Construct: ORF TRCN0000465402
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019018.2_s317c1
- Derived from:
- ccsbBroadEn_14640
- DNA Barcode:
- CTAGCGAGATACATAGTTCGCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGFR2 (2263)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465402
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_022970.3 | 100% | 100% | |
2 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_006717708.3 | 97.5% | 97.5% | 1_57del;1341_1342insGTAACA |
3 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_000141.4 | 96.7% | 96.7% | (many diffs) |
4 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001320658.2 | 96.5% | 96.4% | (many diffs) |
5 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144913.1 | 93.5% | 93.4% | 2304_2305insGA;2307_2307delCins158 |
6 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_006717710.4 | 93.4% | 93.3% | (many diffs) |
7 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_017015920.2 | 93.2% | 93.1% | (many diffs) |
8 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_017015921.2 | 90.1% | 89.9% | (many diffs) |
9 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447890.1 | 87.1% | 87.1% | 1_57del;164_165ins267 |
10 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447888.1 | 86.9% | 86.9% | 1_57del;164_165ins267;1074_1075insGTAACA |
11 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144914.1 | 86.2% | 86.2% | 747_748ins339 |
12 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_023029.2 | 86% | 85.9% | (many diffs) |
13 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144917.2 | 85.7% | 85.7% | 939_940ins351 |
14 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447887.1 | 84.1% | 83.9% | (many diffs) |
15 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447891.1 | 84% | 83.9% | 1_57del;166_167ins345 |
16 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447889.1 | 83.8% | 83.7% | (many diffs) |
17 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144916.1 | 82.9% | 82.6% | (many diffs) |
18 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144919.2 | 82.6% | 82.6% | 107_108ins267;2037_2038insGA;2040_2040delCins158 |
19 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144918.2 | 82.6% | 82.4% | (many diffs) |
20 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001144915.1 | 81.3% | 79.9% | (many diffs) |
21 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_017015924.2 | 81% | 80.7% | (many diffs) |
22 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_017015925.2 | 76.5% | 76.1% | (many diffs) |
23 | human | 2263 | FGFR2 | fibroblast growth factor re... | NM_001320654.1 | 68.3% | 66.2% | (many diffs) |
24 | human | 2263 | FGFR2 | fibroblast growth factor re... | XM_024447892.1 | 52.4% | 52.4% | 0_1ins1173 |
25 | human | 2263 | FGFR2 | fibroblast growth factor re... | NR_073009.2 | 51.1% | (many diffs) | |
26 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507349.2 | 89.4% | 95.2% | (many diffs) |
27 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241673.2 | 89.4% | 95.2% | (many diffs) |
28 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_017321975.1 | 89.4% | 95.2% | (many diffs) |
29 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507350.3 | 89.2% | 95% | (many diffs) |
30 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | NM_010207.2 | 86.6% | 92.1% | (many diffs) |
31 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241674.2 | 86.4% | 91.9% | (many diffs) |
32 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507352.2 | 85.6% | 90.9% | (many diffs) |
33 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_017321976.1 | 85.4% | 90.7% | (many diffs) |
34 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_017321977.1 | 82.6% | 87.6% | (many diffs) |
35 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507354.3 | 79.6% | 85.3% | (many diffs) |
36 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241676.2 | 79.3% | 85.1% | (many diffs) |
37 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | NM_201601.2 | 77% | 82.8% | (many diffs) |
38 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241675.2 | 76.8% | 82.3% | (many diffs) |
39 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_011241677.2 | 76.7% | 82.6% | (many diffs) |
40 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XM_006507357.3 | 74.3% | 79.8% | (many diffs) |
41 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | NM_001347638.1 | 74% | 79.5% | (many diffs) |
42 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XR_001785477.1 | 33.7% | (many diffs) | |
43 | mouse | 14183 | Fgfr2 | fibroblast growth factor re... | XR_001785478.1 | 33.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2535
- ORF length:
- 2466
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtcagctgg ggtcgtttca tctgcctggt cgtggtcacc atggcaacct 121 tgtccctggc ccggccctcc ttcagtttag ttgaggatac cacattagag ccagaagagc 181 caccaaccaa ataccaaatc tctcaaccag aagtgtacgt ggctgcgcca ggggagtcgc 241 tagaggtgcg ctgcctgttg aaagatgccg ccgtgatcag ttggactaag gatggggtgc 301 acttggggcc caacaatagg acagtgctta ttggggagta cttgcagata aagggcgcca 361 cgcctagaga ctccggcctc tatgcttgta ctgccagtag gactgtagac agtgaaactt 421 ggtacttcat ggtgaatgtc acagatgcca tctcatccgg agatgatgag gatgacaccg 481 atggtgcgga agattttgtc agtgagaaca gtaacaacaa gagagcacca tactggacca 541 acacagaaaa gatggaaaag cggctccatg ctgtgcctgc ggccaacact gtcaagtttc 601 gctgcccagc cggggggaac ccaatgccaa ccatgcggtg gctgaaaaac gggaaggagt 661 ttaagcagga gcatcgcatt ggaggctaca aggtacgaaa ccagcactgg agcctcatta 721 tggaaagtgt ggtcccatct gacaagggaa attatacctg tgtagtggag aatgaatacg 781 ggtccatcaa tcacacgtac cacctggatg ttgtggagcg atcgcctcac cggcccatcc 841 tccaagccgg actgccggca aatgcctcca cagtggtcgg aggagacgta gagtttgtct 901 gcaaggttta cagtgatgcc cagccccaca tccagtggat caagcacgtg gaaaagaacg 961 gcagtaaata cgggcccgac gggctgccct acctcaaggt tctcaagcac tcggggataa 1021 atagttccaa tgcagaagtg ctggctctgt tcaatgtgac cgaggcggat gctggggaat 1081 atatatgtaa ggtctccaat tatatagggc aggccaacca gtctgcctgg ctcactgtcc 1141 tgccaaaaca gcaagcgcct ggaagagaaa aggagattac agcttcccca gactacctgg 1201 agatagccat ttactgcata ggggtcttct taatcgcctg tatggtggta acagtcatcc 1261 tgtgccgaat gaagaacacg accaagaagc cagacttcag cagccagccg gctgtgcaca 1321 agctgaccaa acgtatcccc ctgcggagac aggtaacagt ttcggctgag tccagctcct 1381 ccatgaactc caacaccccg ctggtgagga taacaacacg cctctcttca acggcagaca 1441 cccccatgct ggcaggggtc tccgagtatg aacttccaga ggacccaaaa tgggagtttc 1501 caagagataa gctgacactg ggcaagcccc tgggagaagg ttgctttggg caagtggtca 1561 tggcggaagc agtgggaatt gacaaagaca agcccaagga ggcggtcacc gtggccgtga 1621 agatgttgaa agatgatgcc acagagaaag acctttctga tctggtgtca gagatggaga 1681 tgatgaagat gattgggaaa cacaagaata tcataaatct tcttggagcc tgcacacagg 1741 atgggcctct ctatgtcata gttgagtatg cctctaaagg caacctccga gaatacctcc 1801 gagcccggag gccacccggg atggagtact cctatgacat taaccgtgtt cctgaggagc 1861 agatgacctt caaggacttg gtgtcatgca cctaccagct ggccagaggc atggagtact 1921 tggcttccca aaaatgtatt catcgagatt tagcagccag aaatgttttg gtaacagaaa 1981 acaatgtgat gaaaatagca gactttggac tcgccagaga tatcaacaat atagactatt 2041 acaaaaagac caccaatggg cggcttccag tcaagtggat ggctccagaa gccctgtttg 2101 atagagtata cactcatcag agtgatgtct ggtccttcgg ggtgttaatg tgggagatct 2161 tcactttagg gggctcgccc tacccaggga ttcccgtgga ggaacttttt aagctgctga 2221 aggaaggaca cagaatggat aagccagcca actgcaccaa cgaactgtac atgatgatga 2281 gggactgttg gcatgcagtg cccTCCCAGA GACCAACGTT CAAGCAGTTG GTAGAAGACT 2341 TGGATCGAAT TCTCACTCTC ACAACCAATG AGGAATACTT GGACCTCAGC CAACCTCTCG 2401 AACAGTATTC ACCTAGTTAC CCTGACACAA GAAGTTCTTG TTCTTCAGGA GATGATTCTG 2461 TTTTTTCTCC AGACCCCATG CCTTACGAAC CATGCCTTCC TCAGTATCCA CACATAAACG 2521 GCAGTGTTAA AACACCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2581 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2641 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTA GCGAGATACA TAGTTCGCTA 2701 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t