Transcript: Human XM_024452793.1

PREDICTED: Homo sapiens endoplasmic reticulum lectin 1 (ERLEC1), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ERLEC1 (27248)
Length:
4787
CDS:
195..1607

Additional Resources:

NCBI RefSeq record:
XM_024452793.1
NBCI Gene record:
ERLEC1 (27248)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024452793.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000168821 CGAGTACTACCTTGGGAATAT pLKO.1 635 CDS 100% 13.200 18.480 N ERLEC1 n/a
2 TRCN0000418863 AGCCTCACTCCTGTCAATATA pLKO_005 1384 CDS 100% 15.000 10.500 N ERLEC1 n/a
3 TRCN0000167332 CCTACAGAATTGAGTCTTATT pLKO.1 535 CDS 100% 13.200 9.240 N ERLEC1 n/a
4 TRCN0000167522 GCATATTGAATGGGCTAAGAA pLKO.1 1187 CDS 100% 5.625 3.938 N ERLEC1 n/a
5 TRCN0000166957 GAAGAATACTGCTAGAGCTTA pLKO.1 1205 CDS 100% 4.950 3.465 N ERLEC1 n/a
6 TRCN0000175150 GCTGAAGTTACAACTTGTGAA pLKO.1 873 CDS 100% 4.950 3.465 N Erlec1 n/a
7 TRCN0000168174 CAGATTCACCTCATGCTGTTA pLKO.1 1348 CDS 100% 4.950 2.970 N ERLEC1 n/a
8 TRCN0000074123 CGCCTGTAATCCCAACACTTT pLKO.1 3370 3UTR 100% 4.950 2.475 Y GJD4 n/a
9 TRCN0000166650 CGCCTGTAATCCCAACACTTT pLKO.1 3370 3UTR 100% 4.950 2.475 Y C9orf85 n/a
10 TRCN0000155229 GATCAAGACCATCCTGGCTAA pLKO.1 4756 3UTR 100% 4.050 2.025 Y INTS7 n/a
11 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2432 3UTR 100% 13.200 6.600 Y LIAS n/a
12 TRCN0000180546 GCCTGTAATCCCAGGACTTTA pLKO.1 4702 3UTR 100% 13.200 6.600 Y PRR11 n/a
13 TRCN0000008902 CCTCCCAAAGTGTTGGGATTA pLKO.1 1646 3UTR 100% 1.080 0.540 Y GPR83 n/a
14 TRCN0000156315 CCTCCCAAAGTGTTGGGATTA pLKO.1 1646 3UTR 100% 1.080 0.540 Y MYORG n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024452793.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03014 pDONR223 100% 89.7% 88% None (many diffs) n/a
2 ccsbBroad304_03014 pLX_304 0% 89.7% 88% V5 (many diffs) n/a
3 ccsbBroadEn_03013 pDONR223 100% 80.5% 79% None (many diffs) n/a
4 ccsbBroad304_03013 pLX_304 0% 80.5% 79% V5 (many diffs) n/a
5 TRCN0000481231 CATGTAGCCTGCTGTAGTCGTCAA pLX_317 31.3% 80.5% 79% V5 (many diffs) n/a
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