 mouse 213084 Cdkl3 cyclin-dependent kinase-like 3 NM_001166657.1 66.1% 61% (many diffs)
41 mouse 213084 Cdkl3 cyclin-dependent kinase-like 3 XM_017314422.1 66.1% 61% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1437
ORF length:
1365
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggagatg tatgaaaccc ttggaaaagt gggagaggga agttacggaa
121cagtcatgaa atgtaaacat aagaatactg ggcagatagt ggccattaag atattttatg
181agagaccaga acaatctgtc aacaaaattg cgatgagaga aataaagttt ctaaagcaat
241ttcatcacga aaacctggtc aatctgattg aagtttttag acagaaaaag aaaattcatt
301tggtatttga atttattgac cacacagtat tagatgagtt acaacattat tgtcatggac
361tagagagtaa gcgacttaga aaatacctct tccagatcct tcgagcaatt gactatcttc
421acagtaataa tatcattcat cgagatataa aacctgagaa tattttagta tcccagtcag
481gaattactaa gctctgtgat tttggttttg cacgaacact agcagctcct ggggacattt
541atacggacta tgtggccaca cgctggtata gagctcccga attagtatta aaagatactt
601cttatggaaa acctgtggat atctgggctt tgggctgtat gatcattgag atggccactg
661gaaatcccta tcttcctagt agttctgatt tggatttact ccataaaatt gttttgaaag
721tgggcaattt gtcacctcac ttgcagaata tcttttccaa gagccccatt tttgctgggg
781tagttcttcc tcaagttcaa caccccaaaa atgcaagaaa aaaatatcca aagcttaatg
841gattgttggc agatatagtt catgcttgtt tacaaattga tcctgctgac aggatatcat
901ctagtgatct tttgcatcat gagtatttta ctagagatgg atttattgaa aaattcatgc
961cagaactgaa agctaaatta ctgcaggaag caaaagtcaa ttcattaata aagccaaaag
1021agagttcTAA AGAAAATGAA CTCAGGAAAG ATGAAAGAAA AACAGTTTAT ACCAATACAC
1081TGCTAAGTAG TTCAGTTTTG GGAAAGGAAA TAGAAAAAGA GAAAAAGCCC AAGGAGATCA
1141AAGTCAGAGT TATTAAAGTC AAAGGAGGAA GAGGAGATAT CTCAGAACCA AAAAAGAAAG
1201AGTATGAAGG TGGACTTGGT CAACAGGATG CAAATGAAAA TGTTCATCCT ATGTCTCCAG
1261ATACAAAACT TGTAACCATT GAACCACCAA ACCCTATCAA TCCCAGCACT AACTGTAATG
1321GCTTGAAAGA AAATCCACAT TGCGGAGGTT CTGTGACAAT GCCACCCATC AATCTAACTA
1381ACAGTAATTT GATGGCTGCA AATCTCAGTT CAAATCTCTT TCACCCCAGT GTGAGGTAGG
1441ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1501GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1561TTTATATATC TTGTGGAAAG GACGAGTTGG TTGTTCTCCA AGACAACGTA CGCGTTAAGT
1621Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)