Transcript: Human XR_001738985.2

PREDICTED: Homo sapiens rhomboid domain containing 1 (RHBDD1), transcript variant X4, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RHBDD1 (84236)
Length:
4219
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001738985.2
NBCI Gene record:
RHBDD1 (84236)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001738985.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000075231 GCTCTGGATATCAGGATTATT pLKO.1 2519 3UTR 100% 15.000 21.000 N RHBDD1 n/a
2 TRCN0000075232 AGGACGGCAATACTACTTTAA pLKO.1 2487 3UTR 100% 13.200 18.480 N RHBDD1 n/a
3 TRCN0000430006 CGAACTTGTGGCTATTCATTT pLKO_005 2337 3UTR 100% 13.200 18.480 N RHBDD1 n/a
4 TRCN0000075229 GCCTATGTTATCACCGCATTT pLKO.1 2116 3UTR 100% 10.800 15.120 N RHBDD1 n/a
5 TRCN0000430750 TTTCCTGTACCGAACAGATTT pLKO_005 2305 3UTR 100% 13.200 10.560 N RHBDD1 n/a
6 TRCN0000414950 CCTTCACCATGCTGATGATTG pLKO_005 2019 3UTR 100% 10.800 7.560 N RHBDD1 n/a
7 TRCN0000435031 GTACTTACTGGAGTGGTATAC pLKO_005 2140 3UTR 100% 10.800 7.560 N RHBDD1 n/a
8 TRCN0000075230 GCTGGGATTCTTGTTGGACTA pLKO.1 2392 3UTR 100% 4.050 2.835 N RHBDD1 n/a
9 TRCN0000438489 GCGGCTTCACAGATTCGATAG pLKO_005 4201 3UTR 100% 6.000 8.400 N RHBDD1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001738985.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04344 pDONR223 100% 21.5% None (many diffs) n/a
2 ccsbBroad304_04344 pLX_304 0% 21.5% V5 (many diffs) n/a
3 TRCN0000468802 CATACGCTCTCGGCTAAGAACCAA pLX_317 15.9% 21.5% V5 (many diffs) n/a
4 ccsbBroadEn_12796 pDONR223 100% 6.5% None (many diffs) n/a
5 ccsbBroad304_12796 pLX_304 0% 6.5% V5 (many diffs) n/a
6 TRCN0000472049 GTTTGGATTGACCCCTTGCGAGGA pLX_317 100% 6.5% V5 (many diffs) n/a
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