Transcript: Human XM_024452391.1

PREDICTED: Homo sapiens chromosome X open reading frame 40B (CXorf40B), transcript variant X17, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CXorf40B (541578)
Length:
2183
CDS:
626..1066

Additional Resources:

NCBI RefSeq record:
XM_024452391.1
NBCI Gene record:
CXorf40B (541578)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024452391.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000143181 GAAAGGAGGCAAGGATGTATT pLKO.1 1033 CDS 100% 13.200 6.600 Y CXorf40A n/a
2 TRCN0000163752 GAAAGGAGGCAAGGATGTATT pLKO.1 1033 CDS 100% 13.200 6.600 Y CXorf40B n/a
3 TRCN0000164541 CAGAAGTACCTGACTGTGATT pLKO.1 977 CDS 100% 4.950 2.475 Y CXorf40B n/a
4 TRCN0000140173 GACCAACCTGAAGCAGAAGTA pLKO.1 964 CDS 100% 4.950 2.475 Y CXorf40A n/a
5 TRCN0000165260 GACCAACCTGAAGCAGAAGTA pLKO.1 964 CDS 100% 4.950 2.475 Y CXorf40B n/a
6 TRCN0000163774 GATGAGGTTGTGGAACTAGAA pLKO.1 929 CDS 100% 4.950 2.475 Y CXorf40B n/a
7 TRCN0000122403 GCAGAAGTACCTGACTGTGAT pLKO.1 976 CDS 100% 4.950 2.475 Y CXorf40A n/a
8 TRCN0000140838 GCCTTATGCTGGCTTTGTCTT pLKO.1 655 CDS 100% 4.950 2.475 Y CXorf40A n/a
9 TRCN0000165839 GCCTTATGCTGGCTTTGTCTT pLKO.1 655 CDS 100% 4.950 2.475 Y CXorf40B n/a
10 TRCN0000159387 GCTTTGTCTTAAATGGAATCA pLKO.1 666 CDS 100% 4.950 2.475 Y CXorf40B n/a
11 TRCN0000139018 CGAAGACTTAACTCCCGATGA pLKO.1 913 CDS 100% 4.050 2.025 Y CXorf40A n/a
12 TRCN0000166567 CGAAGACTTAACTCCCGATGA pLKO.1 913 CDS 100% 4.050 2.025 Y CXorf40B n/a
13 TRCN0000139425 CATACCTAGGAAAGGAGGCAA pLKO.1 1024 CDS 100% 2.640 1.320 Y CXorf40A n/a
14 TRCN0000161094 GTACCTGACTGTGATTTCAAA pLKO.1 982 CDS 100% 0.000 0.000 Y CXorf40B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024452391.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05700 pDONR223 100% 91.9% 91.1% None 433_433delAinsGTAGAC;438_438delAins32 n/a
2 ccsbBroad304_05700 pLX_304 0% 91.9% 91.1% V5 433_433delAinsGTAGAC;438_438delAins32 n/a
3 TRCN0000478460 CTCCCGCACATACTTGTAGAACAG pLX_317 80.7% 91.9% 91.1% V5 433_433delAinsGTAGAC;438_438delAins32 n/a
4 ccsbBroadEn_04566 pDONR223 100% 91.3% 89.8% None (many diffs) n/a
5 ccsbBroad304_04566 pLX_304 0% 91.3% 89.8% V5 (many diffs) n/a
6 TRCN0000479481 AAGTGCCGTGGGACCCATAGTGGA pLX_317 56% 91.3% 89.8% V5 (many diffs) n/a
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