Construct: ORF TRCN0000465288
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001870.1_s317c1
- Derived from:
- ccsbBroadEn_12619
- DNA Barcode:
- TCGGGGTTTTAGCGGAATGCCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HHIPL2 (79802)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465288
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79802 | HHIPL2 | HHIP like 2 | NM_024746.4 | 73% | 73% | 1_585del |
| 2 | human | 79802 | HHIPL2 | HHIP like 2 | XM_024449814.1 | 71.1% | 71.1% | 1_585del;1804_1860del |
| 3 | human | 79802 | HHIPL2 | HHIP like 2 | XM_011509986.1 | 57.1% | 54.6% | (many diffs) |
| 4 | human | 79802 | HHIPL2 | HHIP like 2 | XM_017002350.1 | 40% | 39.9% | (many diffs) |
| 5 | mouse | 78772 | Hhipl2 | hedgehog interacting protei... | NM_001308052.1 | 79.5% | 78.4% | (many diffs) |
| 6 | mouse | 78772 | Hhipl2 | hedgehog interacting protei... | XM_011249736.2 | 78.6% | 77.5% | (many diffs) |
| 7 | mouse | 78772 | Hhipl2 | hedgehog interacting protei... | XM_011249737.2 | 78.6% | 77.5% | (many diffs) |
| 8 | mouse | 78772 | Hhipl2 | hedgehog interacting protei... | XM_017312944.1 | 78.6% | 77.5% | (many diffs) |
| 9 | mouse | 78772 | Hhipl2 | hedgehog interacting protei... | XM_011249734.2 | 68.6% | 67.7% | (many diffs) |
| 10 | mouse | 78772 | Hhipl2 | hedgehog interacting protei... | XM_011249735.2 | 68.6% | 67.7% | (many diffs) |
| 11 | mouse | 78772 | Hhipl2 | hedgehog interacting protei... | NM_030175.5 | 61.3% | 60.4% | (many diffs) |
| 12 | mouse | 78772 | Hhipl2 | hedgehog interacting protei... | XM_011249733.2 | 60.8% | 59.9% | (many diffs) |
| 13 | mouse | 78772 | Hhipl2 | hedgehog interacting protei... | XR_880811.1 | 46.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1653
- ORF length:
- 1587
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggcccaagat cctcagggct gcctgcagct ctgcctgagc gaggtggcca 121 acgggctgag gaaccccgtc tccatggtcc atgctgggga cggcacccat cgcttctttg 181 ttgccgagca ggtaggagtg gtgtgggtct acctccctga tgggagtcgc ctggagcaac 241 ccttcctgga cctcaagaac atcgtgttga ccaccccatg gatcggggat gagagaggct 301 tcttggggtt ggcttttcac cccaaattcc gccacaatcg caagttctat atttattatt 361 cgtgcctgga caagaagaag gtagaaaaga tccgaattag tgagatgaag gtttctcggg 421 ctgatcctaa caaagctgac ctgaaatcag agagggtcat cttggagatt gaagaaccag 481 cctcaaacca taatggcgga caacttcttt ttggcctgga tggctatatg tacatattca 541 ctggggacgg gggacaggct ggagatccct ttggcctgtt tggaaatgct cagaacaaaa 601 gttccctgct gggaaaagtt ttaaggatcg atgtgaacag ggcaggctca catggcaagc 661 ggtaccgagt cccctcggac aatccatttg tttctgagcc aggggcccac cccgccatct 721 atgcctatgg gatcaggaac atgtggcgtt gtgctgtgga ccgaggggac cccatcacgc 781 gccagggccg aggccggata ttctgtgggg acgtgggcca gaacaggttt gaagaggttg 841 acctcatttt gaaaggtgga aactatggct ggagagcaaa ggaagggttt gcatgttatg 901 acaaaaaact ttgtcacaat gcctctttgg atgatgttct gccaatctat gcttatggcc 961 atgcagtggg gaagtcagtc actggaggtt atgtctatcg tggttgtgaa tccccaaatc 1021 tcaatggcct gtatatcttt ggagacttca tgagtggtcg acttatggct ttgcaggaag 1081 atagaaaaaa caagaaatgg aagaagcagg atctttgcct gggcagcacc acgtcctgtg 1141 ccttcccagg gctgatcagc acccatagca agttcatcat ctcctttgct gaagatgaag 1201 caggggagct gtatttcctg gcgacctctt acccaagtgc ctatgcacca cgtggatcta 1261 tttacaagtt tgttgacccc tcaaggcgag cacccCCAGG CAAGTGCAAA TACAAGCCAG 1321 TGCCCGTGAG AACCAAGAGT AAGCGGATCC CGTTCAGACC ACTCGCCAAG ACAGTCTTGG 1381 ACTTGCTAAA GGAACAATCA GAGAAAGCTG CTAGAAAATC TTCCAGTGCA ACCTTAGCTT 1441 CTGGCCCAGC CCAGGGTTTG TCTGAGAAAG GCTCCTCCAA GAAGCTGGCT TCTCCTACAA 1501 GCAGCAAGAA TACATTGCGA GGGCCTGGTA CAAAGAAGAA AGCCAGAGTG GGGCCCCACG 1561 TCCGCCAGGG CAAGAGGAGG AAGAGCCTGA AAAGCCACAG TGGCAGGATG AGGCCATCAG 1621 CAGAGCAGAA GCGAGCTGGC AGAAGTCTCC CTTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1681 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1741 GTCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCG 1801 GGGTTTTAGC GGAATGCCGC GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 1861 tgtgaaagat t