Transcript: Human XM_024448324.1

PREDICTED: Homo sapiens T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 (TCIRG1), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TCIRG1 (10312)
Length:
2219
CDS:
122..2125

Additional Resources:

NCBI RefSeq record:
XM_024448324.1
NBCI Gene record:
TCIRG1 (10312)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024448324.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000038634 AGACGCTTTGTGGTTGATGTT pLKO.1 266 CDS 100% 4.950 3.465 N TCIRG1 n/a
2 TRCN0000289222 AGACGCTTTGTGGTTGATGTT pLKO_005 266 CDS 100% 4.950 3.465 N TCIRG1 n/a
3 TRCN0000038636 CCTCGTGTTCCTAGTCATCTA pLKO.1 1963 CDS 100% 4.950 3.465 N TCIRG1 n/a
4 TRCN0000289300 CCTCGTGTTCCTAGTCATCTA pLKO_005 1963 CDS 100% 4.950 3.465 N TCIRG1 n/a
5 TRCN0000038637 CCACAAGATGAAGGCCGTGTA pLKO.1 1025 CDS 100% 4.050 2.835 N TCIRG1 n/a
6 TRCN0000307047 CCACAAGATGAAGGCCGTGTA pLKO_005 1025 CDS 100% 4.050 2.835 N TCIRG1 n/a
7 TRCN0000038638 CAACTCCTTCAAGATGAAGAT pLKO.1 1804 CDS 100% 4.950 2.970 N TCIRG1 n/a
8 TRCN0000289223 CAACTCCTTCAAGATGAAGAT pLKO_005 1804 CDS 100% 4.950 2.970 N TCIRG1 n/a
9 TRCN0000101699 GAGTTCAGAGACCTCAACGAA pLKO.1 227 CDS 100% 3.000 2.100 N Tcirg1 n/a
10 TRCN0000038635 CCGCAAGATCACGGACTGCTT pLKO.1 817 CDS 100% 0.880 0.616 N TCIRG1 n/a
11 TRCN0000289299 CCGCAAGATCACGGACTGCTT pLKO_005 817 CDS 100% 0.880 0.616 N TCIRG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024448324.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02396 pDONR223 100% 73.5% 46.1% None (many diffs) n/a
2 ccsbBroad304_02396 pLX_304 0% 73.5% 46.1% V5 (many diffs) n/a
3 TRCN0000471826 TCCGTTCCGAGGCATCACTTCAGT pLX_317 14.9% 73.5% 46.1% V5 (many diffs) n/a
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