Construct: ORF TRCN0000465441
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014281.1_s317c1
- Derived from:
- ccsbBroadEn_03831
- DNA Barcode:
- CCTGACTAGTTGCCCATCCCATAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFT80 (57560)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465441
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57560 | IFT80 | intraflagellar transport 80 | NM_020800.3 | 100% | 100% | |
| 2 | human | 57560 | IFT80 | intraflagellar transport 80 | NM_001190241.2 | 82.3% | 82.3% | 0_1ins411 |
| 3 | human | 57560 | IFT80 | intraflagellar transport 80 | NM_001190242.1 | 82.3% | 82.3% | 0_1ins411 |
| 4 | human | 100174949 | TRIM59-IFT80 | TRIM59-IFT80 protein | NR_148401.1 | 63% | (many diffs) | |
| 5 | human | 100174949 | TRIM59-IFT80 | TRIM59-IFT80 protein | NR_148402.1 | 37.9% | (many diffs) | |
| 6 | human | 100174949 | TRIM59-IFT80 | TRIM59-IFT80 protein | NR_148403.1 | 36.3% | (many diffs) | |
| 7 | mouse | 68259 | Ift80 | intraflagellar transport 80 | NM_026641.2 | 88.2% | 94.5% | (many diffs) |
| 8 | mouse | 68259 | Ift80 | intraflagellar transport 80 | XM_006501966.3 | 88.2% | 94.5% | (many diffs) |
| 9 | mouse | 68259 | Ift80 | intraflagellar transport 80 | XM_017319702.1 | 85.1% | 91.5% | (many diffs) |
| 10 | mouse | 68259 | Ift80 | intraflagellar transport 80 | XM_006501967.3 | 81.3% | 87.1% | (many diffs) |
| 11 | mouse | 68259 | Ift80 | intraflagellar transport 80 | XR_001783737.1 | 48% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2400
- ORF length:
- 2331
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagactaaag atatctcttt taaaagaacc aaagcatcaa gaattagtaa 121 gctgtgtggg ctggactact gctgaagagc tgtattcatg tagtgatgat caccagatag 181 tgaagtggaa cttgttaacc agtgaaacaa ctcaaatagt aaagcttcct gatgatattt 241 accctattga ttttcactgg tttccaaaaa gtttgggtgt aaagaaacaa acccaggcag 301 aaagctttgt cctcacaagt tctgatggta aatttcatct gatttccaag ttaggaagag 361 tggaaaaaag tgtagaagct cactgtggag cagtacttgc aggaagatgg aattatgaag 421 gaacagcatt agttacagtt ggagaagatg gacaaataaa aatttggtca aagactggga 481 tgcttagatc aactttagct cagcaaggaa caccagtgta ttcagtagcg tggggccctg 541 attcagaaaa ggttctttat acagcaggca agcagctaat cattaaacct cttcaaccaa 601 atgctaaagt tttgcagtgg aaagctcatg atggcattat tttaaaagta gattggaact 661 cggtcaatga tcttatttta tctgctggtg aagactgtaa atataaggta tgggatagtt 721 acggccgccc actgtacaat tcacaacctc atgagcatcc cattacttca gttgcctggg 781 ctccagatgg agaattattt gctgttggat cgtttcatac tttacgcttg tgtgataaaa 841 ctgggtggtc atatgcatta gaaaaaccca acactggcag catatttaat attgcatggt 901 ctatcgatgg cactcagatt gctggagcct gtggaaatgg acatgtcgtt tttgcacatg 961 tggtggaaca acattgggag tggaaaaatt ttcaagtaac attaacgaaa agaagagcca 1021 tgcaggttcg taatgttctt aatgatgcag tggatttact ggaattccgt gatagagtca 1081 ttaaagcatc tttgaactat gcacacttag ttgtttcaac gtctcttcaa tgttacgtgt 1141 tctccacgaa gaactggaac acaccaatta tatttgatct caaagaagga actgttagtt 1201 tgattctgca ggcagaaaga cattttcttc ttgtagatgg tagtagtatc tatttatatt 1261 catatgaagg gcgctttatt tcatctccaa aatttcctgg aatgagaaca gatattctga 1321 atgcacagac tgtgtctttg agtaatgata ccatagcaat aagagacaaa gctgatgaaa 1381 aaataatctt cctctttgag gcatcaaccg gaaagccgtt aggtgatgga aagtttcttt 1441 ctcataagaa tgaaatcttg gaaattgctc tggatcaaaa aggacttacc aatgatagaa 1501 aaattgcttt cattgataaa aatagagatc tctgtatcac ttctgtgaaa cgatttggga 1561 aggaagaaca aattatcaag cttggaacaa tggtgcatac tttggcatgg aacgatacat 1621 gcaatatcct ttgtggactt caagatactc gatttatagt gtggtattac cccaatacag 1681 tttatgtgga cagagacatt ttgcctaaaa cattatatga aagggatgca agtgaattta 1741 gtaaaaatcc ccatattgtg agttttgttg gaaatcaagt aactattaga agagctgatg 1801 gctccctggt tcacatcagc ataacaccat atcctgctat tctccatgaa tatgtaagca 1861 gttcaaaatg ggaagatgct gtgagacttt gtcgctttgt taaggagcaa accatgtggg 1921 cttgtctagc tgctatggca gttgctaatc gagatatgac tactgcagaa atagcctatg 1981 cagcaattgg tgaaattgat aaggttcagt acatcaattc tataaaaaaT CTTCCATCTA 2041 AAGAATCAAA AATGGCCCAC ATACTACTGT TTAGTGGGAA CATACAGGAG GCTGAAATAG 2101 TACTTCTTCA GGCTGGCCTT GTTTATCAAG CAATCCAGAT CAATATTAAT CTCTACAACT 2161 GGGAAAGGGC ACTGGAATTG GCTGTAAAAT ACAAAACACA TGTTGATACA GTTCTTGCTT 2221 ACCGTCAAAA GTTTTTGGAG ACATTTGGTA AACAGGAAAC TAATAAACGA TACTTGCATT 2281 ATGCAGAAGG TCTCCAAATA GATTGGGAGA AAATCAAAGC CAAAATTGAG ATGGAAATTA 2341 CAAAAGAAAG AGAGCAATCA TCAAGCAGCC AATCCAGCAA GAGTATAGGT TTAAAGCCCT 2401 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2461 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2521 TTTATATATC TTGTGGAAAG GACGACCTGA CTAGTTGCCC ATCCCATAGA CGCGTTAAGT 2581 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt