Construct: ORF TRCN0000465498

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015459.1_s317c1
Derived from:
ccsbBroadEn_06606
DNA Barcode:
ACCCGCCCGAGAACTTTAAAAATA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MUSK (4593)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465498
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4593 MUSK muscle associated receptor ... NM_001166280.2 99.9% 100% 402G>A
2 human 4593 MUSK muscle associated receptor ... XM_017014734.1 98.9% 98.8% 402G>A;1127_1150del
3 human 4593 MUSK muscle associated receptor ... NM_001166281.2 98.6% 98.7% 402G>A;627_628ins30
4 human 4593 MUSK muscle associated receptor ... XM_005251995.3 89.8% 89.8% 402G>A;949_1212del
5 human 4593 MUSK muscle associated receptor ... XM_005251994.3 89% 88.9% 402G>A;949_1212del;1391_1414del
6 human 4593 MUSK muscle associated receptor ... XM_005251996.3 88.7% 88.7% 402G>A;627_628ins30;919_1182del
7 human 4593 MUSK muscle associated receptor ... NM_005592.4 87.9% 87.8% (many diffs)
8 human 4593 MUSK muscle associated receptor ... NM_001369398.1 57.3% 57.3% 0_1ins1002
9 human 4593 MUSK muscle associated receptor ... XM_011518708.2 56.7% 56.6% 0_1ins1002;125_148del
10 mouse 18198 Musk muscle, skeletal, receptor ... NM_001037129.1 79.1% 84.3% (many diffs)
11 mouse 18198 Musk muscle, skeletal, receptor ... NM_001037128.1 78.3% 83.5% (many diffs)
12 mouse 18198 Musk muscle, skeletal, receptor ... NM_001037130.1 78.2% 83.5% (many diffs)
13 mouse 18198 Musk muscle, skeletal, receptor ... XM_006537661.2 78.2% 83.4% (many diffs)
14 mouse 18198 Musk muscle, skeletal, receptor ... NM_010944.2 77.5% 82.7% (many diffs)
15 mouse 18198 Musk muscle, skeletal, receptor ... XM_006537659.2 77.5% 82.5% (many diffs)
16 mouse 18198 Musk muscle, skeletal, receptor ... NM_001037127.2 77% 82.1% (many diffs)
17 mouse 18198 Musk muscle, skeletal, receptor ... NM_001165996.1 38.3% 39.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2418
ORF length:
2349
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gagagagctc gtcaacattc cactggtaca tattcttact ctggttgcct
121tcagcggaac tgagaaactt ccaaaagctc ctgtcatcac cactcctctt gaaacagtgg
181atgccttagt tgaagaagtg gctactttca tgtgtgcagt ggaatcctac ccccagcctg
241agatttcctg gactagaaat aaaattctca ttaaactctt tgacacccgg tacagcatcc
301gggagaatgg gcagctcctc accatcctga gtgtggaaga cagtgatgat ggcatttact
361gctgcacggc caacaatggt gtgggaggag ctgtggagag ttgtggagcc ctgcaagtga
421agatgaaacc taaaataact cgtcctccca taaatgtgaa aataatagaa ggattaaaag
481cagtcctacc atgtactaca atgggtaatc ccaaaccatc agtgtcttgg ataaagggag
541acagccctct cagggaaaat tcccgaattg cagttcttga atctgggagc ttgaggattc
601ataacgtaca aaaggaagat gcaggacagt atcgatgtgt ggcaaaaaac agcctcggga
661cagcatattc caaagtggtg aagctggaag ttgaggaaga aagtgaaccc gaacaagata
721ctaaagtttt tgccaggatc ctgcgggctc ctgaatccca caatgtcacc tttggctcct
781ttgtgaccct gcactgtaca gcaacaggca ttcctgtccc caccatcacc tggattgaaa
841acggaaatgc tgtttcttct gggtccattc aagagagtgt gaaagaccga gtgattgact
901caagactgca gctgtttatc accaagccag gactctacac atgcatagct accaataagc
961atggggagaa gttcagtact gccaaggctg cagccaccat cagcatagca gaatggagag
1021agtactgctt ggcagtaaag gagctcttct gcgcaaaaga atggctggta atggaagaga
1081agacccacag aggactctac agatccgaga tgcatttgct gtccgtgcca gaatgcagca
1141agcttcccag catgcattgg gaccccacgg cctgtgccag actgccacat ctagcattcc
1201caccaatgac gtcctcaaag ccaagtgtgg acattccaaa tctgccttcc tcctcctctt
1261cttccttctc tgtctcacct acatactcca tgactgtaat aatctccatc atgtccagct
1321ttgcaatatt tgtgcttctt accataacta ctctctattg ctgccgaaga agaaaacaat
1381ggaaaaataa gaaaagagaa tcagcagcag taaccctcac cacactgcct tctgagctct
1441tactagatag acttcatccc aaccccatgt accagaggat gccgctcctt ctgaacccca
1501aattgctcag cctggagtat ccaaggaata acattgaata tgtgagagac atcggagagg
1561gagcgtttgg aagggtgttt caagcaaggg caccaggctt acttccctat gaacctttca
1621ctatggtggc agtaaagatg ctcaaagaag aagcctcggc agatatgcaa gcggactttc
1681agagggaggc agccctcatg gcagaatttg acaaccctaa cattgtgaag ctattaggag
1741tgtgtgctgt cgggaagcca atgtgcctgc tctttgaata catggcctat ggtgacctca
1801atgagttcct ccgcagcatg tcccctcaca ccgtgtgcag cctcagtcac agtgacttgt
1861ctatgagggc tcaggtctcc agccctgggc ccccacccct ctcctgtgct gagcagcttt
1921gcattgccag gcaggtggca gctggcatgg cttacctctc agaacgtaag tttgttcacc
1981gagatttagc caccaggaac tgcctggtgg gcgagaacat ggtggtgaaa attgccgact
2041ttggcctctc caggaacatc tacTCAGCAG ACTACTACAA AGCTAATGAA AACGACGCTA
2101TCCCTATCCG TTGGATGCCA CCAGAGTCCA TTTTTTATAA CCGCTACACT ACAGAGTCTG
2161ATGTGTGGGC CTATGGCGTG GTCCTCTGGG AGATCTTCTC CTATGGCCTG CAGCCCTACT
2221ATGGGATGGC CCATGAGGAG GTCATTTACT ACGTGCGAGA TGGCAACATC CTCTCCTGCC
2281CTGAGAACTG CCCCGTGGAG CTGTACAATC TCATGCGTCT ATGTTGGAGC AAGCTGCCTG
2341CAGACAGACC CAGTTTCACC AGTATTCACC GAATTCTGGA ACGCATGTGT GAGAGGGCAG
2401AGGGAACTGT GAGTGTCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
2461TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
2521GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACCCGCC CGAGAACTTT
2581AAAAATAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)