Construct: sgRNA BRDN0001147975
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGCCGCAGAAACATAAATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDK1 (5163)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77009
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
172558738 |
- |
PDK1 |
NNNCCGCAGAAACATAAATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
49612558 |
+ |
RHAG |
NNNCGGCAGAAACATAAATG |
NGG |
1 |
0.6 |
Tier II |
| 3 |
NC_000008.11 |
8 |
14944315 |
+ |
SGCZ |
NNNCAACAGAAACATAAATG |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000006.12 |
6 |
26368295 |
- |
BTN3A2 |
NNNCTTCAGAAACATAAATG |
NGG |
2 |
0.4242 |
Tier II |
| 5 |
NC_000006.12 |
6 |
26443687 |
- |
BTN3A3 |
NNNCTTCAGAAACATAAATG |
NGG |
2 |
0.4242 |
Tier II |
| 6 |
NC_000001.11 |
1 |
167002999 |
- |
MAEL |
NNNCCGCAGAGACAAAAATG |
NGG |
2 |
0.3763 |
Tier II |
| 7 |
NC_000015.10 |
15 |
49352069 |
- |
GALK2 |
NNNCCCCAGACACATAAATG |
NGG |
2 |
0.2727 |
Tier II |
| 8 |
NC_000015.10 |
15 |
49352069 |
- |
FAM227B |
NNNCCCCAGACACATAAATG |
NGG |
2 |
0.2727 |
Tier II |
| 9 |
NC_000004.12 |
4 |
152846576 |
- |
ARFIP1 |
NNNCCTCAGAAAAATAAATG |
NGG |
2 |
0.2564 |
Tier II |
| 10 |
NC_000018.10 |
18 |
45504778 |
+ |
SLC14A2 |
NNNCTGCAGAAACACAAATG |
NGG |
2 |
0.1736 |
Tier II |
| 11 |
NC_000002.12 |
2 |
159151302 |
- |
TANC1 |
NNNCTGCAGAAACATAAATG |
NAG |
2 |
0.165 |
Tier II |
| 12 |
NC_000014.9 |
14 |
103089683 |
- |
LBHD2 |
NNNCTGCAGAAACATAAAGG |
NGG |
2 |
0.1591 |
Tier II |
| 13 |
NC_000005.10 |
5 |
78520172 |
- |
LHFPL2 |
NNNCGGCAGAAACATAAATG |
NTG |
2 |
0.0234 |
Tier II |
| 14 |
NC_000006.12 |
6 |
109476423 |
+ |
ZBTB24 |
NNNCCACAGAAACATAAATG |
NGC |
2 |
0.0222 |
Tier II |
| 15 |
NC_000012.12 |
12 |
107323569 |
- |
ABTB3 |
NNNCCACAGAAACATCAATG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000004.12 |
4 |
15260813 |
- |
C1QTNF7-AS1 |
NNNGAGCAGAAACATAAATG |
NGG |
2 |
0.2857 |
Tier III |
| 17 |
NC_000018.10 |
18 |
45504778 |
+ |
SLC14A2-AS1 |
NNNCTGCAGAAACACAAATG |
NGG |
2 |
0.1736 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
71875436 |
- |
Pdk1 |
NNNTCGCAGAAACATAAACG |
NGG |
2 |
0.2207 |
Tier I |
| 2 |
NC_000082.6 |
16 |
48455811 |
- |
Morc1 |
NNNACGCAGAAACATAAATA |
NGG |
2 |
0.7895 |
Tier II |
| 3 |
NC_000075.6 |
9 |
96265408 |
+ |
Tfdp2 |
NNNCTACAGAAACATAAATG |
NGG |
2 |
0.6364 |
Tier II |
| 4 |
NC_000069.6 |
3 |
55133288 |
+ |
Spg20 |
NNNTTGCAGAAACATAAATG |
NGG |
2 |
0.5091 |
Tier II |
| 5 |
NC_000084.6 |
18 |
64631789 |
+ |
Atp8b1 |
NNNCCCCAGAAACATAAGTG |
NGG |
2 |
0.2727 |
Tier II |
| 6 |
NC_000068.7 |
2 |
41107864 |
+ |
Lrp1b |
NNNCAGGAGAAACATAAATG |
NGG |
2 |
0.2689 |
Tier II |
| 7 |
NC_000085.6 |
19 |
42248761 |
- |
Golga7b |
NNNCCCCAGAAACATGAATG |
NGG |
2 |
0.1311 |
Tier II |
| 8 |
NC_000084.6 |
18 |
37739973 |
- |
Pcdhgb1 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 9 |
NC_000084.6 |
18 |
37739973 |
- |
Pcdhgb2 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 10 |
NC_000084.6 |
18 |
37739973 |
- |
Pcdhgb4 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 11 |
NC_000084.6 |
18 |
37739973 |
- |
Pcdhgb5 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 12 |
NC_000084.6 |
18 |
37739973 |
- |
Pcdhga1 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 13 |
NC_000084.6 |
18 |
37739973 |
- |
Pcdhga2 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 14 |
NC_000084.6 |
18 |
37739973 |
- |
Pcdhga3 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 15 |
NC_000084.6 |
18 |
37739973 |
- |
Pcdhga4 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 16 |
NC_000084.6 |
18 |
37739973 |
- |
Pcdhga5 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 17 |
NC_000084.6 |
18 |
37739973 |
- |
Pcdhga6 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 18 |
NC_000084.6 |
18 |
37739973 |
- |
Pcdhga7 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 19 |
NC_000084.6 |
18 |
37739973 |
- |
Pcdhga8 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 20 |
NC_000084.6 |
18 |
37739973 |
- |
Pcdhga9 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 21 |
NC_000084.6 |
18 |
37739973 |
- |
Gm37013 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 22 |
NC_000084.6 |
18 |
37739973 |
- |
Gm38666 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 23 |
NC_000084.6 |
18 |
37739973 |
- |
Gm38667 |
NNNTCGCAGAAACAGAAATG |
NGG |
2 |
0.04 |
Tier II |
| 24 |
NC_000067.6 |
1 |
89770006 |
- |
Agap1 |
NNNCCACAGAAACATAAATG |
NTG |
2 |
0.039 |
Tier II |
Other clones with same target sequence:
(none)
