Transcript: Human XM_024449051.1

PREDICTED: Homo sapiens limb development membrane protein 1 like (LMBR1L), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
LMBR1L (55716)
Length:
2746
CDS:
335..1840

Additional Resources:

NCBI RefSeq record:
XM_024449051.1
NBCI Gene record:
LMBR1L (55716)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024449051.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000236314 ATAGCCATGTTTACATGATTT pLKO_005 2139 3UTR 100% 13.200 18.480 N LMBR1L n/a
2 TRCN0000236315 GAGAGGATCCGCGAGTGTATT pLKO_005 386 CDS 100% 13.200 18.480 N LMBR1L n/a
3 TRCN0000063472 ACTGCCATGACGCAGATAATT pLKO.1 1529 CDS 100% 15.000 10.500 N LMBR1L n/a
4 TRCN0000236312 GCACCTTTACCCTGGCAATTG pLKO_005 537 CDS 100% 10.800 7.560 N LMBR1L n/a
5 TRCN0000126109 GCCATGTTTACATGATTTGAT pLKO.1 2142 3UTR 100% 5.625 3.938 N Lmbr1l n/a
6 TRCN0000353810 GCCATGTTTACATGATTTGAT pLKO_005 2142 3UTR 100% 5.625 3.938 N Lmbr1l n/a
7 TRCN0000063468 GCCAACAGAGAGTCACTCTAT pLKO.1 875 CDS 100% 4.950 3.465 N LMBR1L n/a
8 TRCN0000063470 CCTTTAGACATGGAGCTGCTA pLKO.1 1124 CDS 100% 2.640 1.848 N LMBR1L n/a
9 TRCN0000063469 GCAGGATCTGTAATCCTACTT pLKO.1 1092 CDS 100% 0.495 0.347 N LMBR1L n/a
10 TRCN0000236316 ACACTGCCATGACGCAGATAA pLKO_005 1527 CDS 100% 13.200 7.920 N LMBR1L n/a
11 TRCN0000236313 TTCAACTGGCTGGGCAATTTC pLKO_005 1649 CDS 100% 13.200 7.920 N LMBR1L n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024449051.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12258 pDONR223 100% 93.5% 93.4% None 631G>T;853_948del n/a
2 ccsbBroad304_12258 pLX_304 0% 93.5% 93.4% V5 631G>T;853_948del n/a
3 TRCN0000480182 TGTGATTCGCAGACCTGGGCTGCC pLX_317 23.8% 93.5% 93.4% V5 631G>T;853_948del n/a
4 ccsbBroadEn_15910 pDONR223 0% 90% 90% None 853_948del;1101_1102ins60 n/a
5 ccsbBroad304_15910 pLX_304 0% 90% 90% V5 853_948del;1101_1102ins60 n/a
6 TRCN0000469583 TTCAGCGCCCAATACCCGCTGGTA pLX_317 29.9% 90% 90% V5 853_948del;1101_1102ins60 n/a
7 ccsbBroadEn_08568 pDONR223 100% 89.9% 90% None 853_948del;1038G>T;1101_1102ins60 n/a
8 ccsbBroad304_08568 pLX_304 0% 89.9% 90% V5 853_948del;1038G>T;1101_1102ins60 n/a
9 TRCN0000478741 TACAGCTGCTGTGGGTCAATCCGC pLX_317 18.4% 89.9% 90% V5 853_948del;1038G>T;1101_1102ins60 n/a
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