Construct: ORF TRCN0000465242
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003494.1_s317c1
- Derived from:
- ccsbBroadEn_13209
- DNA Barcode:
- AACCCCGAAGTTTGTATTGGCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIRPA (140885)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465242
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140885 | SIRPA | signal regulatory protein a... | NM_001330728.1 | 98.4% | 97.4% | (many diffs) |
| 2 | human | 140885 | SIRPA | signal regulatory protein a... | XM_005260670.3 | 98.4% | 97.4% | (many diffs) |
| 3 | human | 140885 | SIRPA | signal regulatory protein a... | XM_024451836.1 | 98.4% | 97.4% | (many diffs) |
| 4 | human | 140885 | SIRPA | signal regulatory protein a... | NM_001040022.1 | 97.7% | 96.6% | (many diffs) |
| 5 | human | 140885 | SIRPA | signal regulatory protein a... | NM_001040023.2 | 97.7% | 96.6% | (many diffs) |
| 6 | human | 140885 | SIRPA | signal regulatory protein a... | NM_080792.3 | 97.7% | 96.6% | (many diffs) |
| 7 | human | 140885 | SIRPA | signal regulatory protein a... | XM_011529173.2 | 94.6% | 90.2% | (many diffs) |
| 8 | human | 10326 | SIRPB1 | signal regulatory protein b... | NM_001135844.4 | 70.4% | 64.5% | (many diffs) |
| 9 | human | 10326 | SIRPB1 | signal regulatory protein b... | NM_001329157.2 | 70.4% | 64.5% | (many diffs) |
| 10 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_011529134.2 | 69.4% | 64.5% | (many diffs) |
| 11 | human | 10326 | SIRPB1 | signal regulatory protein b... | XR_001754121.1 | 69% | (many diffs) | |
| 12 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_005260641.3 | 67.2% | 59.2% | (many diffs) |
| 13 | human | 10326 | SIRPB1 | signal regulatory protein b... | NM_006065.4 | 67% | 59% | (many diffs) |
| 14 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_017027577.1 | 67% | 59% | (many diffs) |
| 15 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_017027578.1 | 66.6% | 59.2% | (many diffs) |
| 16 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_017027579.1 | 66.6% | 59.2% | (many diffs) |
| 17 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_017027580.1 | 66.6% | 59.2% | (many diffs) |
| 18 | human | 55423 | SIRPG | signal regulatory protein g... | NM_018556.4 | 65.3% | 53.9% | (many diffs) |
| 19 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_017027583.1 | 63.8% | 58% | (many diffs) |
| 20 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_017027584.1 | 63.8% | 58% | (many diffs) |
| 21 | human | 55423 | SIRPG | signal regulatory protein g... | XM_011529286.2 | 60.9% | 51.9% | (many diffs) |
| 22 | human | 140885 | SIRPA | signal regulatory protein a... | XM_006723545.4 | 55.7% | 54.7% | (many diffs) |
| 23 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_005260643.3 | 48.2% | 41.7% | (many diffs) |
| 24 | human | 55423 | SIRPG | signal regulatory protein g... | NM_001039508.1 | 45.2% | 35.7% | (many diffs) |
| 25 | human | 55423 | SIRPG | signal regulatory protein g... | XM_011529287.2 | 44.4% | 37% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1587
- ORF length:
- 1521
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcccgccggc ccggcccccg gccgcctcgg gccgctgctc tgcctgctgc 121 tcgccgcgtc ctgcgcctgg tcaggagtgg cgggtgagga ggagctgcag gtgattcagc 181 ctgacaagtc cgtatcagtt gcagctggag agtcggccat tctgcactgc actgtgacct 241 ccctgatccc tgtggggccc atccagtggt tcagaggagc tggaccagcc cgggaattaa 301 tctacaatca aaaagaaggc cacttccccc gggtaacaac tgtttcagag tccacaaaga 361 gagaaaacat ggacttttcc atcagcatca gtaacatcac cccagcagat gccggcacct 421 actactgtgt gaagttccgg aaagggagcc ctgacacgga gtttaagtct ggagcaggca 481 ctgagctgtc tgtgcgtgcc aaaccctctg cccccgtggt atcgggccct gcggcgaggg 541 ccacacctca gcacacagtg agcttcacct gcgagtccca cggcttctca cccagagaca 601 tcaccctgaa atggttcaaa aatgggaatg agctctcaga cttccagacc aacgtggacc 661 ccgtaggaga gagcgtgtcc tacagcatcc acagcacagc caaggtggtg ctgacccgcg 721 aggacgttca ctctcaagtc atctgcgagg tggcccacgt caccttgcag ggggaccctc 781 ttcgtgggac tgccaacttg tctgagacca tccgagttcc acccaccttg gaggttactc 841 aacagcccgt gagggcagag aaccaggtga atgtcacctg ccaggtgagg aagttctacc 901 cccagagact acagctgacc tggttggaga atggaaacgt gtcccggaca gaaacggcct 961 caaccgttac agagaacaag gatggtacct acaactggat gagctggctc ctggtgaatg 1021 tatctgccca cagggatgat gtgaagctca cctgccaggt ggagcatgac gggcagccag 1081 cggtcagcaa aagccatgac ctgaaggtct cagcccaccc gaaggagcag ggctcaaata 1141 ccgccgctga gaacactgga tctaatgaac ggaacatcta tattgtggtg ggtgtggtgt 1201 gcaccttgct ggtggcccta ctgatggcgg ccctctaccT CGTCCGAATC AGACAGAAGA 1261 AAGCCCAGGG CTCCACTTCT TCTACAAGGT TGCATGAGCC CGAGAAGAAT GCCAGAGAAA 1321 TAACACAGGT ACAGTCCTTG GACACAAATG ATATCACATA TGCAGACCTG AACCTGCCCA 1381 AGGGGAAGAA GCCTGCTCCC CAGGCTGCGG AGCCCAACAA CCACACGGAG TATGCCAGCA 1441 TTCAGACCAG CCCGCAGCCC GCGTCGGAGG ACACCCTCAC CTATGCTGAC CTGGACATGG 1501 TCCACCTCAA CCGGACCCCC AAGCAGCCGG CCCCCAAGCC TGAGCCGTCC TTCTCAGAGT 1561 ACGCCAGCGT CCAGGTCCCG AGGAAGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1621 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1681 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACCCCGA 1741 AGTTTGTATT GGCTTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1801 aagatt