Construct: ORF TRCN0000489746
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019673.1_s317c1
- DNA Barcode:
- AGCGACCGACGTAAGGTACTCGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDKL3 (51265)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489746
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_016508.3 | 99.9% | 99.7% | 1365_1366insG |
2 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_001113575.2 | 76.8% | 76.8% | 1366_1776delinsG |
3 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446092.1 | 76.4% | 76.4% | 540_575del;1402_1785delinsG |
4 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446091.1 | 76% | 76% | 540_575del;1402_1794delinsG |
5 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009534.1 | 75.5% | 75.5% | 540_575del;1402_1806delinsG |
6 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446090.1 | 75.5% | 75.5% | 540_575del;1402_1806delinsG |
7 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009533.1 | 75.3% | 75.3% | 540_575del;1402_1812delinsG |
8 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009532.1 | 74.9% | 74.9% | 1366_1821delinsG |
9 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446088.1 | 74.9% | 74.9% | 1366_1821delinsG |
10 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446089.1 | 74.9% | 74.9% | 1366_1821delinsG |
11 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_011543429.2 | 73.5% | 73.5% | 540_575del;1402_1857delinsG |
12 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446086.1 | 73.5% | 73.5% | 540_575del;1402_1857delinsG |
13 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446087.1 | 73.5% | 73.5% | 540_575del;1402_1857delinsG |
14 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446095.1 | 72.6% | 63.9% | (many diffs) |
15 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009536.1 | 60.4% | 60.4% | 540_575del;828_829ins243;1159_1614delinsG |
16 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XR_002956161.1 | 52.7% | (many diffs) | |
17 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446093.1 | 49.9% | 47.7% | (many diffs) |
18 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446094.1 | 48.6% | 45% | (many diffs) |
19 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XR_002956160.1 | 48.5% | (many diffs) | |
20 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XR_002956159.1 | 47.4% | 1_865del;1405_1440del;2267_2878delinsG | |
21 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_001300853.1 | 45% | 45% | 0_1ins567;799_1203delinsG |
22 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446103.1 | 44.9% | 44.9% | 0_1ins567;799_1209delinsG |
23 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_001349363.1 | 43.8% | 43.8% | 0_1ins567;799_1254delinsG |
24 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446096.1 | 43.8% | 43.8% | 0_1ins567;799_1254delinsG |
25 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446097.1 | 43.8% | 43.8% | 0_1ins567;799_1254delinsG |
26 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446098.1 | 43.8% | 43.8% | 0_1ins567;799_1254delinsG |
27 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446099.1 | 43.8% | 43.8% | 0_1ins567;799_1254delinsG |
28 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446100.1 | 43.8% | 43.8% | 0_1ins567;799_1254delinsG |
29 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446101.1 | 43.8% | 43.8% | 0_1ins567;799_1254delinsG |
30 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446102.1 | 43.8% | 43.8% | 0_1ins567;799_1254delinsG |
31 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446104.1 | 43.8% | 43.8% | 0_1ins567;799_1254delinsG |
32 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_001349364.1 | 26.3% | 26.3% | 0_1ins885;481_936delinsG |
33 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_001349365.1 | 26.3% | 26.3% | 0_1ins885;481_936delinsG |
34 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | NM_001349366.1 | 26.3% | 26.3% | 0_1ins885;481_936delinsG |
35 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009544.2 | 26.3% | 26.3% | 0_1ins885;481_936delinsG |
36 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009545.2 | 26.3% | 26.3% | 0_1ins885;481_936delinsG |
37 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009546.1 | 26.3% | 26.3% | 0_1ins885;481_936delinsG |
38 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_017009547.1 | 26.3% | 26.3% | 0_1ins885;481_936delinsG |
39 | human | 51265 | CDKL3 | cyclin dependent kinase like 3 | XM_024446105.1 | 26.3% | 26.3% | 0_1ins885;481_936delinsG |
40 | mouse | 213084 | Cdkl3 | cyclin-dependent kinase-like 3 | NM_001166657.1 | 66% | 60.9% | (many diffs) |
41 | mouse | 213084 | Cdkl3 | cyclin-dependent kinase-like 3 | XM_017314422.1 | 66% | 60.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1443
- ORF length:
- 1368
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag atgtatgaaa cccttggaaa agtgggagag ggaagttacg 121 gaacagtcat gaaatgtaaa cataagaata ctgggcagat agtggccatt aagatatttt 181 atgagagacc agaacaatct gtcaacaaaa ttgcgatgag agaaataaag tttctaaagc 241 aatttcatca cgaaaacctg gtcaatctga ttgaagtttt tagacagaaa aagaaaattc 301 atttggtatt tgaatttatt gaccacacag tattagatga gttacaacat tattgtcatg 361 gactagagag taagcgactt agaaaatacc tcttccagat ccttcgagca attgactatc 421 ttcacagtaa taatatcatt catcgagata taaaacctga gaatatttta gtatcccagt 481 caggaattac taagctctgt gattttggtt ttgcacgaac actagcagct cctggggaca 541 tttatacgga ctatgtggcc acacgctggt atagagctcc cgaattagta ttaaaagata 601 cttcttatgg aaaacctgtg gatatctggg ctttgggctg tatgatcatt gagatggcca 661 ctggaaatcc ctatcttcct agtagttctg atttggattt actccataaa attgttttga 721 aagtgggcaa tttgtcacct cacttgcaga atatcttttc caagagcccc atttttgctg 781 gggtagttct tcctcaagtt caacacccca aaaatgcaag aaaaaaatat ccaaagctta 841 atggattgtt ggcagatata gttcatgctt gtttacaaat tgatcctgct gacaggatat 901 catctagtga tcttttgcat catgagtatt ttactagaga tggatttatt gaaaaattca 961 tgccagaact gaaagctaaa ttactgcagg aagcaaaagt caattcatta ataaagccaa 1021 aagagagttc taaagaaaat gaactcagga aagatgaaag aaaaaCAGTT TATACCAATA 1081 CACTGCTAAG TAGTTCAGTT TTGGGAAAGG AAATAGAAAA AGAGAAAAAG CCCAAGGAGA 1141 TCAAAGTCAG AGTTATTAAA GTCAAAGGAG GAAGAGGAGA TATCTCAGAA CCAAAAAAGA 1201 AAGAGTATGA AGGTGGACTT GGTCAACAGG ATGCAAATGA AAATGTTCAT CCTATGTCTC 1261 CAGATACAAA ACTTGTAACC ATTGAACCAC CAAACCCTAT CAATCCCAGC ACTAACTGTA 1321 ATGGCTTGAA AGAAAATCCA CATTGCGGAG GTTCTGTGAC AATGCCACCC ATCAATCTAA 1381 CTAACAGTAA TTTGATGGCT GCAAATCTCA GTTCAAATCT CTTTCACCCC AGTGTGAGGG 1441 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1501 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1561 TTTATATATC TTGTGGAAAG GACGAAGCGA CCGACGTAAG GTACTCGAGA CGCGTTAAGT 1621 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt