Transcript: Mouse XR_389807.3

PREDICTED: Mus musculus predicted gene 7420 (Gm7420), misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Gm7420 (664949)
Length:
1172
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_389807.3
NBCI Gene record:
Gm7420 (664949)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_389807.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000234147 CAAAGTGTGTAAAGGACAAAC pLKO_005 176 3UTR 100% 10.800 7.560 N EG546908 n/a
2 TRCN0000239889 CAGAAAGCAGAGTACTCAAAT pLKO_005 254 3UTR 100% 13.200 6.600 Y Morf4l1b n/a
3 TRCN0000071525 CCTTGCTTTATTACTGAACTA pLKO.1 816 3UTR 100% 4.950 2.475 Y Morf4l1 n/a
4 TRCN0000288202 CCTTGCTTTATTACTGAACTA pLKO_005 816 3UTR 100% 4.950 2.475 Y Morf4l1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_389807.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02566 pDONR223 100% 56.8% None (many diffs) n/a
2 ccsbBroad304_02566 pLX_304 0% 56.8% V5 (many diffs) n/a
3 TRCN0000468389 GCTCCCCGGTCGGACCGAAGGCAC pLX_317 38.5% 56.8% V5 (many diffs) n/a
4 ccsbBroadEn_14059 pDONR223 100% 46.8% None (many diffs) n/a
5 ccsbBroad304_14059 pLX_304 0% 46.8% V5 (many diffs) n/a
6 ccsbBroadEn_15732 pDONR223 0% 46.8% None (many diffs) n/a
7 ccsbBroad304_15732 pLX_304 0% 46.8% V5 (many diffs) n/a
8 TRCN0000472961 ACCCGGTGTCATGCCACGCTAAAA pLX_317 76.3% 46.8% V5 (many diffs) n/a
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