Construct: ORF ccsbBroad304_08568

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005681.1_s304c1, BRDN0000399147
Derived from:
ccsbBroadEn_08568
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LMBR1L (55716)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08568
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55716 LMBR1L limb development membrane p... NM_018113.3 99.9% 100% 942G>T
2 human 55716 LMBR1L limb development membrane p... NM_001300751.1 95.8% 95.9% 942G>T;1005_1006ins60
3 human 55716 LMBR1L limb development membrane p... NM_001352161.1 95.5% 94.9% (many diffs)
4 human 55716 LMBR1L limb development membrane p... XM_024449047.1 93.7% 93.8% 853_948del;1038G>T
5 human 55716 LMBR1L limb development membrane p... NM_001300750.1 92.9% 88.8% (many diffs)
6 human 55716 LMBR1L limb development membrane p... XM_017019626.2 92.9% 88.8% (many diffs)
7 human 55716 LMBR1L limb development membrane p... XM_024449051.1 89.9% 90% 853_948del;1038G>T;1101_1102ins60
8 human 55716 LMBR1L limb development membrane p... XM_017019627.2 88.9% 84.8% (many diffs)
9 human 55716 LMBR1L limb development membrane p... NM_001352162.1 88% 88.1% 156_157ins174;768G>T
10 human 55716 LMBR1L limb development membrane p... XM_024449048.1 87.3% 83.4% (many diffs)
11 human 55716 LMBR1L limb development membrane p... XM_024449049.1 87.3% 83.4% (many diffs)
12 human 55716 LMBR1L limb development membrane p... XM_024449050.1 87.3% 83.4% (many diffs)
13 human 55716 LMBR1L limb development membrane p... XM_024449053.1 84.7% 84.8% 942G>T;1005_1006ins60;1179_1180ins162
14 human 55716 LMBR1L limb development membrane p... XM_024449052.1 83.4% 83.4% 853_948del;1038G>T;1335_1336ins162
15 human 55716 LMBR1L limb development membrane p... NM_001352165.1 73.9% 74% 0_1ins381;561G>T
16 human 55716 LMBR1L limb development membrane p... NM_001352166.1 73.9% 74% 0_1ins381;561G>T
17 human 55716 LMBR1L limb development membrane p... NM_001352163.1 70.2% 69.3% (many diffs)
18 human 55716 LMBR1L limb development membrane p... XM_024449054.1 65.4% 64.8% (many diffs)
19 human 55716 LMBR1L limb development membrane p... NM_001352164.1 58.8% 58.8% (many diffs)
20 human 55716 LMBR1L limb development membrane p... NM_001352167.1 57.3% 51.4% 0_1ins559;70_71ins65;318G>T
21 human 55716 LMBR1L limb development membrane p... NM_001352168.1 57.3% 51.4% 0_1ins559;70_71ins65;318G>T
22 human 55716 LMBR1L limb development membrane p... XM_017019630.2 57.3% 51.4% 0_1ins559;70_71ins65;318G>T
23 human 55716 LMBR1L limb development membrane p... XM_017019631.2 53.3% 47.5% (many diffs)
24 human 55716 LMBR1L limb development membrane p... XR_245944.3 48.1% (many diffs)
25 human 55716 LMBR1L limb development membrane p... XR_002957350.1 46.6% (many diffs)
26 human 55716 LMBR1L limb development membrane p... XR_001748802.1 42.7% (many diffs)
27 human 55716 LMBR1L limb development membrane p... XR_001748803.2 34.6% (many diffs)
28 mouse 74775 Lmbr1l limb region 1 like NM_029098.3 89.7% 95.5% (many diffs)
29 mouse 74775 Lmbr1l limb region 1 like XM_011245758.2 80.5% 82.2% (many diffs)
30 mouse 74775 Lmbr1l limb region 1 like XM_017316773.1 77.5% 78.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1533
ORF length:
1467
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga agcacctgac tacgaagtgc tatccgtgcg agaacagcta ttccacgaga
121ggatccgcga gtgtattata tcaacacttc tgtttgcaac actgtacatc ctctgccaca
181tcttcctgac ccgcttcaag aagcctgctg agttcaccac agtggatgat gaagatgcca
241ccgtcaacaa gattgcgctc gagctgtgca cctttaccct ggcaattgcc ctgggtgctg
301tcctgctcct gcccttctcc atcatcagca atgaggtgct gctctccctg cctcggaact
361actacatcca gtggctcaac ggctccctca tccatggcct ctggaacctt gtttttctct
421tctccaacct gtccctcatc ttcctcatgc cctttgcata tttcttcact gagtctgagg
481gctttgctgg ctccagaaag ggtgtcctgg gccgggtcta tgagacagtg gtgatgttga
541tgctcctcac tctgctggtg ctaggtatgg tgtgggtggc atcagccatt gtggacaaga
601acaaggccaa cagagagtca ctctatgact tttgggagta ctatctcccc tacctctact
661catgcatctc cttccttggg gttctgctgc tcctggtgtg tactccactg ggtctcgccc
721gcatgttctc cgtcactggg aagctgctag tcaagccccg gctgctggaa gacctggagg
781agcagctgta ctgctcagcc tttgaggagg cagccctgac ccgcaggatc tgtaatccta
841cttcctgctg gctgccttta gacatggagc tgctacacag acaggtcctg gctctgcaga
901cacagagggt cctgctggag aagaggcgga aggcttcagc ctggcaacgg aacctgggct
961accccctggc tatgctgtgc ttgctggtgc tgacgggcct gtctgttctc attgtggcca
1021tccacatcct ggagctgctc atcgatgagg ctgccatgcc ccgaggcatg cagggtacct
1081ccttaggcca ggtctccttc tccaagctgg gctcctttgg tgccgtcatt caggttgtac
1141tcatctttta cctaatggtg tcctcagttg tgggcttcta tagctctcca ctcttccgga
1201gcctgcggcc cagatggcac gacactgcca tgacgcagat aattgggaac tgtgtctgtc
1261tcctggtcct aagctcagca cttcctgtct tctctcgaac cctggggctc actcgctttg
1321acctgctggg tgactttgga cgcttcaact ggctgggcaa tttctacatt gtgttcctct
1381acaacgcagc ctttgcaggc ctcaccacac tctgtctggt gaagaccttc actgcagctg
1441tgcgggcaga gctgatccgg gcctttgggc tggacagact gccgctgccc gtctccggtt
1501tcccccaggc atctaggaag acccagcacc agtgcccaac tttcttgtac aaagtggttg
1561gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
1621accggtggcg cgttaagtcg acaatcaacc tctggatta

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