Construct: ORF TRCN0000480182

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010179.1_s317c1
Derived from:
ccsbBroadEn_12258
DNA Barcode:
TGTGATTCGCAGACCTGGGCTGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LMBR1L (55716)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480182
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55716 LMBR1L limb development membrane p... NM_001300751.1 99.9% 99.7% 631G>T
2 human 55716 LMBR1L limb development membrane p... NM_018113.3 95.8% 95.7% 631G>T;1006_1065del
3 human 55716 LMBR1L limb development membrane p... NM_001352161.1 94.4% 94.3% 631G>T;1021_1101del
4 human 55716 LMBR1L limb development membrane p... XM_024449051.1 93.5% 93.4% 631G>T;853_948del
5 human 55716 LMBR1L limb development membrane p... XM_017019627.2 92.6% 88.1% (many diffs)
6 human 55716 LMBR1L limb development membrane p... XM_024449047.1 89.9% 89.8% 631G>T;853_948del;1102_1161del
7 human 55716 LMBR1L limb development membrane p... NM_001300750.1 88.9% 84.6% (many diffs)
8 human 55716 LMBR1L limb development membrane p... XM_017019626.2 88.9% 84.6% (many diffs)
9 human 55716 LMBR1L limb development membrane p... XM_024449053.1 88.4% 88.2% 631G>T;1179_1180ins162
10 human 55716 LMBR1L limb development membrane p... NM_001352162.1 83.9% 83.8% 156_157ins174;457G>T;832_891del
11 human 55716 LMBR1L limb development membrane p... XM_024449048.1 83.5% 79.5% (many diffs)
12 human 55716 LMBR1L limb development membrane p... XM_024449049.1 83.5% 79.5% (many diffs)
13 human 55716 LMBR1L limb development membrane p... XM_024449050.1 83.5% 79.5% (many diffs)
14 human 55716 LMBR1L limb development membrane p... XM_024449052.1 79.5% 79.4% (many diffs)
15 human 55716 LMBR1L limb development membrane p... NM_001352165.1 69.8% 69.7% 0_1ins381;250G>T;625_684del
16 human 55716 LMBR1L limb development membrane p... NM_001352166.1 69.8% 69.7% 0_1ins381;250G>T;625_684del
17 human 55716 LMBR1L limb development membrane p... NM_001352163.1 68.8% 68.7% 0_1ins381;250G>T;640_720del
18 human 55716 LMBR1L limb development membrane p... XM_024449054.1 68.2% 67% (many diffs)
19 human 55716 LMBR1L limb development membrane p... NM_001352164.1 61.3% 61.1% 0_1ins381;250G>T;798_799ins162
20 human 55716 LMBR1L limb development membrane p... XM_017019631.2 55.6% 49.4% 0_1ins559;70_71ins65
21 human 55716 LMBR1L limb development membrane p... NM_001352167.1 53.3% 47.5% 0_1ins559;70_71ins65;382_441del
22 human 55716 LMBR1L limb development membrane p... NM_001352168.1 53.3% 47.5% 0_1ins559;70_71ins65;382_441del
23 human 55716 LMBR1L limb development membrane p... XM_017019630.2 53.3% 47.5% 0_1ins559;70_71ins65;382_441del
24 human 55716 LMBR1L limb development membrane p... XR_245944.3 48.7% (many diffs)
25 human 55716 LMBR1L limb development membrane p... XR_002957350.1 47.1% (many diffs)
26 human 55716 LMBR1L limb development membrane p... XR_001748802.1 41.1% (many diffs)
27 human 55716 LMBR1L limb development membrane p... XR_001748803.2 33.3% (many diffs)
28 mouse 74775 Lmbr1l limb region 1 like NM_029098.3 86.2% 92.2% (many diffs)
29 mouse 74775 Lmbr1l limb region 1 like XM_011245758.2 77.5% 79.2% (many diffs)
30 mouse 74775 Lmbr1l limb region 1 like XM_017316773.1 74.5% 75.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1473
ORF length:
1407
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga agcacctgac tacgaagtgc tatccgtgcg agaacagcta ttccacgaga
121ggatccgcga gtgtattata tcaacacttc tgtttgcaac actgtacatc ctctgccaca
181tcttcctgac ccgcttcaag aagcctgctg agttcaccac agtggatgat gaagatgcca
241ccgtcaacaa gattgcgctc gagctgtgca cctttaccct ggcaattgcc ctgggtgctg
301tcctgctcct gcccttctcc atcatcagca atgaggtgct gctctccctg cctcggaact
361actacatcca gtggctcaac ggctccctca tccatggcct ctggaacctt gtttttctct
421tctccaacct gtccctcatc ttcctcatgc cctttgcata tttcttcact gagtctgagg
481gctttgctgg ctccagaaag ggtgtcctgg gccgggtcta tgagacagtg gtgatgttga
541tgctcctcac tctgctggtg ctaggtatgg tgtgggtggc atcagccatt gtggacaaga
601acaaggccaa cagagagtca ctctatgact tttgggagta ctatctcccc tacctctact
661catgcatctc cttccttggg gttctgctgc tcctgttgtg tactccactg ggtctcgccc
721gcatgttctc cgtcactggg aagctgctag tcaagccccg gctgctggaa gacctggagg
781agcagctgta ctgctcagcc tttgaggagg cagccctgac ccgcaggatc tgtaatccta
841cttcctgctg gctgccttta gacatggagc tgctacacag acaggtcctg gctctgcaga
901cacagagggt cctgctggag aagaggcgga aggcttcagc ctggcaacgg aacctgggct
961accccctggc tatgctgtgc ttgctggtgc tgacgggcct gtctgtgctc attgtggcca
1021tccacatcct ggagctgctc atcgatgagg ctgccatgcc ccgaggcatg caggttgtac
1081tcatctttta cctaatggtg tcctcagttg tgggcttcta tagctctcca ctcttccgga
1141gccTGCGGCC CAGATGGCAC GACACTGCCA TGACGCAGAT AATTGGGAAC TGTGTCTGTC
1201TCCTGGTCCT AAGCTCAGCA CTTCCTGTCT TCTCTCGAAC CCTGGGGCTC ACTCGCTTTG
1261ACCTGCTGGG TGACTTTGGA CGCTTCAACT GGCTGGGCAA TTTCTACATT GTGTTCCTCT
1321ACAACGCAGC CTTTGCAGGC CTCACCACAC TCTGTCTGGT GAAGACCTTC ACTGCAGCTG
1381TGCGGGCAGA GCTGATCCGG GCCTTTGGGC TGGACAGACT GCCGCTGCCC GTCTCCGGTT
1441TCCCCCAGGC ATCTAGGAAG ACCCAGCACC AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG
1501ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1561GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG
1621TGATTCGCAG ACCTGGGCTG CCACGCGTTA AGTCgacaat caacctctgg attacaaaat
1681ttgtgaaaga tt

Download FASTA (ORF) (Full)