Transcript: Mouse XR_876493.1

PREDICTED: Mus musculus serine/arginine-rich splicing factor 7 (Srsf7), transcript variant X3, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Srsf7 (225027)
Length:
2902
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_876493.1
NBCI Gene record:
Srsf7 (225027)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_876493.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000071933 CGTCGTCCCTTTGATCCTAAT pLKO.1 399 3UTR 100% 10.800 15.120 N Srsf7 n/a
2 TRCN0000324749 CGTCGTCCCTTTGATCCTAAT pLKO_005 399 3UTR 100% 10.800 15.120 N Srsf7 n/a
3 TRCN0000273401 GATCAAGATCCAGGTCTATTT pLKO_005 1307 3UTR 100% 13.200 10.560 N SRSF7 n/a
4 TRCN0000071935 GTTCGAGGATTGGATGGGAAA pLKO.1 300 3UTR 100% 4.050 3.240 N Srsf7 n/a
5 TRCN0000324747 GTTCGAGGATTGGATGGGAAA pLKO_005 300 3UTR 100% 4.050 3.240 N Srsf7 n/a
6 TRCN0000071937 CTGGTAAAGGAGAGTTAGAAA pLKO.1 175 3UTR 100% 5.625 3.938 N Srsf7 n/a
7 TRCN0000324683 CTGGTAAAGGAGAGTTAGAAA pLKO_005 175 3UTR 100% 5.625 3.938 N Srsf7 n/a
8 TRCN0000071936 CTGTGTCTCTTCGTAGATCAA pLKO.1 1244 3UTR 100% 4.950 3.465 N Srsf7 n/a
9 TRCN0000324748 CTGTGTCTCTTCGTAGATCAA pLKO_005 1244 3UTR 100% 4.950 3.465 N Srsf7 n/a
10 TRCN0000071934 GCTTATGACTGTCATCGCTAT pLKO.1 456 3UTR 100% 4.050 2.835 N Srsf7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_876493.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01525 pDONR223 100% 21.3% None (many diffs) n/a
2 ccsbBroad304_01525 pLX_304 0% 21.3% V5 (many diffs) n/a
3 TRCN0000467489 GCCCAGCAACTTAAATCGTCGGAT pLX_317 50.4% 21.3% V5 (many diffs) n/a
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