Construct: ORF TRCN0000489363
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020359.1_s317c1
- DNA Barcode:
- GTTGGTTGTTCTCCAAGACAACGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CDKL3 (51265)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489363
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_016508.3 | 100% | 100% | |
2 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_001113575.2 | 76.8% | 76.8% | 1366_1776del |
3 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446092.1 | 76.4% | 76.4% | 540_575del;1402_1785del |
4 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446091.1 | 76% | 76% | 540_575del;1402_1794del |
5 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009534.1 | 75.5% | 75.5% | 540_575del;1402_1806del |
6 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446090.1 | 75.5% | 75.5% | 540_575del;1402_1806del |
7 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009533.1 | 75.3% | 75.3% | 540_575del;1402_1812del |
8 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009532.1 | 74.9% | 74.9% | 1366_1821del |
9 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446088.1 | 74.9% | 74.9% | 1366_1821del |
10 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446089.1 | 74.9% | 74.9% | 1366_1821del |
11 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_011543429.2 | 73.5% | 73.5% | 540_575del;1402_1857del |
12 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446086.1 | 73.5% | 73.5% | 540_575del;1402_1857del |
13 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446087.1 | 73.5% | 73.5% | 540_575del;1402_1857del |
14 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446095.1 | 72.5% | 64% | (many diffs) |
15 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009536.1 | 60.4% | 60.4% | 540_575del;828_829ins243;1159_1614del |
16 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XR_002956161.1 | 52.7% | 1_866del;1406_1441del;2267_2587delinsG | |
17 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446093.1 | 49.9% | 47.7% | (many diffs) |
18 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446094.1 | 48.6% | 45% | (many diffs) |
19 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XR_002956160.1 | 48.4% | 1_866del;1406_1441del;2267_2814delinsG | |
20 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XR_002956159.1 | 47.4% | 1_865del;1405_1440del;2267_2878del | |
21 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_001300853.1 | 45% | 45% | 0_1ins567;799_1203del |
22 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446103.1 | 44.9% | 44.9% | 0_1ins567;799_1209del |
23 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_001349363.1 | 43.8% | 43.8% | 0_1ins567;799_1254del |
24 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446096.1 | 43.8% | 43.8% | 0_1ins567;799_1254del |
25 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446097.1 | 43.8% | 43.8% | 0_1ins567;799_1254del |
26 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446098.1 | 43.8% | 43.8% | 0_1ins567;799_1254del |
27 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446099.1 | 43.8% | 43.8% | 0_1ins567;799_1254del |
28 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446100.1 | 43.8% | 43.8% | 0_1ins567;799_1254del |
29 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446101.1 | 43.8% | 43.8% | 0_1ins567;799_1254del |
30 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446102.1 | 43.8% | 43.8% | 0_1ins567;799_1254del |
31 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446104.1 | 43.8% | 43.8% | 0_1ins567;799_1254del |
32 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_001349364.1 | 26.3% | 26.3% | 0_1ins885;481_936del |
33 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_001349365.1 | 26.3% | 26.3% | 0_1ins885;481_936del |
34 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_001349366.1 | 26.3% | 26.3% | 0_1ins885;481_936del |
35 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009544.2 | 26.3% | 26.3% | 0_1ins885;481_936del |
36 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009545.2 | 26.3% | 26.3% | 0_1ins885;481_936del |
37 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009546.1 | 26.3% | 26.3% | 0_1ins885;481_936del |
38 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009547.1 | 26.3% | 26.3% | 0_1ins885;481_936del |
39 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446105.1 | 26.3% | 26.3% | 0_1ins885;481_936del |
40 | mouse | 213084 | Cdkl3 | cyclin-dependent kinase-like 3 | NM_001166657.1 | 66.1% | 61% | (many diffs) |
41 | mouse | 213084 | Cdkl3 | cyclin-dependent kinase-like 3 | XM_017314422.1 | 66.1% | 61% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1437
- ORF length:
- 1365
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagatg tatgaaaccc ttggaaaagt gggagaggga agttacggaa 121 cagtcatgaa atgtaaacat aagaatactg ggcagatagt ggccattaag atattttatg 181 agagaccaga acaatctgtc aacaaaattg cgatgagaga aataaagttt ctaaagcaat 241 ttcatcacga aaacctggtc aatctgattg aagtttttag acagaaaaag aaaattcatt 301 tggtatttga atttattgac cacacagtat tagatgagtt acaacattat tgtcatggac 361 tagagagtaa gcgacttaga aaatacctct tccagatcct tcgagcaatt gactatcttc 421 acagtaataa tatcattcat cgagatataa aacctgagaa tattttagta tcccagtcag 481 gaattactaa gctctgtgat tttggttttg cacgaacact agcagctcct ggggacattt 541 atacggacta tgtggccaca cgctggtata gagctcccga attagtatta aaagatactt 601 cttatggaaa acctgtggat atctgggctt tgggctgtat gatcattgag atggccactg 661 gaaatcccta tcttcctagt agttctgatt tggatttact ccataaaatt gttttgaaag 721 tgggcaattt gtcacctcac ttgcagaata tcttttccaa gagccccatt tttgctgggg 781 tagttcttcc tcaagttcaa caccccaaaa atgcaagaaa aaaatatcca aagcttaatg 841 gattgttggc agatatagtt catgcttgtt tacaaattga tcctgctgac aggatatcat 901 ctagtgatct tttgcatcat gagtatttta ctagagatgg atttattgaa aaattcatgc 961 cagaactgaa agctaaatta ctgcaggaag caaaagtcaa ttcattaata aagccaaaag 1021 agagttcTAA AGAAAATGAA CTCAGGAAAG ATGAAAGAAA AACAGTTTAT ACCAATACAC 1081 TGCTAAGTAG TTCAGTTTTG GGAAAGGAAA TAGAAAAAGA GAAAAAGCCC AAGGAGATCA 1141 AAGTCAGAGT TATTAAAGTC AAAGGAGGAA GAGGAGATAT CTCAGAACCA AAAAAGAAAG 1201 AGTATGAAGG TGGACTTGGT CAACAGGATG CAAATGAAAA TGTTCATCCT ATGTCTCCAG 1261 ATACAAAACT TGTAACCATT GAACCACCAA ACCCTATCAA TCCCAGCACT AACTGTAATG 1321 GCTTGAAAGA AAATCCACAT TGCGGAGGTT CTGTGACAAT GCCACCCATC AATCTAACTA 1381 ACAGTAATTT GATGGCTGCA AATCTCAGTT CAAATCTCTT TCACCCCAGT GTGAGGTAGG 1441 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1501 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1561 TTTATATATC TTGTGGAAAG GACGAGTTGG TTGTTCTCCA AGACAACGTA CGCGTTAAGT 1621 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt