Construct: ORF TRCN0000465307
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011571.1_s317c1
- Derived from:
- ccsbBroadEn_14064
- DNA Barcode:
- CCGACAAATGACTTCGTTAGGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDT4 (11163)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465307
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11163 | NUDT4 | nudix hydrolase 4 | NM_019094.6 | 100% | 100% | |
| 2 | human | 440672 | NUDT4B | nudix hydrolase 4B | NM_001355407.1 | 99.4% | 99.4% | 254_256delAGC |
| 3 | human | 11163 | NUDT4 | nudix hydrolase 4 | NM_199040.4 | 99.4% | 99.4% | 254_256delAGC |
| 4 | human | 170685 | NUDT10 | nudix hydrolase 10 | NM_001304963.2 | 72.9% | 81.2% | (many diffs) |
| 5 | human | 170685 | NUDT10 | nudix hydrolase 10 | NM_153183.3 | 72.9% | 81.2% | (many diffs) |
| 6 | human | 55190 | NUDT11 | nudix hydrolase 11 | NM_018159.4 | 72.7% | 81.7% | (many diffs) |
| 7 | human | 11163 | NUDT4 | nudix hydrolase 4 | NM_001301023.2 | 71.1% | 71.1% | 0_1ins156 |
| 8 | human | 11163 | NUDT4 | nudix hydrolase 4 | NM_001301024.2 | 71.1% | 71.1% | 0_1ins156 |
| 9 | human | 11163 | NUDT4 | nudix hydrolase 4 | XM_011537795.2 | 71.1% | 71.1% | 0_1ins156 |
| 10 | human | 11163 | NUDT4 | nudix hydrolase 4 | XM_011537796.2 | 71.1% | 71.1% | 0_1ins156 |
| 11 | human | 11163 | NUDT4 | nudix hydrolase 4 | NM_001301022.2 | 70.7% | 70.7% | 0_1ins156;98_100delAGC |
| 12 | human | 11163 | NUDT4 | nudix hydrolase 4 | XM_011537794.2 | 70.7% | 70.7% | 0_1ins156;98_100delAGC |
| 13 | human | 170688 | NUDT4P2 | nudix hydrolase 4 pseudogene 2 | NR_104005.1 | 14% | 1_397del;651_653delAGC;941_3838del | |
| 14 | mouse | 71207 | Nudt4 | nudix (nucleoside diphospha... | NM_027722.4 | 89.8% | 96.1% | (many diffs) |
| 15 | mouse | 71207 | Nudt4 | nudix (nucleoside diphospha... | XM_006514116.2 | 89.3% | 95.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 606
- ORF length:
- 540
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gaagttcaag cccaaccaga cgcggaccta cgaccgcgag ggcttcaaga 121 agcgggcggc gtgcctgtgc ttccggagcg agcaggagga cgaggtgctg ctggtgagta 181 gcagccggta cccagaccag tggattgtcc caggaggagg aatggaaccc gaggaggaac 241 ctggcggtgc tgccgtgagg gaagtttatg aggaggctGG AGTCAAAGGA AAACTAGGCA 301 GACTTCTGGG CATATTTGAG AACCAAGACC GAAAGCACAG AACATATGTT TATGTTCTAA 361 CAGTCACTGA AATATTAGAA GATTGGGAAG ATTCTGTTAA TATTGGAAGG AAGAGAGAGT 421 GGTTCAAAGT AGAAGATGCT ATCAAAGTTC TCCAGTGTCA TAAACCTGTA CATGCAGAGT 481 ATCTGGAAAA GCTAAAGCTG GGTTGTTCCC CAGCCAATGG AAATTCTACA GTCCCTTCCC 541 TTCCGGATAA TAATGCCTTG TTTGTAACCG CTGCACAGAC CTCTGGGTTG CCATCTAGTG 601 TAAGATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 661 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 721 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCGACAAAT GACTTCGTTA GGTGTACGCG 781 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt