Construct: ORF TRCN0000467489

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015584.1_s317c1
Derived from:
ccsbBroadEn_01525
DNA Barcode:
GCCCAGCAACTTAAATCGTCGGAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SRSF7 (6432)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467489
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6432 SRSF7 serine and arginine rich sp... NM_001031684.3 100% 100%
2 human 6432 SRSF7 serine and arginine rich sp... NM_001363802.1 98.7% 98.7% 563_564insGTATTTCCA
3 human 6432 SRSF7 serine and arginine rich sp... NM_001195446.1 94.9% 94.9% 624_625ins36
4 human 6432 SRSF7 serine and arginine rich sp... XM_005264485.2 93.6% 93.6% 563_564insGTATTTCCA;615_616ins36
5 human 6432 SRSF7 serine and arginine rich sp... XR_001738892.1 57.9% (many diffs)
6 human 6432 SRSF7 serine and arginine rich sp... XM_011533032.2 49.5% 47.4% (many diffs)
7 human 6432 SRSF7 serine and arginine rich sp... XR_939711.1 44.8% (many diffs)
8 human 6432 SRSF7 serine and arginine rich sp... XR_001738894.1 44.2% (many diffs)
9 human 6432 SRSF7 serine and arginine rich sp... XR_939708.1 40.6% 1_105del;490_1182del;1513_1755del
10 human 6432 SRSF7 serine and arginine rich sp... XR_939709.1 40.1% (many diffs)
11 human 6432 SRSF7 serine and arginine rich sp... XR_939710.1 38.6% (many diffs)
12 human 6432 SRSF7 serine and arginine rich sp... XR_001738893.1 38.1% (many diffs)
13 mouse 225027 Srsf7 serine/arginine-rich splici... NM_146083.2 92.2% 98.3% (many diffs)
14 mouse 225027 Srsf7 serine/arginine-rich splici... NM_001195485.1 91% 97% (many diffs)
15 mouse 225027 Srsf7 serine/arginine-rich splici... NM_001195486.1 87.8% 94.1% (many diffs)
16 mouse 225027 Srsf7 serine/arginine-rich splici... XM_006524190.2 87.5% 93.2% (many diffs)
17 mouse 225027 Srsf7 serine/arginine-rich splici... NM_001195487.1 86.2% 92% (many diffs)
18 mouse 225027 Srsf7 serine/arginine-rich splici... XM_006524191.1 83% 89% (many diffs)
19 mouse 225027 Srsf7 serine/arginine-rich splici... XM_011246412.2 46.4% 3.7% (many diffs)
20 mouse 225027 Srsf7 serine/arginine-rich splici... XM_011246413.1 46.2% 45.7% (many diffs)
21 mouse 225027 Srsf7 serine/arginine-rich splici... XR_001782073.1 24.2% (many diffs)
22 mouse 225027 Srsf7 serine/arginine-rich splici... NR_036615.1 23.5% (many diffs)
23 mouse 225027 Srsf7 serine/arginine-rich splici... XR_876493.1 21.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
780
ORF length:
714
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc gcgttacggg cggtacggag gagaaaccaa ggtgtatgtt ggtaacctgg
121gaactggcgc tggcaaagga gagttagaaa gggctttcag ttattatggt cctttaagaa
181ctgtatggat tgcgagaaat cctccaggat ttgcctttgt ggaattcgaa gatcctagag
241atgcagaaga tgcagtacga ggactggatg gaaaggtgat ttgtggctcc cgagtgaggg
301ttgaactatc gacaggcatg cctcggagat cacgttttga tagaccacct gcccgacgtc
361cctttgatcc aaatgataga tgctatgagt gtggcgaaaa gggacattat gcttatgatt
421GTCATCGTTA CAGCCGGCGA AGAAGAAGCA GGTCACGGTC TAGATCACAT TCTCGATCCA
481GAGGAAGGCG ATACTCTCGC TCACGCAGCA GGAGCAGGGG ACGAAGGTCA AGGTCAGCAT
541CTCCTCGACG ATCAAGATCT ATCTCTCTTC GTAGATCAAG ATCAGCTTCA CTCAGAAGAT
601CTAGGTCTGG TTCTATAAAA GGATCGAGGT ATTTCCAATC CCCGTCGAGG TCAAGATCAA
661GATCCAGGTC TATTTCACGA CCAAGAAGCA GCCGATCAAA GTCCAGATCT CCATCTCCAA
721AAAGAAGTCG TTCCCCATCA GGAAGTCCTC GCAGAAGTGC AAGTCCTGAA AGAATGGACT
781GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
841GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
901TTTATATATC TTGTGGAAAG GACGAGCCCA GCAACTTAAA TCGTCGGATA CGCGTTAAGT
961Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)