Transcript: Human XR_937827.3

PREDICTED: Homo sapiens WBP2 N-terminal like (WBP2NL), transcript variant X1, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
WBP2NL (164684)
Length:
5640
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_937827.3
NBCI Gene record:
WBP2NL (164684)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_937827.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000139246 CCCGAGGATTTCCACTTAGAA pLKO.1 4546 3UTR 100% 5.625 7.875 N WBP2NL n/a
2 TRCN0000122766 CTCACTTCATACCGGGTGATT pLKO.1 4293 3UTR 100% 4.950 6.930 N WBP2NL n/a
3 TRCN0000144528 CAGATGCCTTGTTCAGTTATT pLKO.1 4635 3UTR 100% 13.200 9.240 N WBP2NL n/a
4 TRCN0000139558 CAGCGATCAGAAGGCTCAAAT pLKO.1 4239 3UTR 100% 13.200 9.240 N WBP2NL n/a
5 TRCN0000139933 GCGATCAGAAGGCTCAAATGT pLKO.1 4241 3UTR 100% 5.625 3.938 N WBP2NL n/a
6 TRCN0000139307 CTGATGACGAACCTCACTGTT pLKO.1 4371 3UTR 100% 4.950 3.465 N WBP2NL n/a
7 TRCN0000143390 GAATATGTGCACTCCACAGAT pLKO.1 4619 3UTR 100% 4.950 3.465 N WBP2NL n/a
8 TRCN0000139058 CTGGTTCAGCTCTATGGGAAT pLKO.1 4577 3UTR 100% 4.050 2.835 N WBP2NL n/a
9 TRCN0000139479 CCAAGCAAAGAGGTACCCTAA pLKO.1 5096 3UTR 100% 0.000 0.000 N WBP2NL n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_937827.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09759 pDONR223 100% 16.4% None (many diffs) n/a
2 ccsbBroad304_09759 pLX_304 0% 16.4% V5 (many diffs) n/a
3 TRCN0000465568 ACTTCCTGGAGAGGGGCTAACAGT pLX_317 28.7% 16.4% V5 (many diffs) n/a
4 TRCN0000467361 GTAATTTTTGTGACGAGATGCGTT pLX_317 71% 10.6% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_16117 pDONR223 0% 10.6% None (many diffs) n/a
6 ccsbBroad304_16117 pLX_304 0% 10.6% V5 (many diffs) n/a
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