Construct: sgRNA BRDN0001149342

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAGTTCTAACCAATAAGAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NME5 (8382)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76570

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 138129327 + NME5 NNNTTCTAACCAATAAGAGA NGG 0 1.0 Tier I
2 NC_000013.11 13 98989916 + DOCK9 NNNTTCAAACCAATAAGAAA NGG 2 0.625 Tier II
3 NC_000001.11 1 163283607 + RGS5 NNNTTCTAAACAATAAGATA NGG 2 0.5778 Tier II
4 NC_000003.12 3 85384621 - CADM2 NNNTTCTGACCAATAAGAAA NGG 2 0.5238 Tier II
5 NC_000017.11 17 59194505 - PRR11 NNNTGCTAACAAATAAGAGA NGG 2 0.48 Tier II
6 NC_000002.12 2 218133835 - CXCR2 NNNTGCTACCCAATAAGAGA NGG 2 0.3657 Tier II
7 NC_000013.11 13 91783921 + GPC5 NNNTTCTTACCAATGAGAGA NGG 2 0.2786 Tier II
8 NC_000002.12 2 143995365 + GTDC1 NNNTTTTAACCAATAAGAGA NAG 2 0.2407 Tier II
9 NC_000003.12 3 3848694 - LRRN1 NNNTTCTCACCAATAAGTGA NGG 2 0.2143 Tier II
10 NC_000023.11 X 139544881 - F9 NNNTTCTAACCAATGAGAGA NAG 2 0.1685 Tier II
11 NC_000008.11 8 118555120 - SAMD12 NNNTGCTAACCAATAACAGA NGG 2 0.1506 Tier II
12 NC_000001.11 1 67347729 + IL12RB2 NNNTTCTTACCAATAGGAGA NGG 2 0.0824 Tier II
13 NC_000023.11 X 11635194 - ARHGAP6 NNNTTATAACCAATAAGAGA NGA 2 0.0645 Tier II
14 NC_000005.10 5 141463758 - PCDHGB4 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
15 NC_000005.10 5 141463758 - PCDHGA8 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
16 NC_000005.10 5 141463758 - PCDHGA12 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
17 NC_000005.10 5 141463758 - PCDHGB7 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
18 NC_000005.10 5 141463758 - PCDHGB6 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
19 NC_000005.10 5 141463758 - PCDHGB5 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
20 NC_000005.10 5 141463758 - PCDHGB3 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
21 NC_000005.10 5 141463758 - PCDHGB2 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
22 NC_000005.10 5 141463758 - PCDHGB1 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
23 NC_000005.10 5 141463758 - PCDHGA11 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
24 NC_000005.10 5 141463758 - PCDHGA10 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
25 NC_000005.10 5 141463758 - PCDHGA9 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
26 NC_000005.10 5 141463758 - PCDHGA7 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
27 NC_000005.10 5 141463758 - PCDHGA6 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
28 NC_000005.10 5 141463758 - PCDHGA5 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
29 NC_000005.10 5 141463758 - PCDHGA4 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
30 NC_000005.10 5 141463758 - PCDHGA3 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
31 NC_000005.10 5 141463758 - PCDHGA2 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
32 NC_000005.10 5 141463758 - PCDHGA1 NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier II
33 NC_000001.11 1 67341023 - IL12RB2 NNNTTCTAAACAATAAGAGA NGT 2 0.014 Tier II
34 NC_000001.11 1 240473876 - FMN2 NNNTTCTAACCAATATGAGA NGG 1 0.0 Tier II
35 NC_000023.11 X 110085568 - TMEM164 NNNTTCTAACCAATATGAGA NAG 2 0.0 Tier II
36 NC_000002.12 2 236486010 + IQCA1 NNNTTCTAACCAATATGATA NGG 2 0.0 Tier II
37 NC_000006.12 6 90032666 - BACH2 NNNTTCTAACCAATGTGAGA NGG 2 0.0 Tier II
38 NC_000011.10 11 117399689 + CEP164 NNNTTCTAACCAATGTGAGA NGG 2 0.0 Tier II
39 NC_000010.11 10 25372167 - GPR158 NNNTTCTAACCAGTATGAGA NGG 2 0.0 Tier II
40 NC_000002.12 2 134263460 + MGAT5 NNNTTCTAACCCAGAAGAGA NGG 2 0.0 Tier II
41 NC_000004.12 4 159739292 - LOC107986324 NNNTTTTAATCAATAAGAGA NGG 2 0.8739 Tier III
42 NC_000004.12 4 159739292 - LINC02233 NNNTTTTAATCAATAAGAGA NGG 2 0.8739 Tier III
43 NC_000003.12 3 106193730 + LOC105374027 NNNTTCTAACCAATAAAAAA NGG 2 0.6667 Tier III
44 NC_000007.14 7 134370828 - LOC105375517 NNNTTCAAGCCAATAAGAGA NGG 2 0.5833 Tier III
45 NC_000007.14 7 134370828 - LOC105375518 NNNTTCAAGCCAATAAGAGA NGG 2 0.5833 Tier III
46 NC_000001.11 1 163283607 + LOC127814295 NNNTTCTAAACAATAAGATA NGG 2 0.5778 Tier III
47 NC_000004.12 4 18694077 - LOC105374510 NNNTTCTAACAAATTAGAGA NGG 2 0.15 Tier III
48 NC_000012.12 12 83459109 - LOC105369874 NNNTTCTAACCAATAGGATA NGG 2 0.1282 Tier III
49 NC_000011.10 11 49670131 - GRM5P1 NNNTTCTAACCACTAAGAGT NGG 2 0.1263 Tier III
50 NC_000015.10 15 93609141 - LOC107983974 NNNTTCTATCCAATTAGAGA NGG 2 0.12 Tier III
51 NC_000003.12 3 35100389 + LOC101928135 NNNTTCTAAGCAATAAGAGA NAG 2 0.1008 Tier III
52 NC_000010.11 10 23557316 - LOC105376454 NNNTTCTCACCAATAAGAGA NGA 2 0.0298 Tier III
53 NC_000005.10 5 141463758 - PCDHG@ NNNTTCTAACCCATAAGAGA NGA 2 0.0183 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 92694985 - Nedd1 NNNTTCTCACCAATCAGAGA NGG 2 0.1169 Tier I
2 NC_000080.6 14 47402996 + Dlgap5 NNNTTCTAATCAATAAAAGA NGG 2 0.8784 Tier II
3 NC_000071.6 5 49329538 + Kcnip4 NNNTTTTAACCAAAAAGAGA NGG 2 0.5748 Tier II
4 NC_000079.6 13 117912457 + Hcn1 NNNTTCAAACCAGTAAGAGA NGG 2 0.5707 Tier II
5 NC_000068.7 2 151495077 - Nsfl1c NNNTTCTGAGCAATAAGAGA NGG 2 0.2852 Tier II
6 NC_000071.6 5 74422962 + Scfd2 NNNTTCAAACTAATAAGAGA NGG 2 0.2692 Tier II
7 NC_000083.6 17 71223709 - Lpin2 NNNTACTAACCAATAAGACA NGG 2 0.2241 Tier II
8 NC_000079.6 13 108787582 - Pde4d NNNTTCTATCCTATAAGAGA NGG 2 0.2 Tier II
9 NC_000086.7 X 74365729 + Ubl4a NNNTTCTGACCAATAAGAGA NAG 2 0.1901 Tier II
10 NC_000085.6 19 40990885 + Blnk NNNTTCTATCCAATAAGAGA NAG 2 0.1556 Tier II
11 NC_000073.6 7 139249571 - Pwwp2b NNNTACTAACCAACAAGAGA NGG 2 0.1429 Tier II
12 NC_000079.6 13 17167224 + Sugct NNNTACTAACCAATAGGAGA NGG 2 0.0962 Tier II
13 NC_000076.6 10 117352493 - Cpsf6 NNNTACTAACCAATAAGAGA NGA 2 0.0347 Tier II
14 NC_000085.6 19 14571259 - Tle4 NNNTTCTAAGCAATATGAGA NGG 2 0.0 Tier II
15 NC_000076.6 10 125921590 - Gm29944 NNNTTCTAACCAATATGTGA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)