Construct: sgRNA BRDN0001148423
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGCCAGGGATCTTACTAT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- MPP2 (4355)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NC_000017.11 | 17 | 43898280 | + | MPP2 | NNNGCCAGGGATCTTACTAT | NGG | 0 | 1.0 | Tier I |
| 2 | NC_000001.11 | 1 | 7097522 | - | CAMTA1 | NNNGCCAGGGGTCTTACAAT | NGG | 2 | 0.4333 | Tier II |
| 3 | NC_000010.11 | 10 | 27036125 | - | ANKRD26 | NNNGCCAAGGCTCTTACTAT | NGG | 2 | 0.4 | Tier II |
| 4 | NC_000023.11 | X | 81226267 | - | SH3BGRL | NNNGCCAGGGATCTTACCTT | NGG | 2 | 0.2308 | Tier II |
| 5 | NC_000008.11 | 8 | 120637640 | + | SNTB1 | NNNGCCAGGCTTCTTACTAT | NGG | 2 | 0.1231 | Tier II |
| 6 | NC_000005.10 | 5 | 141452604 | - | PCDHGB4 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 7 | NC_000005.10 | 5 | 141452604 | - | PCDHGA8 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 8 | NC_000005.10 | 5 | 141452604 | - | PCDHGA12 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 9 | NC_000005.10 | 5 | 141452604 | - | PCDHGB7 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 10 | NC_000005.10 | 5 | 141452604 | - | PCDHGB6 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 11 | NC_000005.10 | 5 | 141452604 | - | PCDHGB5 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 12 | NC_000005.10 | 5 | 141452604 | - | PCDHGB3 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 13 | NC_000005.10 | 5 | 141452604 | - | PCDHGB2 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 14 | NC_000005.10 | 5 | 141452604 | - | PCDHGB1 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 15 | NC_000005.10 | 5 | 141452604 | - | PCDHGA11 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 16 | NC_000005.10 | 5 | 141452604 | - | PCDHGA10 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 17 | NC_000005.10 | 5 | 141452604 | - | PCDHGA9 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 18 | NC_000005.10 | 5 | 141452604 | - | PCDHGA7 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 19 | NC_000005.10 | 5 | 141452604 | - | PCDHGA6 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 20 | NC_000005.10 | 5 | 141452604 | - | PCDHGA5 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 21 | NC_000005.10 | 5 | 141452604 | - | PCDHGA4 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 22 | NC_000005.10 | 5 | 141452604 | - | PCDHGA3 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 23 | NC_000005.10 | 5 | 141452604 | - | PCDHGA2 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 24 | NC_000005.10 | 5 | 141452604 | - | PCDHGA1 | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier II |
| 25 | NC_000006.12 | 6 | 57296421 | + | PRIM2 | NNNGCCAGGGATCTCACTGT | NGG | 2 | 0.1023 | Tier II |
| 26 | NC_000015.10 | 15 | 68560812 | + | CORO2B | NNNGCCAGTGATCTTACTAG | NGG | 2 | 0.0941 | Tier II |
| 27 | NC_000005.10 | 5 | 177365084 | - | RGS14 | NNNGCCAGTGATCTTACTAT | NGC | 2 | 0.0119 | Tier II |
| 28 | NC_000011.10 | 11 | 17925758 | - | SERGEF | NNNGCCAGGGATCTTGCTAT | NGT | 2 | 0.0031 | Tier II |
| 29 | NC_000003.12 | 3 | 108726689 | + | LOC105374033 | NNNGCCAGGTCTCTTACTAT | NGG | 2 | 0.325 | Tier III |
| 30 | NC_000008.11 | 8 | 120637640 | + | NCAPGP1 | NNNGCCAGGCTTCTTACTAT | NGG | 2 | 0.1231 | Tier III |
| 31 | NC_000005.10 | 5 | 141452604 | - | PCDHG@ | NNNGCCAGGGTTCTTACTGT | NGG | 2 | 0.1154 | Tier III |
| 32 | NC_000004.12 | 4 | 148936873 | - | LOC107986195 | NNNGCCAGGGATCTTTCTTT | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NC_000077.6 | 11 | 102080671 | + | Mpp2 | NNNGCCAGGGATCTGACTAT | NGG | 1 | 0.05 | Tier I |
| 2 | NC_000075.6 | 9 | 64330248 | + | Dis3l | NNNGCCTGGGATCTTACTTT | NGG | 2 | 0.2356 | Tier II |
| 3 | NC_000075.6 | 9 | 100482305 | - | Il20rb | NNNGCCAGGGGTCTTACTAT | NAG | 2 | 0.1685 | Tier II |
| 4 | NC_000070.6 | 4 | 24628025 | + | Klhl32 | NNNGCCAGGGATCCTACCAT | NGG | 2 | 0.1224 | Tier II |
| 5 | NC_000068.7 | 2 | 152765274 | - | Bcl2l1 | NNNGCCTGGGATCTTACTAT | NAG | 2 | 0.1134 | Tier II |
| 6 | NC_000068.7 | 2 | 156991208 | - | Ndrg3 | NNNGCCAGGGATCTTGCTAA | NGG | 2 | 0.1082 | Tier II |
| 7 | NC_000082.6 | 16 | 96686068 | + | Dscam | NNNGCCAGGGATCTCACTAT | NAG | 2 | 0.0707 | Tier II |
| 8 | NC_000073.6 | 7 | 143193368 | + | Kcnq1 | NNNGCCAGGGATCTTACTGG | NGG | 2 | 0.0662 | Tier II |
| 9 | NC_000080.6 | 14 | 66148407 | - | Chrna2 | NNNGACAGGGATCTTACTAT | NTG | 2 | 0.0223 | Tier II |
| 10 | NC_000071.6 | 5 | 34696832 | + | Grk4 | NNNGCCAGGGATCTGACTAC | NGG | 2 | 0.0045 | Tier II |
| 11 | NC_000068.7 | 2 | 179628385 | + | Cdh4 | NNNGCCAGGGCTCTTCCTAT | NGG | 2 | 0.0 | Tier II |