Construct: ORF TRCN0000465232

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003842.1_s317c1
Derived from:
ccsbBroadEn_11008
DNA Barcode:
CTCGCCGGGAATAAGATTTCCTCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ODF2 (4957)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465232
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4957 ODF2 outer dense fiber of sperm ... NM_001351586.2 94.7% 94.7% 1_126del;639A>G
2 human 4957 ODF2 outer dense fiber of sperm ... NM_002540.5 94.7% 94.7% 1_126del;639A>G
3 human 4957 ODF2 outer dense fiber of sperm ... NM_001351588.2 93.1% 87.7% (many diffs)
4 human 4957 ODF2 outer dense fiber of sperm ... NM_001242352.1 92.5% 92.5% 1_126del;176_232del
5 human 4957 ODF2 outer dense fiber of sperm ... NM_001351584.2 92.5% 92.5% 1_126del;176_232del;696A>G
6 human 4957 ODF2 outer dense fiber of sperm ... NM_001351585.2 92.5% 92.5% 1_126del;176_232del;696A>G
7 human 4957 ODF2 outer dense fiber of sperm ... NM_001242353.2 91.9% 92% 1_141del;191_247del;711A>G
8 human 4957 ODF2 outer dense fiber of sperm ... NM_153433.2 91.9% 92% 1_141del;191_247del;711A>G
9 human 4957 ODF2 outer dense fiber of sperm ... NM_001351583.2 88.9% 89% 1_126del;639A>G;1940_2095del
10 human 4957 ODF2 outer dense fiber of sperm ... NM_001351587.2 88.9% 88.8% (many diffs)
11 human 4957 ODF2 outer dense fiber of sperm ... NM_001351582.2 87.8% 87.9% 1_258del;308_364del;828A>G
12 human 4957 ODF2 outer dense fiber of sperm ... NM_001351581.1 87.3% 87.3% 1_273del;323_379del
13 human 4957 ODF2 outer dense fiber of sperm ... NM_001351579.2 87% 87.1% (many diffs)
14 human 4957 ODF2 outer dense fiber of sperm ... NM_001351580.2 87% 87.1% (many diffs)
15 human 4957 ODF2 outer dense fiber of sperm ... NM_153435.1 85.4% 85.4% 1_390del
16 human 4957 ODF2 outer dense fiber of sperm ... NM_001351578.1 83.6% 83.6% 1_390del;440_496del
17 human 4957 ODF2 outer dense fiber of sperm ... NM_001351577.1 79.1% 79.1% 1_390del;440_496del;2261_2416del
18 human 4957 ODF2 outer dense fiber of sperm ... NM_153437.2 72.5% 70.7% (many diffs)
19 human 4957 ODF2 outer dense fiber of sperm ... NM_153440.1 72.5% 70.7% (many diffs)
20 human 4957 ODF2 outer dense fiber of sperm ... NM_153436.1 70.8% 69.1% (many diffs)
21 human 4957 ODF2 outer dense fiber of sperm ... NM_001242354.1 70.5% 64.1% (many diffs)
22 human 4957 ODF2 outer dense fiber of sperm ... NM_153432.1 67.2% 65.6% (many diffs)
23 human 4957 ODF2 outer dense fiber of sperm ... NM_153439.1 65.3% 63.7% (many diffs)
24 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316304.1 85.6% 92.1% (many diffs)
25 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316300.1 85.5% 92% (many diffs)
26 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316301.1 85.5% 92% (many diffs)
27 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316299.1 85% 91.6% (many diffs)
28 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316294.1 84.9% 91.5% (many diffs)
29 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316295.1 84.9% 91.5% (many diffs)
30 mouse 18286 Odf2 outer dense fiber of sperm ... NM_001113213.1 83.6% 90% (many diffs)
31 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316293.1 83.6% 90% (many diffs)
32 mouse 18286 Odf2 outer dense fiber of sperm ... NM_001177659.1 83.5% 89.9% (many diffs)
33 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316290.1 83.5% 89.9% (many diffs)
34 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316291.1 83.5% 89.9% (many diffs)
35 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316292.1 83.5% 89.9% (many diffs)
36 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316289.1 83.1% 89.5% (many diffs)
37 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316280.1 83% 89.4% (many diffs)
38 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316281.1 83% 89.4% (many diffs)
39 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316283.1 83% 89.4% (many diffs)
40 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316284.1 83% 89.4% (many diffs)
41 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316285.1 83% 89.4% (many diffs)
42 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316286.1 83% 89.4% (many diffs)
43 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316288.1 83% 89.4% (many diffs)
44 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316279.1 82.9% 89.3% (many diffs)
45 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316276.1 80.7% 86.9% (many diffs)
46 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316275.1 80.6% 86.7% (many diffs)
47 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316273.1 78.9% 85% (many diffs)
48 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316272.1 78.8% 84.9% (many diffs)
49 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316306.1 66.1% 70.3% (many diffs)
50 mouse 18286 Odf2 outer dense fiber of sperm ... NM_013615.3 65.7% 69.9% (many diffs)
51 mouse 18286 Odf2 outer dense fiber of sperm ... NM_001113214.1 64.6% 68.7% (many diffs)
52 mouse 18286 Odf2 outer dense fiber of sperm ... XM_017316305.1 64.6% 68.7% (many diffs)
53 mouse 18286 Odf2 outer dense fiber of sperm ... NM_001177661.1 60.9% 64.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2355
ORF length:
2289
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa aggggacact gtgaatgtgc ggcggagtgt ccgggtgaaa accaagaatc
121cacctcattg cctggagatc acgccaccat cttcagaaaa gctggtctca gtgatgcggt
181taagtgacct ctctacagaa gatgatgact caggtcactg taaaatgaac cgttatgata
241agaagattga tagtctaatg aatgcggttg gttgtctgaa gtctgaggtc aagatgcaaa
301aaggtgagcg ccagatggcc aaaaggttcc tggaggaacg gaaggaagag ctggaggagg
361tggcccacga actggctgag actgagcacg agaacacggt gttgaggcac aacatcgagc
421gcatgaagga ggagaaggac ttcaccatac ttcagaagaa acacctacaa caggagaagg
481agtgcctcat gtccaagctg gtggaggcgg aaatggatgg ggctgcggct gccaagcagg
541tcatggcctt gaaggatacc atcgggaagc tgaaaacgga gaaacaaatg acctgcacgg
601acatcaacac cctgacaagg cagaaggaac ttctcctgca gaagctgagc acatttgagg
661agaccaaccg caccctccga gacctcctga gggaacagca ctgcaaagag gattctgaaa
721gactaatgga gcaacaagga gcactgctga aacggctggc ggaggccgac tcagagaaag
781cgcgcctgct gttactgctg caagacaagg acaaggaggt ggaagagctc cttcaggaaa
841tacaatgtga gaaggctcaa gcaaagacag cctctgagct ttctaaatcc atggagtcca
901tgcgtgggca tttgcaggca cagcttcggt ccaaagaggc tgagaacagt cgcctgtgca
961tgcagattaa gaatctggag cgcagcggga atcagcataa ggcagaagtg gaggccatca
1021tggagcagct gaaggagttg aagcagaagg gagaccgaga caaagagagc ttgaagaagg
1081ccatccgagc ccagaaggag cgagccgaga agagcgagga gtatgctgag cagctacacg
1141tgcaactcgc tgacaaggat ctttatgtcg ctgaagcttt atccactctg gaatcctgga
1201ggagccgcta caaccaagtt gtaaaagaaa agggagacct tgagctggaa attattgtcc
1261tgaatgaccg ggtaacagat cttgtaaacc aacaacaaac cctggaggag aagatgcggg
1321aagaccggga tagcctggtg gagagactac accgtcagac tgctgagtat tccgcattca
1381agctggagaa tgagaggctg aaggccagct ttgctccaat ggaggacaaa ctcaaccagg
1441cacacctcga ggtccagcag ctgaaggcct cagtgaagaa ctatgagggg atgattgaca
1501actataagag tcaggtgatg aagaccagat tggaggctga tgaagtagct gcccagctag
1561aacgctgtga caaagagaac aagatcctta aagatgagat gaacaaagag attgaggcgg
1621cacgaaggca gttccagtct cagctggctg acctgcagca gctccctgac atcctgaaga
1681tcacggaggc gaagctggct gagtgccaag accaactgca gggctatgag cggaagaaca
1741tcgacctcac agccatcata tcagacctgc gcagccggat cgaacaccag ggggacaagc
1801tggagatggc gagagagaaa catcaggctt cccagaagga aaataaacag ctgagtctga
1861aggtggatga actggagagg aaactggagg cgaccagtgc ccagaatatc gagttcctac
1921aggtgattgc caagagggag gaggcaatcc accagtccca gctgcggctg gaggagaaaa
1981cacgggaatg tgggaccctg gcaaggcagt tggagagtgc cattGAAGAT GCGAGGAGGC
2041AGGTGGAACA AACCAAGGAG CACGCACTCT CCAAGGAGCG AGCAGCCCAG AACAAAATCC
2101TGGACCTTGA GACCCAGCTG AGCAGAACCA AAACGGAATT GAGCCAGCTG CGGCGGAGCC
2161GTGATGATGC GGACCGCCGC TACCAGAGCC GGCTGCAAGA CCTGAAAGAT CGCCTGGAGC
2221AGTCCGAGAG CACCAACCGC AGCATGCAGA ACTACGTCCA GTTCCTCAAA TCATCATACG
2281CCAACGTGTT TGGGGATGGT CCCTATTCCA CCTTCCTGAC TAGCTCTCCC ATCCGCTCCC
2341GATCTCCTCC TGCCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
2401CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
2461TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTCGCCGGGA ATAAGATTTC
2521CTCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)