Construct: ORF TRCN0000473164
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008998.1_s317c1
- Derived from:
- ccsbBroadEn_13072
- DNA Barcode:
- ACTATGTATCCCCCATGCCTCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZFYVE27 (118813)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473164
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_017015650.1 | 99.8% | 99.7% | 209G>T;951C>A |
| 2 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_011539254.2 | 98.3% | 98.2% | 209G>T;599_613del;966C>A |
| 3 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_011539255.2 | 98.3% | 98.2% | 209G>T;599_613del;966C>A |
| 4 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_017015651.2 | 94% | 93.8% | (many diffs) |
| 5 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_017015652.1 | 94% | 93.8% | (many diffs) |
| 6 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_005269508.4 | 92.7% | 92.5% | (many diffs) |
| 7 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_005269509.3 | 92.7% | 92.5% | (many diffs) |
| 8 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_017015653.2 | 92% | 91.8% | (many diffs) |
| 9 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_017015654.1 | 92% | 91.8% | (many diffs) |
| 10 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | NM_001174121.1 | 87.7% | 87.6% | (many diffs) |
| 11 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | NM_001174119.1 | 84.6% | 84.1% | (many diffs) |
| 12 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | NM_144588.7 | 83.2% | 83.2% | 1_204del;413G>T;1155C>A |
| 13 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_005269504.3 | 83.2% | 83.2% | 1_204del;413G>T;1155C>A |
| 14 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_017015648.1 | 82.8% | 76.1% | (many diffs) |
| 15 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_017015655.1 | 82.8% | 76.1% | (many diffs) |
| 16 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | NM_001002261.3 | 82.2% | 82.2% | (many diffs) |
| 17 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_005269502.3 | 82.2% | 82.2% | (many diffs) |
| 18 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_005269503.3 | 82.2% | 82.2% | (many diffs) |
| 19 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_005269510.3 | 81.7% | 75% | (many diffs) |
| 20 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_005269511.3 | 81.7% | 75% | (many diffs) |
| 21 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | NM_001002262.4 | 81.5% | 81.5% | (many diffs) |
| 22 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_005269506.3 | 81.5% | 81.5% | (many diffs) |
| 23 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | NM_001174120.1 | 80.9% | 74.1% | (many diffs) |
| 24 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_005269505.4 | 80.6% | 80.5% | (many diffs) |
| 25 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_017015647.1 | 80.6% | 80.5% | (many diffs) |
| 26 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_017015649.1 | 79.7% | 73.1% | (many diffs) |
| 27 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_011539252.2 | 77.2% | 77.4% | (many diffs) |
| 28 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_011539253.2 | 75.5% | 75.7% | (many diffs) |
| 29 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | NM_001174122.1 | 72.7% | 65.7% | (many diffs) |
| 30 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_017015644.1 | 67.7% | 67.5% | (many diffs) |
| 31 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_017015646.1 | 66.8% | 66.6% | (many diffs) |
| 32 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XM_017015645.1 | 66% | 65.8% | (many diffs) |
| 33 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XR_945594.2 | 57.2% | (many diffs) | |
| 34 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XR_945597.2 | 56.4% | (many diffs) | |
| 35 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XR_002956957.1 | 23.3% | (many diffs) | |
| 36 | human | 118813 | ZFYVE27 | zinc finger FYVE-type conta... | XR_002956956.1 | 22.5% | (many diffs) | |
| 37 | mouse | 319740 | Zfyve27 | zinc finger, FYVE domain co... | NM_177319.3 | 71% | 73.5% | (many diffs) |
| 38 | mouse | 319740 | Zfyve27 | zinc finger, FYVE domain co... | NM_001164531.1 | 69.4% | 71.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1095
- ORF length:
- 1029
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tttgtgttcc ttgctgacct gcctgggcct caacgtcttg ttcctcactt 121 tgaatgaggg tgcatggtac tcagtaggtg ccctgatgat ttcagtgccc gccctgctgg 181 gctaccttca ggaggtttgc cgggcacggc tgcctgattc cgagctgatg cggaggaagt 241 atcatagcgt gaggcaggag gacctgcaga gagttcgcct gtctcgtccc gaggccgtgg 301 ctgaggtgaa gagcttcttg atccagctgg aggccttcct gagccgcctg tgctgcacat 361 gtgaagccgc ctaccgcgtg ctgcactggg agaaccccgt cgtgtcctca cagttctatg 421 gggctcttct gggcacagtc tgcatgctgt atttgctgcc actctgctgg gttctcaccc 481 ttttaaacag cacgctcttt ctggggaatg tggagttctt ccgagttgtg tctgagtaca 541 gggcatctct gcagcagagg atgaacccaa agcaggaaga gcatgccttt gagagtcctc 601 caccaccaga tgttgggggg aaggatggtc tgatggacag cacgcctgcc ctcacaccca 661 cggaggaccT CACACCGGGC AGCGTGGAGG AGGCTGAGGA GGCTGAGCCA GATGAAGAGT 721 TTAAAGATGC GATTGAGGAG ACCCACTTGG TGGTGCTGGA GGATGATGAG GGCGCCCCGT 781 GCCCAGCAGA GGATGAGCTG GCCCTGCAGG ACAACGGGTT CCTGAGCAAG AATGAGGTGC 841 TGCGCAGCAA GGTGTCTCGG CTCACGGAGC GGCTCCGCAA GCGCTACCCC ACCAACAACT 901 TCGGGAACTG CACGGGCTGC TCGGCCACCT TCTCAGTGCT GAAGAAGAGG CGGAGCTGCA 961 GTAATTGTGG AAACAGCTTC TGCTCTCGAT GCTGCTCCTT CAAGGTGCCC AAGTCATCCA 1021 TGGGGGCCAC AGCCCCTGAA GCCCAGAGGG AGACTGTGTT TGTGTGTGCC TCGTGTAACC 1081 AGACCTTGAG CAAGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1141 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1201 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACTATGTATC CCCCATGCCT 1261 CGCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt