Construct: ORF TRCN0000465396
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000496.1_s317c1
- Derived from:
- ccsbBroadEn_12240
- DNA Barcode:
- ATGTGTGAGAATGTTATACCAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC48A1 (55652)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465396
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55652 | SLC48A1 | solute carrier family 48 me... | NM_017842.3 | 60.9% | 60.9% | 1_171del |
| 2 | human | 55652 | SLC48A1 | solute carrier family 48 me... | XM_017019617.2 | 60.9% | 60.9% | 1_171del |
| 3 | human | 55652 | SLC48A1 | solute carrier family 48 me... | XM_005269016.4 | 51.4% | 51.4% | 1_252del |
| 4 | human | 55652 | SLC48A1 | solute carrier family 48 me... | XM_024449045.1 | 48.3% | 48.3% | 1_285del |
| 5 | human | 55652 | SLC48A1 | solute carrier family 48 me... | XM_024449046.1 | 48.3% | 48.3% | 1_285del |
| 6 | mouse | 67739 | Slc48a1 | solute carrier family 48 (h... | NM_026353.4 | 54.3% | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 333
- ORF length:
- 267
- Sequence:
-
1 tcttccattt CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTA CATGCAAGAT TATTGGAGGA CCTGGCTCAA GGGGCTGCGC GGCTTCTTCT 121 TCGTGGGCGT CCTCTTCTCG GCCGTCTCCA TCGCTGCCTT CTGCACCTTC CTCGTGCTGG 181 CCATCACCCG GCATCAGAGC CTCACAGACC CCACCAGCTA CTACCTCTCC AGCGTCTGGA 241 GCTTCATTTC CTTCAAGTGG GCCTTCCTGC TCAGCCTCTA TGCCCACCGC TACCGGGCTG 301 ACTTTGCTGA CATCAGCATC CTCAGCGATT TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 361 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 421 GTCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATG 481 TGTGAGAATG TTATACCAGT CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 541 tgtgaaagat t