Construct: ORF TRCN0000468389
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005264.1_s317c1
- Derived from:
- ccsbBroadEn_02566
- DNA Barcode:
- GCTCCCCGGTCGGACCGAAGGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MORF4L1 (10933)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468389
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10933 | MORF4L1 | mortality factor 4 like 1 | NM_006791.4 | 100% | 100% | |
| 2 | human | 10933 | MORF4L1 | mortality factor 4 like 1 | NM_206839.2 | 89.2% | 88.9% | 156_272del |
| 3 | human | 10933 | MORF4L1 | mortality factor 4 like 1 | NM_001265603.1 | 72.7% | 72.7% | 0_1ins264 |
| 4 | human | 10933 | MORF4L1 | mortality factor 4 like 1 | NM_001265604.1 | 72.7% | 72.7% | 0_1ins264 |
| 5 | human | 10933 | MORF4L1 | mortality factor 4 like 1 | NM_001265605.1 | 72.7% | 72.7% | 0_1ins264 |
| 6 | mouse | 627352 | Morf4l1b | mortality factor 4 like 1B | NM_001326287.1 | 90.8% | 99.6% | (many diffs) |
| 7 | mouse | 21761 | Morf4l1 | mortality factor 4 like 1 | NM_024431.3 | 90.7% | 100% | (many diffs) |
| 8 | mouse | 21761 | Morf4l1 | mortality factor 4 like 1 | XM_006511018.1 | 81.1% | 89.4% | (many diffs) |
| 9 | mouse | 21761 | Morf4l1 | mortality factor 4 like 1 | NM_001039147.2 | 80.9% | 88.9% | (many diffs) |
| 10 | mouse | 664949 | Gm7420 | predicted gene 7420 | XR_389807.3 | 56.8% | (many diffs) | |
| 11 | mouse | 624582 | Morf4l1-ps1 | mortality factor 4 like 1, ... | NR_038091.1 | 17% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1035
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gccgaagcag gacccgaagc ctaaattcca ggagggtgag cgagtgctgt 121 gctttcatgg gcctcttctt tatgaagcaa agtgtgtaaa ggttgccata aaggacaaac 181 aagtgaaata cttcatacat tacagtggtt ggaataaaaa ttgggatgaa tgggttccgg 241 agagcagagt actcaaatac gtggacacca atttgcagaa acagcgagaa cttcaaaaag 301 ccaatcagga gcagtatgca gaggggaaga tgagaggggc tgccccagga aagaagacat 361 ctggtctgca acagaaaaat gttgaagtga aaacgaaaaa gaacaaacag aaaacacctg 421 gaaatggaga tggtggcagt accagtgaga cccctcagcc tcctcggaag aaaagggccc 481 gggtagatcc tactgttgaa aatgaggaaa cattcatgaa cagagttgaa gttaaagtaa 541 agattcctga agagctaaaa ccgtggcttg ttgatgactg ggacttaatt accaggcaaa 601 aacagctctt ttatcttcct gccaagaaga atgtggattc cattcttgag gattatgcaa 661 attaCAAGAA ATCTCGTGGA AACACAGATA ATAAGGAGTA TGCGGTTAAT GAAGTTGTGG 721 CAGGGATAAA AGAATACTTC AACGTAATGT TGGGTACCCA GCTACTCTAT AAATTTGAGA 781 GACCACAGTA TGCTGAAATT CTTGCAGATC ATCCCGATGC ACCCATGTCC CAGGTGTATG 841 GAGCGCCACA TCTCCTGAGA TTATTTGTAC GAATTGGAGC AATGTTGGCT TATACACCTC 901 TGGATGAGAA GAGCCTTGCT TTATTACTCA ATTATCTTCA CGATTTCCTA AAGTACCTGG 961 CAAAGAATTC TGCAACTTTG TTCAGTGCCA GCGATTATGA AGTGGCTCCT CCTGAGTACC 1021 ATCGGAAAGC TGTGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCTCCCCGGT CGGACCGAAG 1201 GCACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt