Construct: sgRNA BRDN0001149469

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GACCTTCATTGAAGAAAAGC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80257

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 30952316 + PUM1 NNNTTTCATTGAAGACAAGC NGG 2 0.0 Tier I
2 NC_000001.11 1 101842758 + OLFM3 NNNCTCCACTGAAGAAAAGC NGG 2 0.8392 Tier II
3 NC_000015.10 15 82537994 + RPS17 NNNCTTCACAGAAGAAAAGC NGG 2 0.7912 Tier II
4 NC_000012.12 12 5677793 - ANO2 NNNTTCCATTGAAGAAAAGC NGG 2 0.7273 Tier II
5 NC_000009.12 9 120772715 - FBXW2 NNNCTTCATTAAAGAAAAAC NGG 2 0.7143 Tier II
6 NC_000003.12 3 61697149 - PTPRG NNNCTTCAGTGAAGAAAAGC NGG 1 0.619 Tier II
7 NC_000005.10 5 61068647 - NDUFAF2 NNNCTTCACTGAAGAAAATC NGG 2 0.6154 Tier II
8 NC_000005.10 5 134740918 - CAMLG NNNCTGCATTAAAGAAAAGC NGG 2 0.5714 Tier II
9 NC_000013.11 13 75376058 + TBC1D4 NNNCTTCAACGAAGAAAAGC NGG 2 0.4952 Tier II
10 NC_000022.11 22 18130035 - TUBA8 NNNCTTCTTAGAAGAAAAGC NGG 2 0.3673 Tier II
11 NC_000010.11 10 50740714 - ASAH2B NNNCTTGATTGAAAAAAAGC NGG 2 0.3529 Tier II
12 NC_000003.12 3 15424768 - METTL6 NNNCTTTATTCAAGAAAAGC NGG 2 0.3482 Tier II
13 NC_000004.12 4 150938521 - LRBA NNNTTTCATTCAAGAAAAGC NGG 2 0.3429 Tier II
14 NC_000006.12 6 155238850 + TIAM2 NNNCTTCTTTGGAGAAAAGC NGG 2 0.3095 Tier II
15 NC_000006.12 6 155238850 + TFB1M NNNCTTCTTTGGAGAAAAGC NGG 2 0.3095 Tier II
16 NC_000015.10 15 52783753 - ONECUT1 NNNTTTCATTTAAGAAAAGC NGG 2 0.3077 Tier II
17 NC_000001.11 1 29070825 - EPB41 NNNCTTCAATGCAGAAAAGC NGG 2 0.2444 Tier II
18 NC_000011.10 11 14314023 + RRAS2 NNNCTTCATTGTAGAAAATC NGG 2 0.2222 Tier II
19 NC_000017.11 17 32561915 + MYO1D NNNCCTCATTGAAGTAAAGC NGG 2 0.2 Tier II
20 NC_000011.10 11 34932952 + PDHX NNNCTTCATTGAAAAAAAGC NAG 2 0.1944 Tier II
21 NC_000003.12 3 2607699 + CNTN4 NNNCTTCAGTGAATAAAAGC NGG 2 0.1651 Tier II
22 NC_000003.12 3 54928041 - CACNA2D3 NNNCTGCATTGAAGAAAAGC NAG 2 0.1481 Tier II
23 NC_000003.12 3 54928041 - LRTM1 NNNCTGCATTGAAGAAAAGC NAG 2 0.1481 Tier II
24 NC_000003.12 3 161017884 - PPM1L NNNCTTCATTGGAGTAAAGC NGG 2 0.1444 Tier II
25 NC_000020.11 20 9679895 - PAK5 NNNCTTCATGGAAGAAAAGC NAG 2 0.1296 Tier II
26 NC_000001.11 1 43877498 + ST3GAL3 NNNCTTCATTGAAGAAAAGA NAG 2 0.1296 Tier II
27 NC_000008.11 8 120548544 - SNTB1 NNNCTTCATTCAAGAAAAGT NGG 2 0.1286 Tier II
28 NC_000023.11 X 29227367 - IL1RAPL1 NNNCTTCATTCATGAAAAGC NGG 2 0.1286 Tier II
29 NC_000009.12 9 95295448 - FANCC NNNCTTCTTTGAAGAAAAGT NGG 2 0.1286 Tier II
30 NC_000001.11 1 175000552 + CACYBP NNNCTTCATTCAAGCAAAGC NGG 2 0.1169 Tier II
31 NC_000009.12 9 83285337 - FRMD3 NNNCTTTATTGAAGAATAGC NGG 2 0.1083 Tier II
32 NC_000015.10 15 42637637 + STARD9 NNNCTTCATTGCATAAAAGC NGG 2 0.0702 Tier II
33 NC_000005.10 5 156526890 - SGCD NNNCTTCATTTAAGAAAAGC NCG 2 0.0412 Tier II
34 NC_000011.10 11 31210186 - DCDC1 NNNCTTCATTCAAGAAAAGC NGA 2 0.0298 Tier II
35 NC_000007.14 7 18962285 + HDAC9 NNNCTTCATTGAAGAAAAGC NGC 1 0.0222 Tier II
36 NC_000014.9 14 96280269 - ATG2B NNNCTTCATTTAAGAAAAGC NTG 2 0.015 Tier II
37 NC_000016.10 16 74452452 - GLG1 NNNCTTCATTGAAGCAAAGC NTG 2 0.0106 Tier II
38 NC_000017.11 17 28134983 + NLK NNNCTTCATTGAAGAAAAGG NGA 2 0.0041 Tier II
39 NC_000004.12 4 23743758 - LOC105374528 NNNCTTTAATGAAGAAAAGC NGG 2 0.7545 Tier III
40 NC_000006.12 6 167172578 + TCP10L2 NNNCTTCATTAAAGAAAATC NGG 2 0.6667 Tier III
41 NC_000013.11 13 85365816 - LINC00351 NNNTTTCATTGAAGAAAATC NGG 2 0.5333 Tier III
42 NC_000003.12 3 138045036 - LOC105374128 NNNCTTCAATGAAGAAATGC NGG 2 0.4643 Tier III
43 NC_000014.9 14 19286003 - LINC01297-DUXAP10-NBEAP6 NNNCTTCATAGAAGAAAAGA NGG 2 0.4286 Tier III
44 NC_000018.10 18 79001403 + LOC105372225 NNNCTTCATTGAAAAAAAGA NGG 2 0.375 Tier III
45 NC_000010.11 10 43345116 + LOC105378271 NNNCTTTATTCAAGAAAAGC NGG 2 0.3482 Tier III
46 NC_000010.11 10 43345116 + LOC107984226 NNNCTTTATTCAAGAAAAGC NGG 2 0.3482 Tier III
47 NC_000009.12 9 83285337 - LOC124902190 NNNCTTTATTGAAGAATAGC NGG 2 0.1083 Tier III
48 NC_000018.10 18 2052379 - LOC105371956 NNNCTTCACTGAAGAAAAGG NGG 2 0.0543 Tier III
49 NC_000018.10 18 73220044 + LINC02864 NNNCTTCATTGAAGAAAAGC NTG 1 0.039 Tier III
50 NC_000002.12 2 107799421 + GACAT1 NNNCTTCATTGAAGAAAAGA NGC 2 0.0111 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 94953877 - Mysm1 NNNTTTCAGTGAAGAAAAGC NGG 2 0.4952 Tier I
2 NC_000083.6 17 45507057 - Aars2 NNNCTGCATTGACGAAAAGC NGG 2 0.1203 Tier I
3 NC_000070.6 4 130764113 - Pum1 NNNTTTCATTGAAGACAAGC NGG 2 0.0 Tier I
4 NC_000077.6 11 54529298 + Rapgef6 NNNCTTTATTAAAGAAAAGC NGG 2 0.8125 Tier II
5 NC_000077.6 11 82750684 + Cct6b NNNCTTCACAGAAGAAAAGC NGG 2 0.7912 Tier II
6 NC_000080.6 14 31099920 - Smim4 NNNCTTCACAGAAGAAAAGC NGG 2 0.7912 Tier II
7 NC_000080.6 14 31099920 - Pbrm1 NNNCTTCACAGAAGAAAAGC NGG 2 0.7912 Tier II
8 NC_000077.6 11 78682541 + Nlk NNNATTCACTGAAGAAAAGC NGG 2 0.7773 Tier II
9 NC_000086.7 X 60075639 - Mcf2 NNNCTTCATTAAAGAAAAGA NGG 2 0.5 Tier II
10 NC_000080.6 14 61419524 - Kpna3 NNNCTTCATTGAAGGAAATC NGG 2 0.4333 Tier II
11 NC_000085.6 19 40316141 + Sorbs1 NNNCTTCAATGAAGAAAACC NGG 2 0.4163 Tier II
12 NC_000071.6 5 140122034 + Mad1l1 NNNCTTCACTGAAGAAAACC NGG 2 0.4138 Tier II
13 NC_000069.6 3 129993524 - Sec24b NNNGTTTATTGAAGAAAAGC NGG 2 0.4063 Tier II
14 NC_000067.6 1 132438374 + Dstyk NNNCATCATTGAAGAAAATC NGG 2 0.3333 Tier II
15 NC_000077.6 11 40727073 + Hmmr NNNCATCATTGAAGAAAATC NGG 2 0.3333 Tier II
16 NC_000082.6 16 38979805 - Igsf11 NNNCTTTATTGAAGAAAGGC NGG 2 0.325 Tier II
17 NC_000082.6 16 46039582 - Cd96 NNNCTTTATTGAAGAAAGGC NGG 2 0.325 Tier II
18 NC_000070.6 4 76424391 - Ptprd NNNCTTATTTGAAGAAAAGC NGG 2 0.3214 Tier II
19 NC_000085.6 19 61090072 - Grk5 NNNCTTCCTTGGAGAAAAGC NGG 2 0.3095 Tier II
20 NC_000085.6 19 61090072 - Zfp950 NNNCTTCCTTGGAGAAAAGC NGG 2 0.3095 Tier II
21 NC_000077.6 11 50745770 - Adamts2 NNNCTTCATTTAAGAAAAAC NGG 2 0.2747 Tier II
22 NC_000067.6 1 157510413 - Sec16b NNNCTTTATTGTAGAAAAGC NGG 2 0.2708 Tier II
23 NC_000068.7 2 16232035 + Malrd1 NNNCTTTATTGTAGAAAAGC NGG 2 0.2708 Tier II
24 NC_000069.6 3 87666226 + Arhgef11 NNNCTTTATTGTAGAAAAGC NGG 2 0.2708 Tier II
25 NC_000069.6 3 114951032 - Olfm3 NNNCTTTATTGTAGAAAAGC NGG 2 0.2708 Tier II
26 NC_000071.6 5 150363794 + Fry NNNCTTTATTGTAGAAAAGC NGG 2 0.2708 Tier II
27 NC_000073.6 7 96979093 - Nars2 NNNCTTTATTGTAGAAAAGC NGG 2 0.2708 Tier II
28 NC_000076.6 10 64358304 - Ctnna3 NNNCTTTATTGTAGAAAAGC NGG 2 0.2708 Tier II
29 NC_000077.6 11 103631713 + Lyzl6 NNNCTTTATTGTAGAAAAGC NGG 2 0.2708 Tier II
30 NC_000079.6 13 20895188 - Aoah NNNCTTTATTGTAGAAAAGC NGG 2 0.2708 Tier II
31 NC_000067.6 1 184967535 + Mark1 NNNCTTCACTGAAGCAAAGC NGG 2 0.2517 Tier II
32 NC_000071.6 5 112990312 - Grk3 NNNCTTTATTGAAGAAAAGC NAG 2 0.2106 Tier II
33 NC_000068.7 2 162083280 - Ptprt NNNCTTCATTGAAGAAAATT NGG 2 0.2 Tier II
34 NC_000086.7 X 141303452 - Col4a6 NNNCTTCATTGAAAAAAAGC NAG 2 0.1944 Tier II
35 NC_000081.6 15 55647638 - Sntb1 NNNCTTCATTGAAGAGAAAC NGG 2 0.1374 Tier II
36 NC_000067.6 1 32465602 - Khdrbs2 NNNCTTCATTGTAGAAAGGC NGG 2 0.1333 Tier II
37 NC_000068.7 2 38042402 - Dennd1a NNNCTTCATTGTAGAAAGGC NGG 2 0.1333 Tier II
38 NC_000072.6 6 47802147 - Pdia4 NNNCTTCATTGTAGAAAGGC NGG 2 0.1333 Tier II
39 NC_000083.6 17 32257901 - Brd4 NNNCTTCATTGTAGAAAGGC NGG 2 0.1333 Tier II
40 NC_000067.6 1 160782511 + Rabgap1l NNNCTTCATTGCAGAAAAGA NGG 2 0.1316 Tier II
41 NC_000077.6 11 36522245 - Tenm2 NNNCTTCATTGAAGAAATGC NAG 2 0.1296 Tier II
42 NC_000075.6 9 15484087 - Smco4 NNNCTGCATTGAAGAACAGC NGG 2 0.1008 Tier II
43 NC_000075.6 9 65008294 + Hacd3 NNNCTTCATGGAAGTAAAGC NGG 2 0.1 Tier II
44 NC_000077.6 11 118212193 + Cyth1 NNNCTTCATTTAAGAAAAGC NAG 2 0.0997 Tier II
45 NC_000070.6 4 147474731 + Zfp982 NNNCATCATTGAAGAACAGC NGG 2 0.0882 Tier II
46 NC_000070.6 4 9340157 - Clvs1 NNNCTTCATTGATGCAAAGC NGG 2 0.0818 Tier II
47 NC_000084.6 18 12749762 - Cabyr NNNCTCCATTGAAGAAAAGC NGA 2 0.0631 Tier II
48 NC_000086.7 X 57391930 - Rbmx NNNCTTCATTGACTAAAAGC NGG 2 0.0561 Tier II
49 NC_000084.6 18 74203806 - Ska1 NNNCCTCATTGAAGAAAAGC NTG 2 0.039 Tier II
50 NC_000082.6 16 11235542 + Gspt1 NNNCTTCATTGAAGAAAAAC NGC 2 0.0159 Tier II
51 NC_000078.6 12 34087343 - Hdac9 NNNCTTCATTGAGGAAAAGC NGT 2 0.0105 Tier II
52 NC_000070.6 4 86042469 + Adamtsl1 NNNCTTCATTGAAGAACAGG NGG 2 0.0104 Tier II
53 NC_000069.6 3 28409440 - Tnik NNNCTTCATTGTAGAAAAGC NGC 2 0.0074 Tier II
54 NC_000076.6 10 20310798 + Bclaf1 NNNCTTCATTTAAGAAAAGC NGT 2 0.0062 Tier II
55 NC_000073.6 7 76925561 - Agbl1 NNNCTTCATTGAAGATAAGA NGG 2 0.0 Tier II
56 NC_000080.6 14 24799544 - Gm31838 NNNCATCATTGAAGAAAAGC NGG 1 0.5 Tier III
57 NC_000072.6 6 134339439 - 4933406J09Rik NNNCTGCATTGGAGAAAAGC NGG 2 0.4127 Tier III
58 NC_000085.6 19 23013419 + C330002G04Rik NNNCATCATTGAAGAAAATC NGG 2 0.3333 Tier III
59 NC_000071.6 5 23389052 + 5031425E22Rik NNNGTTCATTGAAGAAAATC NGG 2 0.3333 Tier III
60 NC_000082.6 16 38979805 - Gm34258 NNNCTTTATTGAAGAAAGGC NGG 2 0.325 Tier III
61 NC_000086.7 X 147722682 + Gm16400 NNNCTTCATTGATGAAAAGC NGG 1 0.3 Tier III
62 NC_000072.6 6 3342661 + Gm20559 NNNCTTCATTTAAAAAAAGC NGG 2 0.2885 Tier III
63 NC_000067.6 1 157510413 - Gm15486 NNNCTTTATTGTAGAAAAGC NGG 2 0.2708 Tier III
64 NC_000068.7 2 4913915 - Gm46728 NNNCTTTATTGTAGAAAAGC NGG 2 0.2708 Tier III
65 NC_000071.6 5 111473297 - Gm42154 NNNCTACATTGATGAAAAGC NGG 2 0.26 Tier III
66 NC_000082.6 16 73344697 - 4930500H12Rik NNNCATCATTGAAGAAATGC NGG 2 0.25 Tier III
67 NC_000077.6 11 14590052 - 1700046C09Rik NNNCTTCATTGAAGAAAAAT NGG 2 0.2143 Tier III
68 NC_000081.6 15 22000182 + Gm41278 NNNCTTCATTGTAGAAAGGC NGG 2 0.1333 Tier III
69 NC_000082.6 16 22880377 + Gm30505 NNNCTTCATTTATGAAAAGC NGG 2 0.1154 Tier III
70 NC_000083.6 17 51882793 + Gm20098 NNNCTTCCTTGAAGAAAAGC NTG 2 0.0167 Tier III
71 NC_000085.6 19 25895704 + Gm34516 NNNATTCATTGAAGAAAAGC NGT 2 0.0136 Tier III
72 NC_000078.6 12 34087343 - Gm40384 NNNCTTCATTGAGGAAAAGC NGT 2 0.0105 Tier III
73 NC_000070.6 4 9803379 + 4930412C18Rik NNNCTCCATTGAAGACAAGC NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)