Construct: ORF TRCN0000480692
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017449.1_s317c1
- Derived from:
- ccsbBroadEn_07263
- DNA Barcode:
- TTCAGCATCACCTTTAACGAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRPF18 (8559)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480692
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8559 | PRPF18 | pre-mRNA processing factor 18 | NM_003675.4 | 99.8% | 99.7% | 8T>C;549A>G |
| 2 | human | 8559 | PRPF18 | pre-mRNA processing factor 18 | XM_011519754.3 | 94.9% | 93.8% | (many diffs) |
| 3 | human | 8559 | PRPF18 | pre-mRNA processing factor 18 | XM_017016860.2 | 86% | 85.9% | 8T>C;549A>G;578_579ins141 |
| 4 | human | 8559 | PRPF18 | pre-mRNA processing factor 18 | XR_002957038.1 | 24.6% | (many diffs) | |
| 5 | human | 8559 | PRPF18 | pre-mRNA processing factor 18 | XR_001747251.2 | 10.5% | (many diffs) | |
| 6 | human | 8559 | PRPF18 | pre-mRNA processing factor 18 | XR_001747250.1 | 9.9% | (many diffs) | |
| 7 | human | 8559 | PRPF18 | pre-mRNA processing factor 18 | XR_002957037.1 | 7.5% | (many diffs) | |
| 8 | human | 8559 | PRPF18 | pre-mRNA processing factor 18 | XR_001747249.1 | 6.5% | (many diffs) | |
| 9 | mouse | 67229 | Prpf18 | pre-mRNA processing factor 18 | NM_026045.3 | 89.9% | 98.8% | (many diffs) |
| 10 | mouse | 67229 | Prpf18 | pre-mRNA processing factor 18 | XM_006497530.3 | 87.6% | 96.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1092
- ORF length:
- 1026
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cactctgaaa tcagagatcc ttcggaagcg gcagctggtg gaggacagga 121 acctgctggt ggaaaataaa aaatatttca agcgtagtga gctcgccaaa aaagaagagg 181 aagcatattt tgaaagatgt ggctacaaga tacagccaaa agaggaggac cagaaaccat 241 taacttcatc gaatccagtg ttagaacttg aactggcaga ggaaaaatta cctatgacgc 301 tttctaggca agaggtcatc agaagattga gagaaagagg agaaccaatc agactatttg 361 gagagactga ttatgatgct tttcaacgtt taaggaaaat agagatcctc acaccagaag 421 ttaacaaggg attgaggaat gatttgaaag cagccttgga taagattgat cagcagtacc 481 tcaatgaaat cgtcggcggt caggagcctg gagaggaaga cacacagaat gatctgaaag 541 ttcatgagga aaacaccaca attgaagagt tagaggcgct tggagagtcc ttagggaaag 601 gcgatgatca taaggacatg gacatcatca ccaaattcct gaagtttctt cttggcgttt 661 GGGCTAAAGA ATTGAATGCC AGAGAAGATT ATGTGAAACG CAGTGTGCAG GGTAAACTGA 721 ACAGTGCGAC CCAGAAACAG ACCGAGTCCT ACCTAAGACC ACTTTTTAGA AAGCTACGGA 781 AAAGGAATCT TCCTGCTGAT ATTAAAGAAT CAATAACGGA TATTATTAAA TTCATGTTGC 841 AGAGAGAATA CGTGAAGGCA AATGATGCTT ATCTTCAGAT GGCCATTGGA AATGCGCCTT 901 GGCCCATCGG TGTCACTATG GTTGGTATCC ATGCCAGAAC TGGCAGAGAA AAGATTTTTT 961 CCAAGCATGT TGCACATGTT TTAAATGACG AAACTCAGCG GAAATATATT CAGGGATTGA 1021 AGAGGTTAAT GACCATTTGC CAGAAACACT TTCCTACAGA CCCATCCAAA TGTGTGGAGT 1081 ACAATGCACT GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1141 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1201 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTC AGCATCACCT TTAACGAACC 1261 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t