Transcript: Human XM_024448260.1

PREDICTED: Homo sapiens zinc finger AN1-type containing 4 (ZFAND4), transcript variant X22, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZFAND4 (93550)
Length:
4503
CDS:
787..2634

Additional Resources:

NCBI RefSeq record:
XM_024448260.1
NBCI Gene record:
ZFAND4 (93550)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024448260.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000007776 GCCATCTAGTAATGGGAACAT pLKO.1 1572 CDS 100% 4.950 6.930 N ZFAND4 n/a
2 TRCN0000007775 GCGGACCTATAAATACTAGAA pLKO.1 597 5UTR 100% 4.950 6.930 N ZFAND4 n/a
3 TRCN0000007773 CGGACATGAATCCATTCTATA pLKO.1 3245 3UTR 100% 13.200 10.560 N ZFAND4 n/a
4 TRCN0000007774 CCGGGAATTAAGTCCTCACAA pLKO.1 1833 CDS 100% 4.950 3.960 N ZFAND4 n/a
5 TRCN0000007777 GCCTGTTGGTTGTGTAAATAA pLKO.1 2127 CDS 100% 15.000 10.500 N ZFAND4 n/a
6 TRCN0000432629 ACTTGCTTTCAAGGTGTTAAA pLKO_005 2020 CDS 100% 13.200 9.240 N ZFAND4 n/a
7 TRCN0000421670 TCCTACAGACGATCCACTTAG pLKO_005 762 5UTR 100% 10.800 7.560 N ZFAND4 n/a
8 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 694 5UTR 100% 5.625 2.813 Y KLHL30 n/a
9 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 694 5UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024448260.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12996 pDONR223 100% 92.3% 92.4% None 0_1ins132;214_231del;1263A>G n/a
2 ccsbBroad304_12996 pLX_304 0% 92.3% 92.4% V5 0_1ins132;214_231del;1263A>G n/a
3 TRCN0000479053 ACGTTTCACCATTGGGCGGATGGT pLX_317 19.6% 92.3% 92.4% V5 0_1ins132;214_231del;1263A>G n/a
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