Construct: ORF TRCN0000473640

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002714.1_s317c1
Derived from:
ccsbBroadEn_14237
DNA Barcode:
AAGAATCTGTTACTACTTCAAATT
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PTBP2 (58155)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473640
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 58155 PTBP2 polypyrimidine tract bindin... NM_001300989.2 99.9% 100% 1584C>A
2 human 58155 PTBP2 polypyrimidine tract bindin... NM_021190.3 99.7% 99.8% 1466_1467insAAG;1581C>A
3 human 58155 PTBP2 polypyrimidine tract bindin... NM_001300985.2 99% 99% 903_917del;1599C>A
4 human 58155 PTBP2 polypyrimidine tract bindin... NM_001300988.1 98.8% 98.8% 903_917del;1481_1482insAAG;1596C>A
5 human 58155 PTBP2 polypyrimidine tract bindin... NM_001300986.2 98.4% 98.5% 8_31del;1608C>A
6 human 58155 PTBP2 polypyrimidine tract bindin... XM_011541875.2 98.2% 98.3% 8_31del;1490_1491insAAG;1605C>A
7 human 58155 PTBP2 polypyrimidine tract bindin... XM_011541876.2 97.5% 97.6% 8_31del;927_941del;1623C>A
8 human 58155 PTBP2 polypyrimidine tract bindin... XM_011541874.2 97.3% 97.4% (many diffs)
9 human 58155 PTBP2 polypyrimidine tract bindin... NM_001300987.2 95.8% 95.2% (many diffs)
10 human 58155 PTBP2 polypyrimidine tract bindin... XM_017001951.1 95.8% 95.2% (many diffs)
11 human 58155 PTBP2 polypyrimidine tract bindin... NM_001300990.2 90.1% 90.2% 0_1ins156;1428C>A
12 human 58155 PTBP2 polypyrimidine tract bindin... XR_001737347.2 49.9% (many diffs)
13 human 58155 PTBP2 polypyrimidine tract bindin... XR_001737346.2 49.7% (many diffs)
14 human 58155 PTBP2 polypyrimidine tract bindin... XR_946723.3 49.2% (many diffs)
15 human 58155 PTBP2 polypyrimidine tract bindin... XR_946722.2 49.1% (many diffs)
16 human 58155 PTBP2 polypyrimidine tract bindin... XR_946721.2 49% (many diffs)
17 human 58155 PTBP2 polypyrimidine tract bindin... XR_946720.2 48.9% (many diffs)
18 human 58155 PTBP2 polypyrimidine tract bindin... NR_125356.1 45.6% (many diffs)
19 human 58155 PTBP2 polypyrimidine tract bindin... NR_125357.2 12.9% (many diffs)
20 mouse 56195 Ptbp2 polypyrimidine tract bindin... NM_019550.2 93.6% 99.6% (many diffs)
21 mouse 56195 Ptbp2 polypyrimidine tract bindin... NM_001310711.1 93.4% 99.4% (many diffs)
22 mouse 56195 Ptbp2 polypyrimidine tract bindin... XM_006501731.1 92.9% 98.6% (many diffs)
23 mouse 56195 Ptbp2 polypyrimidine tract bindin... XM_006501732.1 92.7% 98.5% (many diffs)
24 mouse 56195 Ptbp2 polypyrimidine tract bindin... XR_001783719.1 41.3% (many diffs)
25 mouse 56195 Ptbp2 polypyrimidine tract bindin... XR_375550.2 40.4% (many diffs)
26 mouse 56195 Ptbp2 polypyrimidine tract bindin... XR_001783718.1 38.5% (many diffs)
27 mouse 56195 Ptbp2 polypyrimidine tract bindin... XR_375549.1 38.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1662
ORF length:
1596
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga cggaatcgtc actgaggttg cagttggcgt gaagagagga tctgacgaac
121tactctcagg cagtgttctc agtagtccga actctaatat gagcagcatg gtagttacag
181ccaatggtaa tgatagtaaa aaatttaaag gagaagataa aatggatggt gctccttctc
241gtgtacttca tattcgaaaa ttacctgggg aagtaacaga aactgaagtt attgctttag
301gcttaccttt tggtaaggtg accaacatcc ttatgctgaa aggaaaaaat caggcatttt
361tggaactagc aaccgaggaa gcagctatta ctatggttaa ttactattct gctgtgacac
421ctcatcttcg taaccaacca atatatatcc agtactcgaa tcacaaagaa ctaaagacag
481ataatacatt aaaccaacgt gctcaggcag ttcttcaagc tgtgacagct gtccagacag
541caaatactcc tcttagtggc accacagtta gcgagagtgc agtgactcca gcccagagtc
601cagtacttag aataattatt gacaacatgt actaccctgt aacacttgat gttcttcacc
661aaatattttc taagtttggt gctgtattga agataatcac atttacaaaa aataaccagt
721ttcaagcttt gctccagtat ggtgatccag taaatgctca acaagcaaaa ctagccctag
781atggtcagaa tatttataat gcctgctgta ccctaaggat tgatttttcc aaacttgtga
841atttgaatgt aaaatacaac aatgataaaa gtagggatta tactcgacct gatcttccat
901ctggggatgg acaacctgca ttggacccag ctattgctgc agcatttgcc aaggagacat
961ccctcttagc tgttccagga gctctgagtc ctttggccat tccaaatgct gctgcagcag
1021ctgctgcagc tgctgctggc cgagtgggta tgcctggagt ctcagctggt ggcaatacag
1081tcctgttggt tagcaattta aatgaagaga tggttacgcc ccaaagtctg tttaccctcT
1141TCGGTGTTTA TGGAGATGTG CAGCGTGTGA AGATTTTATA CAATAAGAAA GACAGCGCTC
1201TAATACAGAT GGCTGATGGA AACCAATCAC AACTTGCCAT GAATCATCTT AATGGACAGA
1261AAATGTATGG AAAAATTATT CGTGTTACTC TGTCTAAACA TCAGACTGTA CAGCTACCTC
1321GAGAGGGACT TGATGATCAA GGGCTAACAA AAGATTTTGG TAATTCCCCA TTGCATCGTT
1381TTAAGAAACC TGGATCCAAA AATTTTCAAA ACATTTTTCC TCCTTCTGCC ACCCTTCACC
1441TATCTAATAT CCCTCCATCA GTAGCAGAAG AGGATCTACG AACACTGTTC GCTAACACTG
1501GGGGCACTGT GAAAGCATTT AAGTTTTTTC AAAGAGATCA CAAAATGGCT CTTCTTCAGA
1561TGGCAACAGT GGAAGAAGCT ATTCAGGCCT TGATTGATCT TCATAATTAT AACCTTGGAG
1621AAAACCATCA TCTGAGAGTG TCTTTCTCAA AGTCAACAAT TTACCAACTT TCTTGTACAA
1681AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA
1741ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA
1801GGACGAAAGA ATCTGTTACT ACTTCAAATT ACGCGTTAAG TCgacaatca acctctggat
1861tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)