Transcript: Human XR_002956974.1

PREDICTED: Homo sapiens cyclin and CBS domain divalent metal cation transport mediator 1 (CNNM1), transcript variant X2, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CNNM1 (26507)
Length:
6016
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002956974.1
NBCI Gene record:
CNNM1 (26507)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002956974.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000423938 ACGGTTCTGGAGGAGTTTAAG pLKO_005 1836 3UTR 100% 13.200 18.480 N CNNM1 n/a
2 TRCN0000437421 CCATTGTCCAGCGGGTGAATA pLKO_005 1876 3UTR 100% 13.200 18.480 N CNNM1 n/a
3 TRCN0000045224 CGGCATGACTTCTCCTTGTTT pLKO.1 2108 3UTR 100% 5.625 7.875 N CNNM1 n/a
4 TRCN0000425248 CCACTGCTTGCTCAGATAATG pLKO_005 2460 3UTR 100% 13.200 9.240 N CNNM1 n/a
5 TRCN0000045225 CCAGGAAGAAATGACTGACTT pLKO.1 2941 3UTR 100% 4.950 3.465 N CNNM1 n/a
6 TRCN0000045223 CGGCACAACATTGTGGACATT pLKO.1 1701 3UTR 100% 4.950 3.465 N CNNM1 n/a
7 TRCN0000045226 CTCCAATTTAACACCTCTGAT pLKO.1 3175 3UTR 100% 4.950 3.465 N CNNM1 n/a
8 TRCN0000045227 CCTGAAACATCCCAACGTGAT pLKO.1 2257 3UTR 100% 4.050 2.835 N CNNM1 n/a
9 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 4548 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002956974.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11827 pDONR223 100% 29.2% None (many diffs) n/a
2 ccsbBroad304_11827 pLX_304 0% 29.2% V5 (many diffs) n/a
3 ccsbBroadEn_15035 pDONR223 92.5% 29% None (many diffs) n/a
4 ccsbBroad304_15035 pLX_304 0% 29% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000476036 CTGATCTAGGTATCATCTTGCTCA pLX_317 19.4% 26.7% V5 (many diffs) n/a
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