Transcript: Human XR_946794.3

PREDICTED: Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant X1, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MAP3K6 (9064)
Length:
4897
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_946794.3
NBCI Gene record:
MAP3K6 (9064)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_946794.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000002345 CGTGGAGAAGATGCAGTATTA pLKO.1 2724 3UTR 100% 13.200 9.240 N MAP3K6 n/a
2 TRCN0000352611 CGTGGAGAAGATGCAGTATTA pLKO_005 2724 3UTR 100% 13.200 9.240 N MAP3K6 n/a
3 TRCN0000197175 GCTTCAGCATGACCAACAATG pLKO.1 1844 3UTR 100% 10.800 7.560 N MAP3K6 n/a
4 TRCN0000002343 GTTGGAGTTTGATTATGAGTA pLKO.1 3345 3UTR 100% 4.950 3.465 N MAP3K6 n/a
5 TRCN0000352677 GTTGGAGTTTGATTATGAGTA pLKO_005 3345 3UTR 100% 4.950 3.465 N MAP3K6 n/a
6 TRCN0000199055 CAAGCCTTTCTCCTCCGAACT pLKO.1 4069 3UTR 100% 4.050 2.835 N MAP3K6 n/a
7 TRCN0000352612 CAAGCCTTTCTCCTCCGAACT pLKO_005 4069 3UTR 100% 4.050 2.835 N MAP3K6 n/a
8 TRCN0000002344 CATGTTCTTCAGCTCGGGTTT pLKO.1 2517 3UTR 100% 4.050 2.835 N MAP3K6 n/a
9 TRCN0000002342 CAACCATTCAAGACAGCCTGT pLKO.1 2968 3UTR 100% 2.160 1.512 N MAP3K6 n/a
10 TRCN0000126745 GCCTGCATATCGAACCTTTAT pLKO.1 822 3UTR 100% 13.200 6.600 Y Utp15 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_946794.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11332 pDONR223 100% 48.4% None (many diffs) n/a
2 ccsbBroad304_11332 pLX_304 0% 48.4% V5 (many diffs) n/a
3 TRCN0000481050 TGATCAGTTTTTGCCATGAGCGTT pLX_317 11.9% 48.4% V5 (many diffs) n/a
4 ccsbBroadEn_14926 pDONR223 0% 48.4% None (many diffs) n/a
5 ccsbBroad304_14926 pLX_304 0% 48.4% V5 (many diffs) n/a
6 TRCN0000465376 CGCAATTACCGCCGTCAACACTGT pLX_317 11.6% 48.4% V5 (many diffs) n/a
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