Construct: ORF ccsbBroad304_12258
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010179.1_s304c1, BRDN0000411542
- Derived from:
- ccsbBroadEn_12258
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMBR1L (55716)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12258
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55716 | LMBR1L | limb development membrane p... | NM_001300751.1 | 99.9% | 99.7% | 631G>T |
| 2 | human | 55716 | LMBR1L | limb development membrane p... | NM_018113.3 | 95.8% | 95.7% | 631G>T;1006_1065del |
| 3 | human | 55716 | LMBR1L | limb development membrane p... | NM_001352161.1 | 94.4% | 94.3% | 631G>T;1021_1101del |
| 4 | human | 55716 | LMBR1L | limb development membrane p... | XM_024449051.1 | 93.5% | 93.4% | 631G>T;853_948del |
| 5 | human | 55716 | LMBR1L | limb development membrane p... | XM_017019627.2 | 92.6% | 88.1% | (many diffs) |
| 6 | human | 55716 | LMBR1L | limb development membrane p... | XM_024449047.1 | 89.9% | 89.8% | 631G>T;853_948del;1102_1161del |
| 7 | human | 55716 | LMBR1L | limb development membrane p... | NM_001300750.1 | 88.9% | 84.6% | (many diffs) |
| 8 | human | 55716 | LMBR1L | limb development membrane p... | XM_017019626.2 | 88.9% | 84.6% | (many diffs) |
| 9 | human | 55716 | LMBR1L | limb development membrane p... | XM_024449053.1 | 88.4% | 88.2% | 631G>T;1179_1180ins162 |
| 10 | human | 55716 | LMBR1L | limb development membrane p... | NM_001352162.1 | 83.9% | 83.8% | 156_157ins174;457G>T;832_891del |
| 11 | human | 55716 | LMBR1L | limb development membrane p... | XM_024449048.1 | 83.5% | 79.5% | (many diffs) |
| 12 | human | 55716 | LMBR1L | limb development membrane p... | XM_024449049.1 | 83.5% | 79.5% | (many diffs) |
| 13 | human | 55716 | LMBR1L | limb development membrane p... | XM_024449050.1 | 83.5% | 79.5% | (many diffs) |
| 14 | human | 55716 | LMBR1L | limb development membrane p... | XM_024449052.1 | 79.5% | 79.4% | (many diffs) |
| 15 | human | 55716 | LMBR1L | limb development membrane p... | NM_001352165.1 | 69.8% | 69.7% | 0_1ins381;250G>T;625_684del |
| 16 | human | 55716 | LMBR1L | limb development membrane p... | NM_001352166.1 | 69.8% | 69.7% | 0_1ins381;250G>T;625_684del |
| 17 | human | 55716 | LMBR1L | limb development membrane p... | NM_001352163.1 | 68.8% | 68.7% | 0_1ins381;250G>T;640_720del |
| 18 | human | 55716 | LMBR1L | limb development membrane p... | XM_024449054.1 | 68.2% | 67% | (many diffs) |
| 19 | human | 55716 | LMBR1L | limb development membrane p... | NM_001352164.1 | 61.3% | 61.1% | 0_1ins381;250G>T;798_799ins162 |
| 20 | human | 55716 | LMBR1L | limb development membrane p... | XM_017019631.2 | 55.6% | 49.4% | 0_1ins559;70_71ins65 |
| 21 | human | 55716 | LMBR1L | limb development membrane p... | NM_001352167.1 | 53.3% | 47.5% | 0_1ins559;70_71ins65;382_441del |
| 22 | human | 55716 | LMBR1L | limb development membrane p... | NM_001352168.1 | 53.3% | 47.5% | 0_1ins559;70_71ins65;382_441del |
| 23 | human | 55716 | LMBR1L | limb development membrane p... | XM_017019630.2 | 53.3% | 47.5% | 0_1ins559;70_71ins65;382_441del |
| 24 | human | 55716 | LMBR1L | limb development membrane p... | XR_245944.3 | 48.7% | (many diffs) | |
| 25 | human | 55716 | LMBR1L | limb development membrane p... | XR_002957350.1 | 47.1% | (many diffs) | |
| 26 | human | 55716 | LMBR1L | limb development membrane p... | XR_001748802.1 | 41.1% | (many diffs) | |
| 27 | human | 55716 | LMBR1L | limb development membrane p... | XR_001748803.2 | 33.3% | (many diffs) | |
| 28 | mouse | 74775 | Lmbr1l | limb region 1 like | NM_029098.3 | 86.2% | 92.2% | (many diffs) |
| 29 | mouse | 74775 | Lmbr1l | limb region 1 like | XM_011245758.2 | 77.5% | 79.2% | (many diffs) |
| 30 | mouse | 74775 | Lmbr1l | limb region 1 like | XM_017316773.1 | 74.5% | 75.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1473
- ORF length:
- 1407
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga agcacctgac tacgaagtgc tatccgtgcg agaacagcta ttccacgaga 121 ggatccgcga gtgtattata tcaacacttc tgtttgcaac actgtacatc ctctgccaca 181 tcttcctgac ccgcttcaag aagcctgctg agttcaccac agtggatgat gaagatgcca 241 ccgtcaacaa gattgcgctc gagctgtgca cctttaccct ggcaattgcc ctgggtgctg 301 tcctgctcct gcccttctcc atcatcagca atgaggtgct gctctccctg cctcggaact 361 actacatcca gtggctcaac ggctccctca tccatggcct ctggaacctt gtttttctct 421 tctccaacct gtccctcatc ttcctcatgc cctttgcata tttcttcact gagtctgagg 481 gctttgctgg ctccagaaag ggtgtcctgg gccgggtcta tgagacagtg gtgatgttga 541 tgctcctcac tctgctggtg ctaggtatgg tgtgggtggc atcagccatt gtggacaaga 601 acaaggccaa cagagagtca ctctatgact tttgggagta ctatctcccc tacctctact 661 catgcatctc cttccttggg gttctgctgc tcctgttgtg tactccactg ggtctcgccc 721 gcatgttctc cgtcactggg aagctgctag tcaagccccg gctgctggaa gacctggagg 781 agcagctgta ctgctcagcc tttgaggagg cagccctgac ccgcaggatc tgtaatccta 841 cttcctgctg gctgccttta gacatggagc tgctacacag acaggtcctg gctctgcaga 901 cacagagggt cctgctggag aagaggcgga aggcttcagc ctggcaacgg aacctgggct 961 accccctggc tatgctgtgc ttgctggtgc tgacgggcct gtctgtgctc attgtggcca 1021 tccacatcct ggagctgctc atcgatgagg ctgccatgcc ccgaggcatg caggttgtac 1081 tcatctttta cctaatggtg tcctcagttg tgggcttcta tagctctcca ctcttccgga 1141 gcctgcggcc cagatggcac gacactgcca tgacgcagat aattgggaac tgtgtctgtc 1201 tcctggtcct aagctcagca cttcctgtct tctctcgaac cctggggctc actcgctttg 1261 acctgctggg tgactttgga cgcttcaact ggctgggcaa tttctacatt gtgttcctct 1321 acaacgcagc ctttgcaggc ctcaccacac tctgtctggt gaagaccttc actgcagctg 1381 tgcgggcaga gctgatccgg gcctttgggc tggacagact gccgctgccc gtctccggtt 1441 tcccccaggc atctaggaag acccagcacc agtgcccaac tttcttgtac aaagtggttg 1501 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1561 accggtggcg cgttaagtcg acaatcaacc tctggatta