Construct: sgRNA BRDN0001146019

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTCCCACAAAATGTGGTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGK3 (23678)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75540

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 66841088 - SGK3 NNNCCCACAAAATGTGGTAG NGG 0 1.0 Tier I
2 NC_000008.11 8 66841088 - C8orf44-SGK3 NNNCCCACAAAATGTGGTAG NGG 0 1.0 Tier I
3 NC_000011.10 11 27442261 - LGR4 NNNCCCACAAAATGTGGTAT NGG 1 0.7 Tier II
4 NC_000013.11 13 69887912 - KLHL1 NNNCCCAAAAAATGTGGAAG NGG 2 0.4333 Tier II
5 NC_000015.10 15 27916946 + OCA2 NNNCCCTCAAAATGTGGAAG NGG 2 0.2917 Tier II
6 NC_000018.10 18 80032927 - TXNL4A NNNCAGACAAAATGTGGTAG NGG 2 0.2857 Tier II
7 NC_000002.12 2 86776463 - RMND5A NNNCCCACAAAATGTGGTGT NGG 2 0.2625 Tier II
8 NC_000001.11 1 26167139 - C1orf232 NNNCACACAAAATGTGGCAG NGG 2 0.2449 Tier II
9 NC_000001.11 1 232510018 - SIPA1L2 NNNCCCACAAAATGAGGGAG NGG 2 0.193 Tier II
10 NC_000016.10 16 20552984 - ACSM2B NNNCCCAAAAAATTTGGTAG NGG 2 0.1733 Tier II
11 NC_000010.11 10 52316032 - DKK1 NNNCCCACCAACTGTGGTAG NGG 2 0.1504 Tier II
12 NC_000023.11 X 53650173 + HUWE1 NNNCCCACTAAATGTGTTAG NGG 2 0.15 Tier II
13 NC_000016.10 16 28593850 - SULT1A2 NNNCCCACAAAATTTGGTGG NGG 2 0.1 Tier II
14 NC_000016.10 16 28607359 - SULT1A1 NNNCCCACAAAATTTGGTGG NGG 2 0.1 Tier II
15 NC_000012.12 12 23662543 - SOX5 NNNCCCACAAAATGTGGTTG NTG 2 0.021 Tier II
16 NC_000020.11 20 3625427 - ATRN NNNCCCAAAAAATGTGGTAG NGT 2 0.0105 Tier II
17 NC_000020.11 20 34744565 - NCOA6 NNNCCCACAAAATTTGGTAG NTG 2 0.0104 Tier II
18 NC_000004.12 4 161688484 - FSTL5 NNNCCCACAAAATGTGGTAG NAA 2 0.0 Tier II
19 NC_000009.12 9 40514578 + LOC102724580 NNNCCAACTAAATGTGGTAG NGG 2 0.5571 Tier III
20 NC_000009.12 9 64654552 + LOC101929583 NNNCCAACTAAATGTGGTAG NGG 2 0.5571 Tier III
21 NC_000009.12 9 65159747 + LOC105379443 NNNCCAACTAAATGTGGTAG NGG 2 0.5571 Tier III
22 NC_000009.12 9 65861797 - LOC101928195 NNNCCAACTAAATGTGGTAG NGG 2 0.5571 Tier III
23 NC_000020.11 20 24322452 + LOC105372577 NNNCACACAAAATGTGATAG NGG 2 0.5333 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 37728104 - Pcdhga8 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier I
2 NC_000069.6 3 104076404 + Magi3 NNNCCTACAAAATGTGGTAG NGG 1 0.9286 Tier II
3 NC_000077.6 11 30999978 - Asb3 NNNTCCAAAAAATGTGGTAG NGG 2 0.52 Tier II
4 NC_000071.6 5 48584105 - Kcnip4 NNNGCCATAAAATGTGGTAG NGG 2 0.4375 Tier II
5 NC_000082.6 16 38929337 + Igsf11 NNNCCCACAAAACGTGGTTG NGG 2 0.4251 Tier II
6 NC_000067.6 1 60005045 + Ica1l NNNCACACAAAATGTGGTAT NGG 2 0.4 Tier II
7 NC_000068.7 2 160893465 + Lpin3 NNNCCCAAAAAATGAGGTAG NGG 2 0.3763 Tier II
8 NC_000084.6 18 37728104 - Pcdhgb1 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier II
9 NC_000084.6 18 37728104 - Pcdhgb2 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier II
10 NC_000084.6 18 37728104 - Pcdhgb4 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier II
11 NC_000084.6 18 37728104 - Pcdhga1 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier II
12 NC_000084.6 18 37728104 - Pcdhga2 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier II
13 NC_000084.6 18 37728104 - Pcdhga3 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier II
14 NC_000084.6 18 37728104 - Pcdhga4 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier II
15 NC_000084.6 18 37728104 - Pcdhga5 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier II
16 NC_000084.6 18 37728104 - Pcdhga6 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier II
17 NC_000084.6 18 37728104 - Pcdhga7 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier II
18 NC_000084.6 18 37728104 - Gm37013 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier II
19 NC_000084.6 18 37728104 - Gm38666 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier II
20 NC_000084.6 18 37728104 - Gm38667 NNNCCCACAAAATGTGATGG NGG 2 0.35 Tier II
21 NC_000075.6 9 32446767 - Fli1 NNNCCCATAAAATGTGGGAG NGG 2 0.2917 Tier II
22 NC_000083.6 17 68582791 + L3mbtl4 NNNCCTACAAAATGTGGTAG NAG 2 0.2407 Tier II
23 NC_000069.6 3 123364738 - Sec24d NNNCCCACCAAATGTGGTAG NAG 2 0.1481 Tier II
24 NC_000070.6 4 133729009 + Arid1a NNNCCCACACCATGTGGTAG NGG 2 0.1333 Tier II
25 NC_000082.6 16 38929337 + Gm34258 NNNCCCACAAAACGTGGTTG NGG 2 0.4251 Tier III
26 NC_000080.6 14 54087329 - Tcra NNNCCCACAACATGTGGTAG NTG 2 0.0156 Tier III
27 NC_000080.6 14 54087329 - Tcrd NNNCCCACAACATGTGGTAG NTG 2 0.0156 Tier III
28 NC_000080.6 14 54087329 - Gm30275 NNNCCCACAACATGTGGTAG NTG 2 0.0156 Tier III
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Other clones with same target sequence:

(none)