Transcript: Mouse XR_379879.2

PREDICTED: Mus musculus F-box and leucine-rich repeat protein 2 (Fbxl2), transcript variant X2, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fbxl2 (72179)
Length:
1711
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_379879.2
NBCI Gene record:
Fbxl2 (72179)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_379879.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000092669 GCCGGAACATTGAACATTTAA pLKO.1 400 3UTR 100% 15.000 21.000 N Fbxl2 n/a
2 TRCN0000437561 ATCACTGACAGCACGTGTTAC pLKO_005 441 3UTR 100% 10.800 8.640 N Fbxl2 n/a
3 TRCN0000443607 TACACTGCTAGCTCGGAATTG pLKO_005 926 3UTR 100% 10.800 8.640 N Fbxl2 n/a
4 TRCN0000092672 CAGATCACAAAGGAAGGCATT pLKO.1 594 3UTR 100% 4.050 2.835 N Fbxl2 n/a
5 TRCN0000092670 CCTTGAAGAATGTGTCCTGAT pLKO.1 968 3UTR 100% 4.050 2.835 N Fbxl2 n/a
6 TRCN0000092671 CCTTAGCAGATTCTGTTCCAA pLKO.1 464 3UTR 100% 3.000 2.100 N Fbxl2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_379879.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02865 pDONR223 100% 65.2% None (many diffs) n/a
2 ccsbBroad304_02865 pLX_304 0% 65.2% V5 (many diffs) n/a
3 TRCN0000480745 CATTCGACCGAATATATATCTGAC pLX_317 31.4% 65.2% V5 (many diffs) n/a
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